Fluorescence line-narrowing spectrometry: A versatile tool for the study of chemically initiated carcinogenesis

Jankowiak, Ryszard; Lu, Peiqi; Small, Gerald J.; Nishimoto, Marc; Varanasi, Usha; Kim, S.K.; Geacintov, Nicholas E.

doi:10.1016/0731-7085(90)80017-j

Cited by 18 publications

(13 citation statements)

References 32 publications

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“…An important point is that the multiplet origin structure is located in the region of the inhomogeneously broadened fluorescence origin produced under non-line-narrowing conditions (i.e., S2 «-S0 excitation). Most recently, this technique has been used for analysis of macromolecular DNA and globin damage from in vivo exposure to PAH (20,22). For example, it was shown that the major stable DNA adduct from the liver of fish exposed to high dosage levels of BP is derived from syn-BPDE (20), in agreement with previous work (23).…”

Section: Introductionsupporting

confidence: 77%

“…Most recently, this technique has been used for analysis of macromolecular DNA and globin damage from in vivo exposure to PAH (20,22). For example, it was shown that the major stable DNA adduct from the liver of fish exposed to high dosage levels of BP is derived from syn-BPDE (20), in agreement with previous work (23). At low dosage levels, the major adduct was found to originate from (+)-anti-BPDE (23,24).…”

Section: Introductionsupporting

confidence: 76%

“…At present, the highest resolution frequency domain technique available for the analysis of DNA-PAH adducts is laser-excited fluorescence line narrowing spectroscopy (FLNS) (13)(14)(15)(16)(17)(18)(19)(20)(21). FLNS is also applicable to nucleotide and nucleoside adducts (7,21).…”

Section: Introductionmentioning

confidence: 99%

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Comparative laser spectroscopic study of DNA and polynucleotide adducts from the (+)-anti-diol epoxide of benzo[a]pyrene

Lu¹,

Jeong²,

Jankowiak³

et al. 1991

Chem. Res. Toxicol.

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A recently developed methodology [Jankowiak, R., Lu, P., Small, G. J., and Geacintov, N. E. (1990) Chem. Res. Toxicol. 3, 39-46], which combines fluorescence line narrowing spectroscopy at 4.2 K with non-line-narrowed (S2----S0 laser excitation) fluorescence spectroscopy at 77 K and fluorescence quenching, is used to characterize adducts formed from (+)-anti-BPDE and the alternating copolymers poly(dG-dC).poly(dG-dC) and poly(dA-dT).poly(dA-dT), the nonalternating poly (dG).poly(dC), single-strand poly(dG), and the oligonucleotide d(ATATGTATA). Detailed comparisons of the fluorescence spectra and quenching (with acrylamide) of the properties of the adducts with those of (+)-anti-BPDE-DNA adducts are made. Fluorescence spectra of the trans and cis isomers of the adduct formed from guanosine monophosphate and the adducts of d(ATATGTATA) are used to assign the stereochemistry of the two major DNA adducts as trans-N2-dG moieties which occupy two different DNA sites. Evidence for the existence of minor cis-type guanine adducts is provided. Finally, a fourth type of DNA adduct (minor) is identified and assigned as trans-N6-dA.

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Section: Introductionsupporting

confidence: 77%

Section: Introductionsupporting

confidence: 76%

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Comparative laser spectroscopic study of DNA and polynucleotide adducts from the (+)-anti-diol epoxide of benzo[a]pyrene

Lu¹,

Jeong²,

Jankowiak³

et al. 1991

Chem. Res. Toxicol.

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“…Signi®cantly, the data in ®gure 33(c) do not have an indication of a rephasing feature, indicating that the coherences created after the ®rst and second Raman excitations do not have correlated dephasing dynamics [82]. There is, however, asymmetry between the t 23 and t 45 axes that suggests that the data are contaminate d with small contributions from parallel cascaded processes [82]. The amount of cascaded contamination in ®gure 33(c) can be found by ®rst modelling the frequency dependence expected for the cascaded processes using the known third-order response.…”

Section: Doubly Vibrationall Y Enhanced Six-wave Mixing (2d Raman)mentioning

confidence: 96%

“…The sequential cascade produces a signal that is symmetric in the t 23 and t 45 time delays since these delays control the intermediate coherences in the FWM processes. The parallel cascade process produces signals that are asymmetric in the time delays because the intermediate coherences depend on t 45 and t 23 ‡ t 45 respectively. (4) Finally, the phase of the output from the cascaded processes is shifted by p from the SWM output [125,157].…”

Section: Doubly Vibrationall Y Enhanced Six-wave Mixing (2d Raman)mentioning

confidence: 99%

Coherent multidimensional vibrational spectroscopy

Wright¹

2002

International Reviews in Physical Chemistry

109

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Coherent multidimensional vibrational spectroscopy (CMDVS) is an emerging ®eld that o ers new oportunities to probe speci®cally intramolecular and intermolecular interactions in complex samples with the improved resolution and selectivity expected of a multidimensional method. In particular, it provides structural and dynamical information about correlations between modes. It provides this information on the picosecond time scales characteristic of vibrational dephasing. CMDVS is based on the perturbations that are induced in a mode when other coupled modes are excited. It is the vibrational analogue to multidimensional nucler magnetic resonance (NMR). In this review, we present the background that led to the development of CMDVS, a brief summary of the di erent methods of non-linear laser spectroscopy and how they can achieve CMDVS, the relationships between CMDVS and multidimensional NMR, and the theory necessary to understand the experimental results. The experimental approaches reviewed fall into three main classes: non-degenerate four-wave mixing which is doubly vibrationally enhanced, degenerate four-wave mixing which is triply vibrationally enhanced and six-wave mixing which has three Raman transitions. CMDVS has been implemented in both the time domain and the frequency domain. Finally, the review describes possible extensions of CMDVS and speculates on its future.

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Identification of stereoisomeric benzo[a]pyrene tetrols by reversed-phase liquid chromatography coupled semi-ondashline to fluorescence line-narrowing spectroscopy

Kok¹,

Posthumus²,

Bakker³

et al. 1995

Analytica Chimica Acta

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Fluorescence line-narrowing spectrometry: A versatile tool for the study of chemically initiated carcinogenesis

Cited by 18 publications

References 32 publications

Comparative laser spectroscopic study of DNA and polynucleotide adducts from the (+)-anti-diol epoxide of benzo[a]pyrene

Comparative laser spectroscopic study of DNA and polynucleotide adducts from the (+)-anti-diol epoxide of benzo[a]pyrene

Coherent multidimensional vibrational spectroscopy

Identification of stereoisomeric benzo[a]pyrene tetrols by reversed-phase liquid chromatography coupled semi-ondashline to fluorescence line-narrowing spectroscopy

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