Fluorescence line narrowing spectroscopy in organic glasses containing PPB levels of polycyclic aromatic hydrocarbons

Fluorescence line narrowing-nonphotochemical hole burning spectrometry: femtomole detection and high selectivity for intact DNA-PAH adducts

“…Recent attempts at identifying DNA-PAH adducts have led to the development of several techniques, such as enzyme-linked immunosorbent assay (ELISA) and Randerath procedure (8)(9)(10). A fluorescence-based approach which affords high selectivity and one that has been applied to intact DNA adducts is fluorescence line narrowing (FLN) spectrometry (11)(12)(13)(14)(15)(16)(17)(18)(19)(20). In this methodology the 0893-228x/88/2701-0060$01.50/0 analytes are dissolved in a glass-forming solvent which is cooled to cryogenic temperatures (typically 4-20 K).…”

Laser-excited Shpol'skii spectroscopy for the selective excitation and determination of polynuclear aromatic hydrocarbons

“…In multiccmponent sanples, overlaps of absorption bands might necessitate use of another excitation wavelength for a particular conpound. Despite the numerous linear analytical curves that have been demonstrated, the potential analytical utility of the Shpol'skii effect for quantitation of trace levels of PAH connpounds have been questioned (71,74,96). These questioning assertions were based on classical Shpol'skii effect studies on the luminescence spectrum dependency on concentrations (49,57,60).…”

Laser excited Shpol'skii spectroscopy for the selective excitation and determination of polynuclear aromatic hydrocarbons

“…Selective excitation usually relies upon the separation of principal origins, that is, if the PAH origins are sufficiently separated, A ex can be set at a wavelength among them so that those PAHs with higher lying origins than Xex (in energy) cannot be electronically excited and will not fluoresce. Prom Figure 13, Despite the numerous linear analytical curves that have been demonstrated, the potential analytical utility of the Shpol'skii effect for quantitation of trace levels of PAH connpounds have been questioned (71,74,96). These questioning assertions were based on classical Shpol'skii effect studies on the luminescence spectrum dependency on concentrations (49,57,60).…”