Fluorescence molecular imaging systems for intraoperative image-guided surgery

Kim, Tae-Hoon; O’Brien, Connor; Choi, Hak; Jeong, Myung Yung

doi:10.1080/05704928.2017.1323311

Cited by 26 publications

(17 citation statements)

References 41 publications

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“…For the image sensor adopted in the prototype, the width (W) of the image area is 1.44 mm and the corresponding column number (N c ) is 1280. Substituting the above parameters into the derived Equations (6)- (8), and plotting the changes of distance from image plane to optical lens II (x 2 ), magnification of optical system (M), position of stripe in the captured image (c) with the changes of distance from specimen to optical lens I (x 1 ) in different d conditions, is shown in Figure 7.…”

Section: Simulationmentioning

confidence: 99%

“…It is widely recognized that intraoperative diagnosis technologies contribute to minimize surgical damage, preserve normal tissue and reduce the rate of recurrence for tumor resection surgery [1][2][3][4][5]. Optical imaging is characterized by low cost, nonionizing radiation and simple and high resolution [6][7][8], which is promising for design of an intraoperative diagnosis instrument. Despite advances in technology, the motion of living tissues or organs caused by breathing or heartbeat remains a major problem in intraoperative imaging by minimal invasive microscopic imaging devices (e.g., endomicroscopy) for pathologic diagnosis [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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A Compact High-Speed Image-Based Method for Measuring the Longitudinal Motion of Living Tissues

Yang

Liao

et al. 2020

Sensors

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Intraoperative imaging of living tissue at the cell level by endomicroscopy might help surgeons optimize surgical procedures and provide individualized treatments. However, the resolution of the microscopic image is limited by the motion of living tissue caused by heartbeat and respiration. An active motion compensation (AMC) strategy has been recognized as an effective way to reduce, or even eliminate, the influence of tissue movement for intravital fluorescence microscopy (IVM). To realize the AMC system, a high-speed sensor for measuring the motion of tissues is needed. At present, state-of-the-art commercialized displacement sensors are not suitable to apply in minimally invasive imaging instruments to measure the motion of living tissues because of the size problem, range of measurement or the update rate. In this study, a compact high-speed image-based method for measuring the longitudinal motion of living tissues is proposed. The complexity of the proposed method is the same as that of the traditional wide-field fluorescent microscopy (WFFM) system, which makes it easy to be miniaturized and integrated into a minimally invasive imaging instrument. Experimental results reveal that the maximum indication error, range of measurement and the sensitivity of the laboratory-built experimental prototype is 150 μm, 6 mm and −211.46 mm−1 respectively. Experimental results indicate that the proposed optical method is expected to be used in minimally invasive imaging instruments to build an AMC system.

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Section: Simulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Compact High-Speed Image-Based Method for Measuring the Longitudinal Motion of Living Tissues

Yang

Liao

et al. 2020

Sensors

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“…[40] However, some fundamental challenges faced by FLI are limited resolution, limited quantification, limited depth of penetration, and limited availability of targeted contrast agents. [41,42] …”

Section: Intraoperative Imaging Systemsmentioning

confidence: 99%

“…[44] These FLARE imaging systems are relatively cheap and can be miniaturized to the desktop by integrating camera and optical filter sets. [41] …”

Section: Intraoperative Imaging Systemsmentioning

confidence: 99%

Real‐Time Imaging of Brain Tumor for Image‐Guided Surgery

Kang

Baek

et al. 2018

Adv Healthcare Materials

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The completion of surgical resection is a key prognostic factor in brain tumor treatment. This requires surgeons to identify residual tumors in theater as well as to margin the proximity of the tumor to adjacent normal tissue. Subjective assessments, such as texture palpation or visual tissue differences, are commonly used by oncology surgeons during resection to differentiate cancer lesions from normal tissue, which can potentially result in either an incomplete tumor resection, or accidental removal of normal tissue. Moreover, malignant brain tumors are even more difficult to distinguish from normal brain tissue, and resecting noncancerous tissue may create neurological defects after surgery. To optimize the resection margin in brain tumors, a variety of intraoperative guidance techniques are developed, such as neuronavigation, magnetic resonance imaging, ultrasound, Raman spectroscopy, and optical fluorescence imaging. When combined with appropriate contrast agents, optical fluorescence imaging can provide the neurosurgeon real-time image guidance to improve resection completeness and to decrease surgical complications.

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“…In recent years, a number of fluorescence imaging systems have been developed for use with ICG. Several of these systems have reached preclinical or clinical stages, including the Curadel FLARE, Novadaq Spy Elite, Hamamatsu PDE-NEO II, Fluoptics Fluobeam, Intuitive Surgical da Vinci Firefly, and Nawoo Vision FIAT-L imaging systems [36,37], further emphasizing the growing interest in NIR fluorescence imaging, particularly based on ICG.…”

mentioning

confidence: 99%

Near-Infrared-Fluorescent Erythrocyte-Mimicking Particles: Physical and Optical Characteristics

Tang

Partono

Anvari

2019

IEEE Trans. Biomed. Eng.

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Exogenous fluorescent materials activated by near-infrared (NIR) light can offer deep optical imaging with sub-cellular resolution, and enhanced image contrast. We have engineered NIR particles by doping hemoglobin-depleted erythrocyte ghosts (EGs) with indocyanine green (ICG). We refer to these optical particles as NIR erythrocyte-mimicking transducers (NETs). A particular feature of NETs is that their diameters can be tuned from micrometer to nanometer scale, thereby, providing a capability for broad NIR biomedical imaging applications. Herein, we investigate the effects of ICG concentration on key material properties of micrometer-sized NETs, and nanometer-sized NETs fabricated by either sonication or mechanical extrusion of EGs. The zeta potentials of NETs do not vary significantly with ICG concentration, suggesting that ICG is completely encapsulated within NETs regardless of particle size or ICG concentration. Loading efficiency of ICG into the NETs monotonically decreases with increasing values of ICG concentration. Based on quantitative analyses of the fluorescence emission spectra of the NETs, we determine that 20 μM ICG utilized during fabrication of NETs presents an optimal concentration that maximizes the integrated fluorescence emission for micrometer- and nanometer-sized NETs. Encapsulation of ICG in these constructs also enhanced the fluorescence stability and quantum yield of ICG. These results guide the engineering of NETs with maximal NIR emission for imaging applications such as fluorescence-guided tumor resection, and real-time angiography.

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Fluorescence molecular imaging systems for intraoperative image-guided surgery

Cited by 26 publications

References 41 publications

A Compact High-Speed Image-Based Method for Measuring the Longitudinal Motion of Living Tissues

A Compact High-Speed Image-Based Method for Measuring the Longitudinal Motion of Living Tissues

Real‐Time Imaging of Brain Tumor for Image‐Guided Surgery

Near-Infrared-Fluorescent Erythrocyte-Mimicking Particles: Physical and Optical Characteristics

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