Fluorescence of hematoxylin and eosin-stained histological sections of the human spleen

Jakubovský, J; Guller, Ladislav; Černá, Magdaléna; Balážová, Katarína; Polák, Štefan; Jakubovská, Vlasta; Babál, Pavel

doi:10.1078/0065-1281-00684

Cited by 27 publications

(18 citation statements)

References 3 publications

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“…Classically, contrast enhancement was performed by using black and white photographs with a green filter. For selective visualization of eosinophilic structures, fluorescence has been employed for the visualization of a wide variety of structures (Carle 1981;Espada et al 1993;de Carvalho and Taboga 1996;Jakubovsky et al 2002;Wu et al 2002;De Rossi et al 2007). These fluorescence techniques produce clear images only when confocal microscopy is used (de Carvalho and Taboga 1996;Wu et al 2002).…”

Section: Discussionmentioning

confidence: 99%

The eosin-shadow method: a selective enhancement of light-microscopic visualization of pancreatic zymogen granules on hematoxylin–eosin sections

et al. 2009

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We developed a novel method for enhancing light-microscopic visualization of pancreatic zymogen granules in a selective manner on hematoxylin and eosin-stained sections. By using an absorption filter that transmits light with wavelength from 510 to 550 nm, corresponding to the narrow absorption spectrum of eosin, only eosinophilic tissue and cellular components were remarkably highlighted as distinct shadows against lighter background consisting of basophilic components. Using a pair of mirror sections of the pancreas, immunocytochemistry with anti-amylase antibody confirmed that the shadows observed through the filter represented zymogen granules. Immersion in formalin for 36 h at room temperature was the optimal fixation condition. Here we designate the procedure as the "eosin-shadow method" and propose that this technique is convenient and useful to help investigators identify zymogen granules more easily in routine pathological examination and histological studies.

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Section: Discussionmentioning

confidence: 99%

The eosin-shadow method: a selective enhancement of light-microscopic visualization of pancreatic zymogen granules on hematoxylin–eosin sections

et al. 2009

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“…Examination of the extracellular reticulum can help, since this net from reticular fibres is typical for periarterial lymphatic sheaths (region with T-lymphocytes) and is absent in lymphatic follicles (typical region with B-lymphocytes). The fluorescence method for haematoxylin-eosin stained slides can be utilized in diagnostics (Jakubovský et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of human spleen in transmission and scanning electron microscope

2009

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Despite new information concerning functional morphology of spleen, there are still some inaccuracies mostly regarding the spleen blood circulation. Billroth's (splenic) cords are formed from three-dimensional network of fibroblastic reticular cells located among branched sinuses. Results from our study using scanning electron microscopy confirm an intimate contact between adjacent reticular cells and erythrocytes. Arterial terminals can be observed in the Billroth's cords. The wall of sinuses reminds a sieve and it is lined with a special type of endothelium. In electron microscope, endothelial cells look like rods oriented parallel to the longitudinal axis of sinuses. Based on our observations fibroblastic reticular cells change to fixed phagocytes under no circumstances, hence they do not participate in phagocytosis. They may have a recognition function for cells circulating around them. According to our opinion, the open and the closed blood circulation are present in the human spleen simultaneously. Blood flowing in the closed circulation can help "absorption" of extra-vascular liquid and the blood elements into the vascular lumen. Due to sporadic occurrence of smooth muscle cells in the capsule and trabeculae, we assume that human spleen is not a blood reservoir, unlike the spleen in some other animals.

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“…The said characteristics enable a closer characterization of the circulation components of the spleen. Jakubovský et al (2002) showed a useful utilization of eosin -stained slides for the demonstration of splenic circulation. Central arteries, arterial endings or venous sinuses can be all observed by fluorescent microscopy.…”

Section: Discussionmentioning

confidence: 99%

Some possibilities of representing microcirculation in human spleen

et al. 2009

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The representation of microcirculation can be approached in several ways. One of the possibilities is to represent the endothelium (endothelial or sinus lining cells) and their basement membrane on the basis of detecting the known components and the expression of the surface antigenes by the methods of immuno-, enzyme-or lectino-histochemical analysis, or by staining or impregnation histological methods. The other possibility is the examination of samples by transmission and scanning electron microscopy. For three-dimensional demonstration corrosion casts techniques or laser scanning confocal microscopy can be used. In this paper we describe the survey of immuno-, enzyme-and lectino-histochemical characteristics of selected components of microcirculation and our own results of its demonstration in human spleen.

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Fluorescence of hematoxylin and eosin-stained histological sections of the human spleen

Cited by 27 publications

References 3 publications

The eosin-shadow method: a selective enhancement of light-microscopic visualization of pancreatic zymogen granules on hematoxylin–eosin sections

The eosin-shadow method: a selective enhancement of light-microscopic visualization of pancreatic zymogen granules on hematoxylin–eosin sections

Ultrastructure of human spleen in transmission and scanning electron microscope

Some possibilities of representing microcirculation in human spleen

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