The eosin-shadow method: a selective enhancement of light-microscopic visualization of pancreatic zymogen granules on hematoxylin–eosin sections

Takei, Shiro; Tokuhira, Yu; Shimada, Atsuyoshi; Hosokawa, Masanori; Fukuoka, Shin-Ichi

doi:10.1007/s12565-009-0067-5

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…It was previously shown that autofluorescence of eosin Y in H & E-stained pancreatic sections provides an excellent method for selectively identifying acinar cell zymogen granules (37,39). Fluorescence analysis of pancreatic sections confirmed our previous report (1) that TPN induces a striking loss of granular staining in the apical cytoplasm of acini indicating a loss of zymogen granules (Fig.…”

Section: Resultssupporting

confidence: 87%

Intravenous or luminal amino acids are insufficient to maintain pancreatic growth and digestive enzyme expression in the absence of intact dietary protein

Baumler¹,

Koopmann

Thomas³

et al. 2010

American Journal of Physiology-Gastrointestinal and Liver Physi

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We previously reported that rats receiving total parenteral nutrition (TPN) undergo significant pancreatic atrophy characterized by reduced total protein and digestive enzyme expression due to a lack of intestinal stimulation by nutrients (Baumler MD, Nelson DW, Ney DM, Groblewski GE. Am J Physiol Gastrointest Liver Physiol 292: G857-G866, 2007). Essentially identical results were recently reported in mice fed protein-free diets (Crozier SJ, D'Alecy LG, Ernst SA, Ginsburg LE, Williams JA. Gastroenterology 137: 1093-1101, 2009), provoking the question of whether reductions in pancreatic protein and digestive enzyme expression could be prevented by providing amino acids orally or by intravenous (IV) infusion while maintaining intestinal stimulation with fat and carbohydrate. Controlled studies were conducted in rats with IV catheters including orally fed/saline infusion or TPN-fed control rats compared with rats fed a protein-free diet, oral amino acid, or IV amino acid feeding, all with oral carbohydrate and fat. Interestingly, neither oral nor IV amino acids were sufficient to prevent the pancreatic atrophy seen for TPN controls or protein-free diets. Oral and IV amino acids partially attenuated the 75-90% reductions in pancreatic amylase and trypsinogen expression; however, values remained 50% lower than orally fed control rats. Lipase expression was more modestly reduced by a lack of dietary protein but did respond to IV amino acids. In comparison, chymotrypsinogen expression was induced nearly twofold in TPN animals but was not altered in other experimental groups compared with oral control animals. In contrast to pancreas, protein-free diets had no detectable effects on jejunal mucosal villus height, total mass, protein, DNA, or sucrase activity. These data underscore that, in the rat, intact dietary protein is essential in maintaining pancreatic growth and digestive enzyme adaptation but has surprisingly little effect on small intestinal mucosa.

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Section: Resultssupporting

confidence: 87%

Intravenous or luminal amino acids are insufficient to maintain pancreatic growth and digestive enzyme expression in the absence of intact dietary protein

Baumler¹,

Koopmann

Thomas³

et al. 2010

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Moreover, no zymogen granules are detected using fluorescent eosin staining (Fig. 2C), which would indicate the presence of pepsinogens 31 . However, gene expression analyses revealed that the genes encoding the catalytic α-subunit ( atp4a) and the non-catalytic β-subunit ( atp4b) of H + /K + ATPase are expressed in the epithelium of the prospective stomach from st.27 onwards (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…canicula and detect the presence of acid mucins (blue) and neutral-acidic mucins (magenta). Eosin fluorescence allowed visualization of eosinophilic zymogen granules typically found in the acinar cells of gastric glands 31 . Immunohistochemistry (IHC), with rabbit polyclonal antibody (C2) against the H + /K + ATPase α - or catalytic subunit (HKα1) 53 , was used to identify the onset of HKα1 production during S .…”

Section: Methodsmentioning

confidence: 99%

Molecular ontogeny of the stomach in the catshark Scyliorhinus canicula

Gonçalves

Freitas

Ferreira

et al. 2019

Sci Rep

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The origin of extracellular digestion in metazoans was accompanied by structural and physiological alterations of the gut. These adaptations culminated in the differentiation of a novel digestive structure in jawed vertebrates, the stomach. Specific endoderm/mesenchyme signalling is required for stomach differentiation, involving the growth and transcription factors: 1) Shh and Bmp4, required for stomach outgrowth; 2) Barx1, Sfrps and Sox2, required for gastric epithelium development and 3) Cdx1 and Cdx2, involved in intestinal versus gastric identity. Thus, modulation of endoderm/mesenchyme signalling emerges as a plausible mechanism linked to the origin of the stomach. In order to gain insight into the ancient mechanisms capable of generating this structure in jawed vertebrates, we characterised the development of the gut in the catshark Scyliorhinus canicula. As chondrichthyans, these animals retained plesiomorphic features of jawed vertebrates, including a well-differentiated stomach. We identified a clear molecular regionalization of their embryonic gut, characterised by the expression of barx1 and sox2 in the prospective stomach region and expression of cdx1 and cdx2 in the prospective intestine. Furthermore, we show that gastric gland development occurs close to hatching, accompanied by the onset of gastric proton pump activity. Our findings favour a scenario in which the developmental mechanisms involved in the origin of the stomach were present in the common ancestor of chondrichthyans and osteichthyans.

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“…Histological sections from the tail of the pancreas from each experimental group were stained with hematoxylin and eosin (Sigma Aldrich) and periodic acid–Schiff (PAS) (Sigma Aldrich) staining without diastase digestion (Fig. 2) using standard protocols and then evaluated . Sections were examined under a digital inverted light microscope (EVOS TM xl core; Fisher Scientific, Waltham, MA, USA) to compare acinar size, structure, and morphology (Fig.…”

Section: Methodsmentioning

confidence: 99%

Impact of donor age and weaning status on pancreatic exocrine and endocrine tissue maturation in pigs

Krishnan

Truong

Gerges

et al. 2015

Xenotransplantation

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The eosin-shadow method: a selective enhancement of light-microscopic visualization of pancreatic zymogen granules on hematoxylin–eosin sections

Cited by 3 publications

References 9 publications

Intravenous or luminal amino acids are insufficient to maintain pancreatic growth and digestive enzyme expression in the absence of intact dietary protein

Intravenous or luminal amino acids are insufficient to maintain pancreatic growth and digestive enzyme expression in the absence of intact dietary protein

Molecular ontogeny of the stomach in the catshark Scyliorhinus canicula

Impact of donor age and weaning status on pancreatic exocrine and endocrine tissue maturation in pigs

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