Fluorescence of phosphotyrosine — Terbium(III) complexes

Selective Detection of Phosphotyrosine in the Presence of Various Phosphate‐Containing Biomolecules with the Aid of a Terbium(III) Complex

“…It is also noteworthy that GMP hardly increased the luminescence intensity from the Tb III ⋅ DOTAM (the green line in Figure 1 A). When Tb III ion itself was used, pTyr enhanced the luminescence from the ion, as previously reported by Niedbalski et al In that case, however, GMP enormously enhanced the luminescence (see the green line in Figure 1 B), as also described in the paper 10. In the absence of DOTAM, GMP strongly binds to the Tb III ion through multiple coordination both of the phosphate residue and of the guanine ring (N7 and O6; Scheme ) 7a.…”

“…Because of this characteristic sensitizing process, both lanthanide ions and their complexes have been used as sensing probes 6–12. In the case of pTyr detection, Niedbalski et al reported that luminescence from the Tb III ion is promoted by interaction with pTyr but little affected by other amino acids, including pSer/pThr 10. However, the Tb III ion also bound other molecules—guanosine‐5′‐monophosphate (GMP), for example—and emitted notable luminescence (see below).…”

Selective detection of tyrosine-containing proximally phosphorylated motifs using an antenna-free Tb3+ luminescent sensor

“…However, as the pTAY peptide did not induce significant signal enhancement, it suggested that direct phosphorylation on the Y antenna may be required. 6 Consistent with previous studies 18,19,21 we observed no Tb 3+ luminescence enhancement in response to some biologically relevant phospho-anions including ATP, AMP, and PPi. Although the signal was not observed, Tb 3+ was expected to bind the pSpS phosphorylated site.…”

“…Hamachi et al and Molecular Probes Inc.) have achieved significant progress in the detection of mono-phosphorylated peptide and protein sequences. [18][19][20][21][22][23] The signal selectivity of Tb 3+ for pY arises from the luminescence sensitization of the Tb 3+ ion (Scheme 1). [14][15][16][17] Although transition metal-based luminescent phospho-protein sensors were demonstrated to be effective and versatile in their applications, they cannot be readily optimized to recognize among the residues that are phosphorylated, i.e.…”

Binuclear Terbium(III) Complex as a Probe for Tyrosine Phosphorylation

“…These factors are more critical if a Tb III ion (without any ligand) is used; nucleotides and nucleic acids show enormous antenna effects. [14,15] To obtain the signal from pTyr precisely, these background signals must be reduced.…”