Fluorescence of Tetracycline Antibiotics in Bone

Milch, Robert Austin; Rall, David P.; Tobie, John E.

doi:10.2106/00004623-195840040-00013

Cited by 306 publications

(73 citation statements)

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“…This has been performed by using tritiated thymidine (Tonna & Cronkite 1963). However, since Milch et al (1958) demonstrated the binding of tetracycline to regions of new bone formation this method as well as the various combinations of different fluorochrome dyes has gained increasing popularity in the study of osteogenic processes.…”

Section: Discussionmentioning

confidence: 99%

The Effect of the Thickness of the Cortical Bone on Bone Formation by Osteoperiosteal Grafts:A Comparative Study Employing Routine Histological Stains and Triple Fluorochrome Labelling

Poussa

Rubak

Ritsilä

1980

Acta Orthopaedica Scandinavica

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Section: Discussionmentioning

confidence: 99%

The Effect of the Thickness of the Cortical Bone on Bone Formation by Osteoperiosteal Grafts:A Comparative Study Employing Routine Histological Stains and Triple Fluorochrome Labelling

Poussa

Rubak

Ritsilä

1980

Acta Orthopaedica Scandinavica

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“…OTC is known to localize and persist specifically in calcified structures [38,39]. Fluorescence was excited by epi-illumination using an excitation filter (35-1 8 nm), dichroic mirror (450 nm) and barrier filter (495 nm) and recorded digitally by a color digital camera (Axiocam, Zeiss.…”

Section: Bone Formation During Bone Defect Healingmentioning

confidence: 99%

Femur window—a new approach to microcirculation of living bone in situ

Hansen-Algenstaedt

Schaefer

Wolfram

et al. 2005

Journal Orthopaedic Research

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Background: The processes of osteogenesis, bone remodelling, fracture repair and metastasis to bone are determined by complex sequential interactions involving cellular and microcirculatory parameters. Consequently studies targeting the analysis of microcirculatory parameters on such processes should mostly respect these complex conditions. However these conditions could not yet be achieved in vitro and therefore techniques that allow a long-term observation of functional and structural parameters of microcirculation in bone in vivo at a high spatial resolution are needed to monitor dynamic events, such as fracture healing, bone remodelling and tumor metastasis.Methods: We developed a bone chamber implant (femur window) for long-term intravital microscopy of pre-existing bone and its microcirculation at an orthotopic site in mice preserving the mechanical properties of bone. After bone chamber implantation vascular density, vessel diameter, vessel perfusion, vascular permeability and leukocyte-endothelial interactions (LEIS) in femoral bone tissue of c57-black mice ( n = 11) were measured quantitatively over 12 days using intravital fluorescence microscopy. Furthermore a model for bone defect healing and bone metastasis in the femur window was tested.Results: Microvascular permeability and LEIS showed initially high values after chamber implantation followed by a significant decrease to a steady state at day 6 and 12, whereas structural parameters remained unaltered. Bone defect healing and tumor growth was observed over 12 and 90 days respectively. Conclusion:The new femur window design allows a long-term analysis of structural and functional properties of bone and its microcirculation quantitatively at a high spatial resolution. Altered functional parameters of microcirculation after surgical procedures and their time dependent return to a steady state underline the necessity of long-term observations to achieve unaltered microcirculatory parameters. Dissection of the complex interactions between bone and microcirculation enables us to evaluate physiological and pathological processes of bone and may give new insights especially in dynamic events e.g. fracture healing, bone remodeling and tumor metastasis.

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“…When bone growth occurs following treatment with an adequate dose of OTC, OTC is incorporated into mineralizing bone (Milch 1958), making it possible to determine the frequency of LAG deposition by comparing the number of LAGs exterior to the OTC mark with the number of years the sea turtle was at large. In captive experiments with farm-reared green sea turtles, Frazier (1985) found that doses ranging from 5 to 28 mg kg -1 resulted in fluorescent lines in the peripheral bone tissue, with higher doses resulting in greater fluorescence.…”

Section: Introductionmentioning

confidence: 99%

Validation of annual skeletal marks in green sea turtles Chelonia mydas using tetracycline labeling

Snover¹,

Hohn²,

Goshe³

et al. 2011

Aquat. Biol.

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The technique of skeletochronology has been widely applied to age and growth studies of most species of sea turtles, although direct validation of annual growth marks has only been demonstrated for loggerheads Caretta caretta and Kemp's ridleys Lepidochelys kempii. In this study we present validation of annual growth mark deposition in green sea turtles Chelonia mydas. Fourteen green sea turtles, previously captured in nearshore waters in the main Hawaiian Islands and injected with oxytetracycline (OTC), were recovered stranded, and the humeri were prepared for skeletochronological analysis. Each sea turtle had received a single dose of 25 mg kg -1 OTC, with half injected into each pectoral muscle. Seven of these sea turtles either were not at large long enough or did not grow enough for either the OTC mark to be discernable in bone cross-sections or for a growth mark to have been deposited after the OTC mark. Based on the hypothesis of one mark per year, the expected number of growth marks was visible exterior to the OTC mark in the humeri of the 7 remaining sea turtles, providing strong validation that growth marks are annual. These results support the use of skeletochronology for age and growth rate estimates in green sea turtles and the need for validation to ensure accurate interpretation of growth marks.

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Fluorescence of Tetracycline Antibiotics in Bone

Cited by 306 publications

References 0 publications

The Effect of the Thickness of the Cortical Bone on Bone Formation by Osteoperiosteal Grafts:A Comparative Study Employing Routine Histological Stains and Triple Fluorochrome Labelling

The Effect of the Thickness of the Cortical Bone on Bone Formation by Osteoperiosteal Grafts:A Comparative Study Employing Routine Histological Stains and Triple Fluorochrome Labelling

Femur window—a new approach to microcirculation of living bone in situ

Validation of annual skeletal marks in green sea turtles Chelonia mydas using tetracycline labeling

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