Fluorescence optical imaging in anticancer drug delivery

Etrych, Tomáš; Lucas, Henrike; Janoušková, Olga; Chytil, Petr; Mueller, Thomas; Mäder, Karsten

doi:10.1016/j.jconrel.2016.02.022

Cited by 118 publications

(67 citation statements)

References 111 publications

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“…This design permitted the evaluation of the system at the cell, tissue, and whole body levels and provided detailed knowledge of enzyme‐responsive drug release. We anticipate that the noninvasive techniques of conjugate evaluation will be used for the characterization of additional drug delivery systems and ultimately contribute to the design of effective polymer therapeutics.…”

Section: Discussionmentioning

confidence: 99%

FRET Imaging of Enzyme‐Responsive HPMA Copolymer Conjugate

Zhang

Yang

Radford

et al. 2016

Macromolecular Bioscience

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Fluorescence resonance energy transfer (FRET) is applied to investigate the enzyme-responsive payload release from a macromolecular therapeutic. The donor Cy5 is attached to the N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer backbone and the acceptor Cy7 is bound to the termini of enzyme-sensitive peptide side chains. Upon exposure to an enzyme, the bond between the peptide and Cy7 is cleaved, thereby leading to the loss of FRET signal. This enzyme response is visualized at the cell, tissue and whole-body levels. The in vitro results demonstrate that high expression of cathepsin B in tumor cells induces effective release of the drug model from conjugates resulting in a high concentration of payload inside tumor cells. The in vivo and ex vivo images show that the conjugate releases drug model faster in the ovarian tumor than in the normal tissues. The information will enhance the understanding of enzyme-responsive polymer carriers and help to shape their design.

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Section: Discussionmentioning

confidence: 99%

FRET Imaging of Enzyme‐Responsive HPMA Copolymer Conjugate

Zhang

Yang

Radford

et al. 2016

Macromolecular Bioscience

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“…For example, MRI, PET, CT, and other imaging systems for cancer diagnosis are ineffective to detect small metastatic tumors because contrast agents used for these diagnostic techniques fail to diffuse into such tumors and stay within them for a sufficient time (59). Fluorescent dyes are also widely used to visualize cancer cells and identify small tumors during intraoperative imaging, but common issues include poor diagnostic properties such as low signal-to-noise ratios and short half-lives in vivo (60). In order to overcome these limitations, nanoparticles entrapping small molecule imaging dyes have been recently developed.…”

Section: Discussionmentioning

confidence: 99%

Development of Halofluorochromic Polymer Nanoassemblies for the Potential Detection of Liver Metastatic Colorectal Cancer Tumors Using Experimental and Computational Approaches

et al. 2017

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Purpose To develop polymer nanoassemblies (PNAs) modified with halofluorochromic dyes to allow for the detection of liver metastatic colorectal cancer (CRC) to improve therapeutic outcomes. Methods We combine experimental and computational approaches to evaluate macroscopic and microscopic PNA distributions in patient-derived xenograft primary and orthotropic liver metastatic CRC tumors. Halofluorochromic and non-halofluorochromic PNAs (hfPNAs and n-hfPNAs) were prepared from poly(ethylene glycol), fluorescent dyes (Nile blue, Alexa546, and IR820), and hydrophobic groups (palmitate), all of which were covalently tethered to a cationic polymer scaffold [poly(ethylene imine) or poly(lysine)] forming particles with an average diameter < 30 nm. Results Dye-conjugated PNAs showed no aggregation under opsonizing conditions for 24 h and displayed low tissue diffusion and cellular uptake. Both hfPNAs and n-hfPNAs accumulated in primary and liver metastatic CRC tumors within 12 h post intravenous injection. In comparison to n-hfPNAs, hfPNAs fluoresced strongly only in the acidic tumor microenvironment (pH<7.0) and distinguished small metastatic CRC tumors from healthy liver stroma. Computational simulations revealed that PNAs would steadily accumulate mainly in acidic (hypoxic) interstitium of metastatic tumors, independently of the vascularization degree of the tissue surrounding the lesions. Conclusion The combined experimental and computational data confirms that hfPNAs detecting acidic tumor tissue can be used to identify small liver metastatic CRC tumors with improved accuracy.

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“…[3][4][5] It not only ensures chemical and biological stabilities, but also maintains the controllable release. [6,7] Furthermore, making the drug concentration controllably www.advancedsciencenews.com www.mcp-journal.de Reagent Factory (Guangzhou, China). All these reagents were used without further purification.…”

Section: Introductionmentioning

confidence: 99%

Direct Metal‐Free Preparation of Functionalizable Polylactic Acid‐Ethisterone Conjugates in a One‐Pot Approach

Luo

Cao

et al. 2019

Macro Chemistry & Physics

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A series of polylactic acid (PLA) conjugates (PLA‐ES) are synthesized via direct melt polycondensation (DMP) of lactic acid (LA) with ethisterone (ES) in the presence of 0.3 wt% toluenesulfonic acid at 160 °C for 6 h. The drug content of PLA conjugates can be simply adjustable. Further functionalization via Click chemistry is also achieved to extend the biomedicine application of biopolymer based drug delivery.

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Fluorescence optical imaging in anticancer drug delivery

Cited by 118 publications

References 111 publications

FRET Imaging of Enzyme‐Responsive HPMA Copolymer Conjugate

FRET Imaging of Enzyme‐Responsive HPMA Copolymer Conjugate

Development of Halofluorochromic Polymer Nanoassemblies for the Potential Detection of Liver Metastatic Colorectal Cancer Tumors Using Experimental and Computational Approaches

Direct Metal‐Free Preparation of Functionalizable Polylactic Acid‐Ethisterone Conjugates in a One‐Pot Approach

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