Fluorescence quenching in micellar solutions and its application to the determination of aggregation numbers

Infelta, Pierre P.

doi:10.1016/0009-2614(79)85092-7

Cited by 252 publications

(169 citation statements)

References 3 publications

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“…In most instances the fluorescence decay curves in the presence of quencher were found to have a long time slope which was identical to that in the absence of quencher, within the experimental error. This behavior indicated that the probe and quencher distributions were frozen on the fluorescence time scale (32)(33)(34)(35). In this case the usual four-parameter decay equation was fitted to the decay curves.…”

Section: Methodsmentioning

confidence: 99%

Mixed Micellization of Dimeric (Gemini) Surfactants and Conventional Surfactants

Alargova

Kochijashky

Sierra

et al. 2001

Journal of Colloid and Interface Science

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Section: Methodsmentioning

confidence: 99%

Mixed Micellization of Dimeric (Gemini) Surfactants and Conventional Surfactants

Alargova

Kochijashky

Sierra

et al. 2001

Journal of Colloid and Interface Science

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“…To measure the most important physico-chemical parameters of detergents or mixtures of detergents, the CMC and the aggregation number, respectively, we developed a new assay that is based on a combination and improvement of existing protocols [8]- [10]. Due to the information gained with this assay, it is now possible to adjust buffer conditions of the purification and/or subsequent activity assay, in a controlled and almost highthroughput manner.…”

Section: Resultsmentioning

confidence: 99%

“…To calculate aggregation numbers of detergents, pyrene quenching has been used extensively in the past [8]- [10]. Hoechst33342 absorption spectra and pyrene fluorescence emission spectra overlap.…”

Section: Resultsmentioning

confidence: 99%

“…Here, we present a simple "tool-box" combining different, based on already published procedures [1] [8]- [10] to determine the CMC values as well as and most importantly the aggregation numbers of detergents or detergent mixtures. As a representative detergent, we choose DDM as this is the most widely used detergent in membrane protein purification, analysis and crystallization [11].…”

Section: Introductionmentioning

confidence: 99%

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Analyzing the Physico-Chemical Parameters of Detergents and Detergent Mixtures

Tschapek¹,

Smits²,

Schmitt³

2015

ACES

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The most important physico-chemical characteristics describing detergent micelles are the CMC value and the aggregation number. These parameters depend critically on external conditions such as pH, ionic strength, buffer composition or temperature. However, the influence of these conditions on CMC or aggregation number is a priori not to predict, but the most important parameter. This holds especially for the aggregation number. Here, we present a simple, reliable and fast method for the analysis of detergent containing buffer systems. It enables the determination of CMC values and aggregation numbers of detergents as well as detergent mixtures in a 96well plate standard device relying on steady state fluorescence and fluorescence quenching. To prove the general applicability of our approach, we analyzed DDM as a prime example of a detergent and mixtures of DDM with six other detergents at different ratios to demonstrate the potential of our method.

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“…[55,71,72] Kalyanasundaram et al [73] demonstrated that the quenching, while being ad iffusional quenching mechanism that operates among pyridinium ], obtained by using pyrene.…”

Section: Introductionmentioning

confidence: 97%

Synthesis, Physicochemical Characterization, and Interaction with DNA of Long‐Alkyl‐Chain Gemini Pyridinium Surfactants

et al. 2015

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Pyridinium gemini surfactants with hexadecyl chains linked to nitrogen atoms and a tuned aliphatic spacer that bridges the two pyridinium polar heads in 2,2′‐positions have been synthesized and characterized. A multitechnique approach allowed us to study the aggregation behavior, using conductivity, surface tension, and fluorescence. Graphs of the specific conductivity (κ) versus the surfactant molar concentration (C), and graphs of the molar conductivity (Λ) versus C0.5 suggest pre‐aggregation phenomena of these amphiphiles at very low concentration. The trends of Amin as a function of the spacer length confirm the hypothesis of a conformational change of the molecule with four methylene groups as spacer owing to stacking interactions between the two pyridinium rings mediated by the counterion. Moreover, the trends of Amin and counterion binding (β) suggest that the spacer must be longer than eight carbon atoms to fold efficiently toward the micellar core. The opportunity to tune the surfactant structure and aggregation properties make those surfactants—particularly the long‐chain ones for which the DNA complexing ability was shown by means of atomic force microscopy (AFM) imaging—desirable candidates for gene‐delivery experiments.

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Fluorescence quenching in micellar solutions and its application to the determination of aggregation numbers

Cited by 252 publications

References 3 publications

Mixed Micellization of Dimeric (Gemini) Surfactants and Conventional Surfactants

Mixed Micellization of Dimeric (Gemini) Surfactants and Conventional Surfactants

Analyzing the Physico-Chemical Parameters of Detergents and Detergent Mixtures

Synthesis, Physicochemical Characterization, and Interaction with DNA of Long‐Alkyl‐Chain Gemini Pyridinium Surfactants

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