Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecules

Dong, Haifeng; Gao, Wenchao; Yan, Feng; Ji, Hanxu; Ju, Huangxian

doi:10.1021/ac100852z

Cited by 746 publications

(481 citation statements)

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“…(24). This effect has also been verified experimentally by Dong et al,32 where upon integrating a molecular beacon to a CdTe QD, it was found that the fluorescence quenching due to graphene is modified. We also note that at certain values of probe detuning, say for example, δ 2 ≈ ±1.5 μeV, the sensitivity of the energy transfer rate to the change in dielectric constant is quite high.…”

Section: Resultssupporting

confidence: 66%

“…21,22,[26][27][28][29] Recently, experimental research on graphene has been extended to the fabrication and study of QD-graphene nanostructures. [30][31][32][33][34] For example, a CdS QD-graphene hybrid system has been synthesized by Cao et al, 30 in which a picosecond ultrafast electron transfer process from the excited QD to the graphene matrix was observed using time-resolved fluorescence spectroscopy. Chen et al 31 have fabricated CdSe/ZnS QDs in contact with single-and few-layer graphene sheets.…”

Section: Introductionmentioning

confidence: 99%

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Dipole-dipole interaction between a quantum dot and a graphene nanodisk

et al. 2012

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We study theoretically the dipole-dipole interaction and energy transfer in a hybrid system consisting of a quantum dot and graphene nanodisk embedded in a nonlinear photonic crystal. In our model, a probe laser field is applied to measure the energy transfer between the quantum dot and graphene nanodisk, while a control field manipulates the energy transfer process. These fields create excitons in the quantum dot and surface plasmon polaritons in the graphene nanodisk which interact via the dipole-dipole interaction. Here, the nonlinear photonic crystal acts as a tunable photonic reservoir for the quantum dot, and is used to control the energy transfer. We have found that the spectrum of power absorption in the quantum dot has two peaks due to the creation of two dressed excitons in the presence of the dipole-dipole interaction. The energy transfer rate spectrum of the graphene nanodisk also has two peaks due to the absorption of these two dressed excitons. Additionally, energy transfer between the quantum dot and the graphene nanodisk can be switched on and off by applying a pump laser to the photonic crystal or by adjusting the strength of the dipole-dipole interaction. We show that the intensity and frequencies of the peaks in the energy transfer rate spectra can be modified by changing the number of graphene monolayers in the nanodisk or the separation between the quantum dot and graphene. Our results agree with existing experiments on a qualitative basis. The principle of our system can be employed to fabricate nanobiosensors, optical nanoswitches, and energy transfer devices.

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Section: Resultssupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Dipole-dipole interaction between a quantum dot and a graphene nanodisk

et al. 2012

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“…Since NCs are naturally conjugated to DNA after the synthesis, a molecular beacon setup is an obvious method of using these NCs as a fluorophore substituent. Similar applications have already been demonstrated using semiconductor quantum dots [86,87], although it needs to be noted that it is quite difficult to conjugate DNA to an as-synthesized quantum dot. Since DNA is already a part of the NC structure, it is unnecessary to perform additional conjugation reactions.…”

Section: Molecular Beaconsmentioning

confidence: 75%

“…Graphene oxide (GO) is a general quencher for a diverse range of fluorophores, including semiconductor quantum dots [87,89,90]. DNA can be adsorbed by GO and also desorb by adding its cDNA to form a duplex [91].…”

Section: Molecular Beaconsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

197

141

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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“…GO strongly adsorbs nonstructured single-stranded (ss-DNA), while adsorption of well-folded or double-stranded (ds) DNA is disfavored. Combined with its superior fluorescence quenching ability, a number of sensors have been prepared to detect metal ions, 31,32 small molecules, 13,33,34 proteins, 35 DNA, [36][37][38][39][40][41] and cells. 42 Most of these sensors were designed using a scheme shown in Figure 1A.…”

Section: Introductionmentioning

confidence: 99%

Molecular Beacon Lighting up on Graphene Oxide

2012

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A molecular beacon (MB) is comprised of a fluorophore and a quencher linked by a DNA hairpin. MBs have been widely used for homogeneous DNA detection. In addition to molecular quenchers, many nanomaterials such as graphene oxide (GO) also possess excellent quenching efficiency. Most reported fluorescent sensors relied on DNA probes physisorbed by GO, which may suffer from non-specific probe displacement and false positive signal. In this work, we report the preparation and characterization of a MB using graphene oxide (GO) as quencher, where an amino and FAM (6carboxyfluorescein) dual labeled DNA was covalently attached to GO via an amide linkage. A major challenge was to remove noncovalently attached probes due to strong DNA adsorption by GO. While DNA desorption was favored at low salt, high pH, high temperature, and by using organic solvents, the complementary DNA was required to achieve complete desorption of non-covalently linked DNA probes. The DNA adsorption energy was measured using isothermal titration calorimetry, revealing the heterogeneous nature of GO. The covalent probe has a detection limit of 2.2 nM using a sample volume of 0.05 mL. With 2 mL sample, the detection limit can reach 150 pM. The covalent probe is highly resistant to non-specific probe displacement and will find applications in serum and cellular samples where high probe stability is demanded.

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Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecules

Cited by 746 publications

References 50 publications

Dipole-dipole interaction between a quantum dot and a graphene nanodisk

Dipole-dipole interaction between a quantum dot and a graphene nanodisk

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Molecular Beacon Lighting up on Graphene Oxide

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