Fluorescence spectroscopic characterisation of algal organic matter: towards improved<i>in situ</i>fluorometer development

Khan, Sara Imran; Zamyadi, Arash; Rao, N.R.H.; Li, Xiang; Stuetz, Richard M.; Henderson, Rita K.

doi:10.1039/c8ew00731d

Cited by 19 publications

(7 citation statements)

References 89 publications

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“…The data was further processed using methods described previously. 8,46 Briefly, data was corrected for inner filter effect using correction factors obtained from UV absorbance. All samples were also individually tested for inner filtering effects due to high sample concentration by preparing a calibration curve and diluting samples before measurement accordingly.…”

Section: Methodsmentioning

confidence: 99%

Considerations of the limitations of commonly applied characterisation methods in understanding protein-driven irreversible fouling

Mustafa

Rao

Henderson

et al. 2022

Environ. Sci.: Water Res. Technol.

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Section: Methodsmentioning

confidence: 99%

Considerations of the limitations of commonly applied characterisation methods in understanding protein-driven irreversible fouling

Mustafa

Rao

Henderson

et al. 2022

Environ. Sci.: Water Res. Technol.

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“…The physiological window of beneficial HS doses is narrow, and inhibitory doses must be avoided. Microalgae release into their cultivation media various organic compounds with various molecular masses, including aromatic amino acids such as tryptophan and tyrosine, heterocyclic pigments such as biopterin, proteins, etc., that generate humic-like substances, algal organic matter (AOM) [ 182 ]. This dissolved organic matter that results from microalgae organisms is also called humic matter due to its aromaticity (π-π systems) related to significant fluorescence [ 183 , 184 ].…”

Section: Humic Substances As Microalgae Biostimulantsmentioning

confidence: 99%

Humic Substances as Microalgal Biostimulants—Implications for Microalgal Biotechnology

Popa

Lupu

Constantinescu-Aruxandei

et al. 2022

Marine Drugs

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Humic substances (HS) act as biostimulants for terrestrial photosynthetic organisms. Their effects on plants are related to specific HS features: pH and redox buffering activities, (pseudo)emulsifying and surfactant characteristics, capacity to bind metallic ions and to encapsulate labile hydrophobic molecules, ability to adsorb to the wall structures of cells. The specific properties of HS result from the complexity of their supramolecular structure. This structure is more dynamic in aqueous solutions/suspensions than in soil, which enhances the specific characteristics of HS. Therefore, HS effects on microalgae are more pronounced than on terrestrial plants. The reported HS effects on microalgae include increased ionic nutrient availability, improved protection against abiotic stress, including against various chemical pollutants and ionic species of potentially toxic elements, higher accumulation of value-added ingredients, and enhanced bio-flocculation. These HS effects are similar to those on terrestrial plants and could be considered microalgal biostimulant effects. Such biostimulant effects are underutilized in current microalgal biotechnology. This review presents knowledge related to interactions between microalgae and humic substances and analyzes the potential of HS to enhance the productivity and profitability of microalgal biotechnology.

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“…Fluorescence spectral analysis is extensively used to measure multiple fluorescent compounds in sample solutions in diverse fields ranging from biomedical applications to environmental monitoring. − Förster resonance energy transfer or fluorescence resonance energy transfer (FRET) requires spectral measurements to quantify the relative intensity between donor and acceptor fluorophores and enables the real-time analysis of molecular structure changes and interactions by accurately measuring molecular proximity. − Multiple fluorescence colors of quantum dots need to be quantified for multiplexed assays that exploit the unique optical characteristics of fluorescent nanocrystals, such as simultaneous excitation for multiple fluorescence colors and size-tunable emission wavelengths. − Fluorescence spectroscopic characterization of algal and cyanobacterial cells provides a powerful method to discriminate problematic species and predict algal blooms. − Fluorescence spectroscopy is a highly versatile and widely used technique for these applications; however, commercial spectrometers remain bulky, delicate, and expensive, which limits their use to well-equipped laboratories.…”

Section: Introductionmentioning

confidence: 99%

Open-Source Fluorescence Spectrometer for Noncontact Scientific Research and Education

et al. 2021

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Transforming fluorescence spectrometers into costeffective, portable devices provides the potential for field-based applications in biological, environmental, and clinical research and education. However, the majority of developed spectroscopic technologies continue to require heavy, expensive equipment and trained personnel for operation or do not support multispectral analysis, thereby restricting their use in resource-limited environments. Herein, we report a wireless, portable, cost-effective, opensource fluorescence spectrometer (OpenFS) developed by compactly assembling optical and electronic elements in a 3Dprinted housing. OpenFS outputs an accurate emission spectrum over a wide range of wavelengths and demonstrates greater sensitivity for fluorescence quantification compared to a conventional fluorometer. We demonstrate the functionality of OpenFS as a fluorescence resonance energy transfer (FRET)-based DNA sensor by detecting target DNA molecules with FRET efficiency and prove its utility as an Internet of Things device by performing wireless measurements and spectral analysis on a smartphone with a custom-developed Android application. This portable open-source spectrometer can lead to new opportunities in research and educational fields where fluorescence spectroscopy has not been available because of its cost and size and provides the potential for the development of mobile diagnostics platforms.

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Fluorescence spectroscopic characterisation of algal organic matter: towards improvedin situfluorometer development

Abstract: Fluorescence based characterisation of the algal organic matter, when combined with cell pigmentation measurements, may improve the specificity and robustness of online fluorometers.

Cited by 19 publications

References 89 publications

Considerations of the limitations of commonly applied characterisation methods in understanding protein-driven irreversible fouling

Considerations of the limitations of commonly applied characterisation methods in understanding protein-driven irreversible fouling

Humic Substances as Microalgal Biostimulants—Implications for Microalgal Biotechnology

Open-Source Fluorescence Spectrometer for Noncontact Scientific Research and Education

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