Fluorescence study on rat epithelial cells and liposomes exposed to aromatic nitroxides

Gabbianelli, Rosita; Falcioni, Giancarlo; Lupidi, Giulio; Greci, Lucedio; Damiani, Elisabetta

doi:10.1016/j.cca.2004.03.006

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The results of the changes in anisotropy are presented in Figure 10. The control value (0.117) of anisotropy in PC membrane at 25 °C is in a good agreement with results obtained by other authors (51). The largest changes in anisotropy are caused by the mixture Que/DPhT.…”

Section: Thesupporting

confidence: 91%

Antioxidative Effect of Quercetin and Its Equimolar Mixtures with Phenyltin Compounds on Liposome Membranes

Gabrielska¹,

Soczyńska-Kordala²,

Hładyszowski³

et al. 2006

J. Agric. Food Chem.

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Our earlier studies have shown that the compounds diphenyltin dichloride (DPhT) and triphenyltin chloride (TPhT) in the presence of UVC radiation enhanced the degree of phosphatidylcholine liposome membrane oxidation (J. Agric. Food Chem. 2005, 53, 76-83). The prooxidative behavior of the compounds has now been confirmed with the electron paramagnetic resonance method, which proved the possibility that the studied compounds can exist in free radical forms. The present work investigates the possibility of the protective action of quercetin on phosphatidylcholine liposome membranes exposed to the prooxidative action of DPhT and TPhT induced by UV radiation (lambda = 253.7 nm). The concentrations of quercetin and its equimolar mixtures with DPhT and TPhT were determined (and compared with well-known antioxidants as standards-trolox and butylated hydroxytoluene, also in the presence of phenyltins) as those that induce 50% inhibition in oxidation of liposomes radiated with UV. They are 5.1 +/- 0.10, 2.9 +/- 0.12, and 1.9 +/- 0.08 microM (differences between the values are statistically significant), constituting the following sequence of antioxidative activity: quercetin:TPhT > quercetin:DPhT > quercetin. This relation is confirmed by the results on the antiradical ability of quercetin and its mixtures with DPhT and TPhT toward the free radical 1,1-diphenyl-2-pricrylhydrazil. Similar sequences obtained in both studies suggest a possible mechanism of the antiradical action of the mixtures as free radical scavengers. We suggested that (i) quercetin's ability, documented by spectrophotometric, infrared attenuated total reflectance spectroscopy, (1)H NMR, and molecular modeling methods, to form complexes with phenyltins indicates a possible way of protection against the peroxidation caused by the free radical forms of phenyltins and (ii) the differentiation in the action of the quercetin/TPhT and quercetin/DPhT associates (statisticaly significant) may result from a different localization in the liposome membrane, which is indicated by the results of the fluorimetric studies.

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Section: Thesupporting

confidence: 91%

Antioxidative Effect of Quercetin and Its Equimolar Mixtures with Phenyltin Compounds on Liposome Membranes

Gabrielska¹,

Soczyńska-Kordala²,

Hładyszowski³

et al. 2006

J. Agric. Food Chem.

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“…DNA damage in lymphocytes was evaluated using the alkaline single-cell microgel electrophoresis (‘comet’ assay) [32], [33]. The comet assay was carried out under yellow light.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Activities of Extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva Medicinal Plants Collected in Cameroon

et al. 2014

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BackgroundThe vast majority of the population around the world has always used medicinal plants as first source of health care to fight infectious and non infectious diseases. Most of these medicinal plants may have scientific evidence to be considered in general practice.ObjectiveThe aim of this work was to investigate the antioxidant capacities and anti-inflammatory activities of ethanol extracts of leaves of Cassia alata, Eleusine indica, Carica papaya, Eremomastax speciosa and the stem bark of Polyscias fulva, collected in Cameroon.MethodsChemiluminescence was used to analyze the antioxidant activities of plant extracts against hydrogen peroxide or superoxide anion. Comet assays were used to analyze the protection against antioxidant-induced DNA damage induced in white blood cells after treating with hydrogen peroxide. Flow cytometry was used to measure γδ T cells proliferation and anti-inflammatory activity of γδ T cells and of immature dendritic cells (imDC) in the presence of different concentrations of plant extracts.ResultsEthanol extracts showed strong antioxidant properties against both hydrogen peroxide and superoxide anion. Cassia alata showed the highest antioxidant activity. The effect of plant extracts on γδ T cells and imDC was evidenced by the dose dependent reduction in TNF-α production in the presence of Cassia alata, Carica papaya, Eremomastax speciosa Eleusine indica, and Polyscias fulva. γδ T cells proliferation was affected to the greatest extent by Polyscias fulva.ConclusionThese results clearly show the antioxidant capacity and anti-inflammatory activities of plant extracts collected in Cameroon. These properties of leaves and stem bark extracts may contribute to the value for these plants in traditional medicine and in general medical practice.

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Protective effect of ethyl pyruvate on msP rat leukocytes damaged by alcohol intake

Fedeli¹,

Falcioni²,

Olek³

et al. 2007

J of Applied Toxicology

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Alcohol consumption for long periods negatively influences physiological functions of many cells, and leads to organ damage. Reactive oxygen and nitrogen species produced by ethanol metabolism cause adverse effects that might be alleviated by simultaneous treatment with various antioxidants. Here, the ability of ethyl pyruvate (EP) to reduce ethanol-induced oxidative stress was evaluated. Chemiluminescence studies show that EP has a higher capacity than pyruvate to scavenge hydrogen peroxide and superoxide anions. In order to evaluate whether EP can exert a protective effect against ethanol, rats were offered 10% ethanol in drinking burettes, containing or not different concentrations of EP (0.3%, 1% and 3%). The comet assay was employed to quantify the alcohol-induced DNA damage in rat lymphocytes. This test is a promising tool for the estimation of DNA damage at the single cell level. A significant protective effect of EP was observed in rat groups treated with this antioxidant, compared with those drinking only ethanol. Since EP has been shown to decrease the expression of numerous pro-inflammatory mediators, the monocyte respiratory burst was evaluated. The activation of monocyte NADPH oxidase by phorbol esters (PMA) showed that superoxide anion production was higher in the ethanol group than in the control group. The presence of EP considerably reduced superoxide anion production. In conclusion, hypotheses on possible mechanisms of action of EP on rat white blood cells are proposed.

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Fluorescence study on rat epithelial cells and liposomes exposed to aromatic nitroxides

Cited by 4 publications

References 39 publications

Antioxidative Effect of Quercetin and Its Equimolar Mixtures with Phenyltin Compounds on Liposome Membranes

Antioxidative Effect of Quercetin and Its Equimolar Mixtures with Phenyltin Compounds on Liposome Membranes

Antioxidant and Anti-Inflammatory Activities of Extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva Medicinal Plants Collected in Cameroon

Protective effect of ethyl pyruvate on msP rat leukocytes damaged by alcohol intake

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