Fluorescence turn-on probe for homocysteine and cysteine in water

Lee, Kyung Sik; Kim, Tae Ki; Lee, Jong Ho; Kim, Hae-Jo; Hong, Jong Ha

doi:10.1039/b814581d

Cited by 233 publications

(44 citation statements)

References 40 publications

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“…A wide variety of sensing mechanisms have been developed for molecular fluorescent thiol probes Chen et al, 2010a,b;Lin et al, 2010;Ruan et al, 2010;Yi et al, 2009;Amoroso et al, 2008;Lee et al, 2008;Tang et al, 2009;Zhu et al, 2010;Zhang et al, 2007Zhang et al, , 2010a. One of the methods is to attach electrophilic group to the fluorophores (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Alternatively dyads of fluorophore/maleimides were used as thiol probes (Matsumoto et al, 2007;Weh et al, 2007;Maeda et al, 2006). Fluorophores containing formyl (-CHO) group were also used as thiol probes, which are based on tuning the intramolecular charge transfer (ICT) effect by addition with thiols (Tang et al, 2007;Tanaka et al, 2004;Zhang et al, 2006;Lee et al, 2008;Chen et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

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Styryl-BODIPY based red-emitting fluorescent OFF–ON molecular probe for specific detection of cysteine

Shao

Guo

et al. 2011

Biosensors and Bioelectronics

150

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Styryl-BODIPY based red-emitting fluorescent OFF–ON molecular probe for specific detection of cysteine

Shao

Guo

et al. 2011

Biosensors and Bioelectronics

150

View full text Add to dashboard Cite

“…Several methods based on chromatography, electrophoresis, and fluorescent technology for biothiol detection have been reported, especially nanomaterial-based methods much facilitated by the development of the underlying nanotechnology (Lee et al, 2008;Hong et al, 2009;Yi et al, 2009;Shiu et al, 2010;Liu et al, 2014;Zhang et al, 2014;Zhu et al, 2014). Each method has its own advantage; however, pretreatments of the materials or expensive equipments are often necessary to obtain a good performance.…”

Section: Introductionmentioning

confidence: 99%

Detection of biological thiols based on a colorimetric method

Sun

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.

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“…Besides synthetic organic probes [23][24][25][26], metal nanoparticles, such GNPs and gold nanorods, were involved in sensing thiols, due to their easy synthesis and biocompatibility [27][28][29]. The presence of thiols reacted and immobilized on the surface of nanoparticles and induced them to aggregate, turning out a colour change.…”

Section: Detection Of Thiolsmentioning

confidence: 99%

DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols

Jin

Ouyang

et al. 2011

Sci. China Chem.

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To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and attracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles (AgNPs) were obtained via reduction of Ag + on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the template-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were employed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio (S/B), the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 μM to 302 μM with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%. silver nanoparticles, DNA template-synthesized, thiols, fluorescent sensing

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Fluorescence turn-on probe for homocysteine and cysteine in water

Abstract: A simple fluorescent probe based on an ortho-hydroxy aldehyde-functionalized coumarin showed selective responses to homocysteine and cysteine by fluorescence turn-on.

Cited by 233 publications

References 40 publications

Styryl-BODIPY based red-emitting fluorescent OFF–ON molecular probe for specific detection of cysteine

Styryl-BODIPY based red-emitting fluorescent OFF–ON molecular probe for specific detection of cysteine

Detection of biological thiols based on a colorimetric method

DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols

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