Fluorescent aptasensor for detection of four tetracycline veterinary drugs in milk based on catalytic hairpin assembly reaction and displacement of G-quadruplex

Zhou, Chen; Zou, Haimin; Sun, Chengjun; Ren, Dongxia; Xiong, Wei; Li, Yongxin

doi:10.1007/s00216-018-0981-2

Cited by 25 publications

(8 citation statements)

References 48 publications

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“…As for the HCR, analytes other than nucleic acids can be detected by coupling the CHA scheme to aptamer target recognition. CHA has also been used in more unconventional applications (e.g., for the detection of uracil-DNA glycosylase activity and in the context of food safety). , For instance, CHA has been proposed for the detection of tetracycline-based antibiotics in milk samples . In this application, one of the CHA hairpins (H1, cf.…”

Section: Applications In Sensing Diagnostics and Therapeuticsmentioning

confidence: 99%

Principles and Applications of Nucleic Acid Strand Displacement Reactions

2019

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Dynamic DNA nanotechnology, a subfield of DNA nanotechnology, is concerned with the study and application of nucleic acid strand-displacement reactions. Strand-displacement reactions generally proceed by three-way or four-way branch migration and initially were investigated for their relevance to genetic recombination. Through the use of toeholds, which are single-stranded segments of DNA to which an invader strand can bind to initiate branch migration, the rate with which strand displacement reactions proceed can be varied by more than 6 orders of magnitude. In addition, the use of toeholds enables the construction of enzyme-free DNA reaction networks exhibiting complex dynamical behavior. A demonstration of this was provided in the year 2000, in which strand displacement reactions were employed to drive a DNAbased nanomachine et al. Nature 2000, 406, 605−608). Since then, toeholdmediated strand displacement reactions have been used with ever increasing sophistication and the field of dynamic DNA nanotechnology has grown exponentially. Besides molecular machines, the field has produced enzyme-free catalytic systems, all DNA chemical oscillators and the most complex molecular computers yet devised. Enzyme-free catalytic systems can function as chemical amplifiers and as such have received considerable attention for sensing and detection applications in chemistry and medical diagnostics. Strand-displacement reactions have been combined with other enzymatically driven processes and have also been employed within living cells (Groves, B.; et al. Nat. Nanotechnol. 2015, 11, 287−294). Strand-displacement principles have also been applied in synthetic biology to enable artificial gene regulation and computation in bacteria. Given the enormous progress of dynamic DNA nanotechnology over the past years, the field now seems poised for practical application.

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Section: Applications In Sensing Diagnostics and Therapeuticsmentioning

confidence: 99%

Principles and Applications of Nucleic Acid Strand Displacement Reactions

2019

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“…It can be seen that the presented protocol provided lower LOD values, the possible reason could be that MIP‐SPME fiber possessed high specificity for the targeted TCs. Additionally, the recovery and RSD of this method was comparable to other methods [11,24, 36–38], demonstrating that the proposed method is a novel, sensitive, repeatable and ecofriendly method for the analysis of TCs residues from complex samples.…”

Section: Resultsmentioning

confidence: 60%

Preparation of hydrophilic molecularly imprinted solid‐phase microextraction fiber for the selective removal and extraction of trace tetracyclines residues in animal derived foods

Lü

et al. 2020

J of Separation Science

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The present work reported a novel hydrophilic and selective solid-phase microextraction fiber by improved multiple co-polymerization method immobilization of tetracycline molecularly imprinted polymer on a stainless steel wire and directly coupled with high-performance liquid chromatography for sensitive determination of trace tetracyclines residues in animal derived foods. The developed molecularly imprinted polymer coated solid-phase microextraction fibers were characterized through scanning electron microscopy, Fourier transfer infrared spectroscopy, thermogravimetric analysis, and adsorption experiments, the fiber with cross-linked and porous structure was observed and high thermal and chemical stability. The maximum adsorption capacity of this fiber with good selectivity reached 2.35 µg/mg in aqueous matrices, and showed good repeatability (relative standard deviation ≤ 6.6%, n = 5) and satisfying reproducibility between fiber to fiber (relative standard deviation ≤ 7.8%, n = 5). Under the optimized solid-phase microextraction conditions, satisfactory linearity (5-1000 µg/L) and detection limits (0.38-0.72 µg/kg, S/N = 3) for all the tetracyclines were obtained. The practicality of this method was proved by adding tetracycline, oxytetracycline at three levels to milk, chicken, and fish samples with good recoveries of 77.3-104.4%. K E Y W O R D Shigh-performance liquid chromatography, hydrophilic molecularly imprinted polymers, solid-phase microextraction, tetracyclines 2172

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“…Truncated broadly specific aptamers can be flexibly applied in the design of aptasensors. Zhou et al [ 76 ] utilized the 8-mer aptamer to design a fluorescent aptasensing approach based on catalytic hairpin assembly and displacement of the G-quadruplex DNA. The sensitive detection of the four TCs in milk was achieved, with an LOD of 4.6 μg/L.…”

Section: Rapid Multi-residue Detection Methods Based On Different mentioning

confidence: 99%

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

et al. 2020

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The excessive use or abuse of pesticides and veterinary drugs leads to residues in food, which can threaten human health. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of pesticide and veterinary drug residues, which can be applied as screening techniques for food safety monitoring and detection. Recent developments related to rapid multi-residue detection methods for pesticide and veterinary drug residues are reviewed herein. Methods based on different recognition elements or the inherent characteristics of pesticides and veterinary drugs are described in detail. The preparation and application of three broadly specific recognition elements—antibodies, aptamers, and molecular imprinted polymers—are summarized. Furthermore, enzymatic inhibition-based sensors, near-infrared spectroscopy, and SERS spectroscopy based on the inherent characteristics are also discussed. The aim of this review is to provide a useful reference for the further development of rapid multi-analyte analysis of pesticide and veterinary drug residues.

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Fluorescent aptasensor for detection of four tetracycline veterinary drugs in milk based on catalytic hairpin assembly reaction and displacement of G-quadruplex

Cited by 25 publications

References 48 publications

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Preparation of hydrophilic molecularly imprinted solid‐phase microextraction fiber for the selective removal and extraction of trace tetracyclines residues in animal derived foods

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

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