Fluorescent carbon nanoparticles derived from natural materials of mango fruit for bio-imaging probes

Abstract

Water soluble fluorescent carbon nanoparticles (FCP) obtained from a single natural source, mango fruit, were developed as unique materials for non-toxic bio-imaging with different colors and particle sizes. The prepared FCPs showed blue (FCP-B), green (FCP-G) and yellow (FCP-Y) fluorescence, derived by the controlled carbonization method. The FCPs demonstrated hydrodynamic diameters of 5-15 nm, holding great promise for clinical applications. The biocompatible FCPs demonstrated great potential in biological fields through the results of in vitro imaging and in vivo biodistribution. Using intravenously administered FCPs with different colored particles, we precisely defined the clearance and biodistribution, showing rapid and efficient urinary excretion for safe elimination from the body. These findings therefore suggest the promising possibility of using natural sources for producing fluorescent materials.

Phenolic condensation and facilitation of fluorescent carbon dot formation: a mechanism study

“…, carbon dots) are promising fluorescent materials, 1–3 which have been applied to various applications, such as bio-imaging tools, 4 photodynamic therapy, 5 chemical sensors, 6 and optoelectronic devices. 7 Recent studies have shown that carbon dots can be prepared from abundant vegetables and fruits such as birch kraft pulp, 8 mango fruit, 9 sweet pepper, 10 grass, 11 and pomelo peel. 12 These natural polymers are composed primarily of polysaccharides, which are rapidly condensed in the presence of strong acids to form carbon dots with a certain degree of homogeneity in their optical properties and dimensions.…”

Reversible Fluorescence Probe Based on N-Doped Carbon Dots for the Determination of Mercury Ion and Glutathione in Waters and Living Cells

“…S1D, Supporting Information) shows two peaks at 398.5 and 399.9 eV that indicate that nitrogen existed mostly in the form of O=C-N and C-N groups. 26,27 All above results indicated that the surface of the as-prepared N-CDs was passivated by multiple oxygenated and nitrous groups. The UV-vis absorption and PL spectra of the N-CDs are shown in Fig.…”

Preparation of carbon dot‐based ratiometric fluorescent probes for cellular imaging from Curcuma longa

“…Quinine sulfate (QY 55 (%) at 354 nm excitation) was used as a reference standard to measure quantum yield, adapted from a published report. [6] A particle size histogram was obtained using an ELS-Z size analyzer from Otsuka Electronics Corporation (Osaka, Japan).…”

“…[5] However, for organic compounds, FNPs from condensation reactions as well as carbonization and dehydration systems are the best choice due to their simple manufacturing method. [6] They possess novel properties including water solubility, high stability, biocompatibility, and high optical absorption efficiency with tunable emissions through efficient size dependent excitation. [7][8][9][10] Some reports cite problems when using FNPs such as instability, persistence in the body, and expense of availability.…”

“…For example, the FNPs from mango (Mangifera indica L.) demonstrate great potential for use in cell-imaging through a controlled carbonization method, which can produce blue, green, and yellow colored emissions at certain excitation wavelengths for in vitro imaging and in vivo biodistribution. [6] In contrast to polymer dots that can self-passivate because of their outer shell, carbonized natural sources require surface passivation to produce the expected quantum yield and emission intensity. [10] Curcuma longa is a major compound obtained from turmeric, a Zingiberaceae family member, and is an active polyphenol extensively used in pharmacological applications for its anticancer, anti-inflammatory, anti-oxidant, anti-aging, and antibacterial behaviors.…”