Fluorescent Cyclic Voltammetry of Immobilized Azurin: Direct Observation of Thermodynamic and Kinetic Heterogeneity

Salverda, Jante M.; Patil, Amol V.; Mizzon, Giulia; Кузнецова, С. А.; Zauner, Gerhild; Akkiliç, Namık; Canters, Gerard W.; Davis, Jason J.; Heering, Hendrik A.; Aartsma, Thijs J.

doi:10.1002/ange.201001298

Cited by 24 publications

(30 citation statements)

References 45 publications

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“…In the first case, the fluorophore is linked to a redox moiety that acts as fluorescence switching through energy 9,10 or electron transfer mechanisms [11][12][13][14] . In the latter, electrochemical fluorescence switching is enabled on direct reduction or oxidation of the fluorophore (either small molecules or conjugated polymers) [15][16][17][18] . Electrofluorochromism is usually coupled to electrochromism of the redox unit in dyads or of the electroactive fluorophore.…”

mentioning

confidence: 99%

Electrofluorochromism in π-conjugated ionic liquid crystals

et al. 2014

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Materials in which photoluminescence is modulated by redox processes are known as electrofluorochromic. Intrinsically switchable fluorophores, incorporating both redox and fluorescent moieties, could be ideal electrofluorochromic materials if they possess high fluorescence quantum yields in at least one of their redox states. Fluorescent liquid crystals with redox active centres could combine the above requirements with the advantage to work in bulk anisotropic phases. However, electrofluorochromic liquid crystals have not been reported yet because their synthesis is challenging due to aggregation-caused fluorescent quenching. Here we show the first examples of electrofluorochromic p-conjugated ionic liquid crystals based on thienoviologens. These ordered materials, combining ionic and electronic functions, are highly fluorescence in the bulk state (quantum yield460%). Their direct electrochemical reduction leads to fast and reversible bulk electrofluorochromic response in both columnar and smectic phases allowing for fluorescence intensity modulation and colour tuning.

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confidence: 99%

Electrofluorochromism in π-conjugated ionic liquid crystals

et al. 2014

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“…[19][20][21][22][23] The application of this scheme to the study of the electrochemical behavior of electron-transfer proteins has recently been reported. [24] In earlier studies on the green NiR (gNiR; Figure S4 in the Supporting Information) from Alcaligenes faecalis-S6, the intensity distributions in fluorescence time traces of single molecules during turnover were analyzed by means of an autocorrelation analysis. [22] Herein we have used the blue NiR (bNiR; Figure S4 in the Supporting Information) from A. xylosoxidans, the T1 copper site of which exhibits a more pronounced absorbance around 600 nm when oxidized.…”

mentioning

confidence: 99%

Fluorescence Lifetime Analysis of Nitrite Reductase from Alcaligenes xylosoxidans at the Single‐Molecule Level Reveals the Enzyme Mechanism

Tabares

Kostrz

Elmalk

et al. 2011

Chemistry A European J

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“…alkane headgroup) alkanethiols are thought to facilitate direct electron transfer between a gold electrode and azurin because the protein has a patch of hydrophobic surface residues proximal to the redox‐active copper center . The stability of azurin on such alkanethiol SAMs has been put to particularly good use in the quantification of kinetic and thermodynamic dispersion, through the coupling of fluorescence monitoring of the copper redox state with electrochemical control of the redox potential …”

Section: Common Electrode Functionalization Strategies To Promote Elementioning

confidence: 99%

Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

Yates

Fascione

Parkin

2018

Chemistry A European J

109

116

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The immobilization of redox proteins or enzymes onto conductive surfaces has application in the analysis of biological processes, the fabrication of biosensors, and in the development of green technologies and biochemical synthetic approaches. This review evaluates the methods through which redox proteins can be attached to electrode surfaces in a “wired” configuration, that is, one that facilitates direct electron transfer. The feasibility of simple electroactive adsorption onto a range of electrode surfaces is illustrated, with a highlight on the recent advances that have been achieved in biotechnological device construction using carbon materials and metal oxides. The covalent crosslinking strategies commonly used for the modification and biofunctionalization of electrode surfaces are also evaluated. Recent innovations in harnessing chemical biology methods for electrically wiring redox biology to surfaces are emphasized.

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Fluorescent Cyclic Voltammetry of Immobilized Azurin: Direct Observation of Thermodynamic and Kinetic Heterogeneity

Cited by 24 publications

References 45 publications

Electrofluorochromism in π-conjugated ionic liquid crystals

Electrofluorochromism in π-conjugated ionic liquid crystals

Fluorescence Lifetime Analysis of Nitrite Reductase from Alcaligenes xylosoxidans at the Single‐Molecule Level Reveals the Enzyme Mechanism

Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

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