Fluorescent Ensemble Based on Bispyrene Fluorophore and Surfactant Assemblies: Sensing and Discriminating Proteins in Aqueous Solution

Abstract

A particular bispyrene fluorophore (1) with two pyrene moieties covalently linked via a hydrophilic spacer was synthesized. Fluorescence measurements reveal that the fluorescence emission of 1 could be well modulated by a cationic surfactant, dodecyltrimethylammonium bromide (DTAB). Protein sensing studies illustrate that the selected ensemble based on 1/DTAB assemblies exhibits ratiometric responses to nonmetalloproteins and turn-off responses to metalloproteins, which can be used to differentiate the two types of proteins. Moreover, negatively charged nonmetalloproteins can be discriminated from the positively charged ones according to the difference in ratiometric responses. Fluorescence sensing studies with control bispyrenes indicate that the polarity of the spacer connecting two pyrene moieties plays an important role in locating bispyrene fluorophore in DTAB assemblies, which further influences its sensing behaviors to noncovalent interacting proteins. This study sheds light on the influence of the probe structure on the sensing performance of a fluorescent ensemble based on probe and surfactant assemblies.

Supramolecular Dye Aggregate Assembly Enables Ratiometric Detection and Discrimination of Lysine and Arginine in Aqueous Solution

“… 52 However, the discrimination ability is highly dependent on the number of employed sensor elements, and usually in the case of fluorescent sensor arrays, different fluorophores are commonly employed to provide the cross-reactivity that leads to the complexity of data collection which requires scanning the emission spectra of all of the sensor elements or may even require different excitation wavelengths for each of the sensor element. 53 However, in the present case, we realized that interestingly arginine shows a distinct fluorescence behavior than lysine, when compared at the identical concentration. For example, arginine displays a more prominent emission intensity at blue-shifted wavelength when compared with lysine ( Figures 6 A and S6, Supporting Information ).…”

Fluorescent binary ensemble with pattern recognition ability for identifying multiple metalloproteins with applications in serum and urine

“…23 As a different strategy, our group has made efforts using surfactant assemblies to encapsulate and modulate uorophores to develop sensitive and discriminative sensors for proteins. [24][25][26] In our rst try, we found that cationic surfactant DTAB assemblies could well modulate the uorescence emission of a cholesterol-modied pyrene-based uorophore that was highly aggregated in aqueous solution. This modulation effect could be further used to sense the protein-induced assembling variation of surfactants and realized the selective detection of two negatively charged nonmetalloproteins.…”

Aggregation Behavior and Electron‐Transfer Reaction of Dendritic Perylene Bisimide, Accompanied by the Formation of Highly Stable Anionic Radical Species

“…The use of a surfactant is ideal for stabilizing the dispersed form of a molecule . The modulating effect of sodium dodecylbenzenesulfonate (SDBS) on the aggregation of PEPBI was evaluated in aqueous solution.…”