“…[16][17][18] Therefore, the application of alternative methods is essential for further clarification of clonal constitution in myeloid disorders. In this regard, karyotype, 19,20 FISH, 15,21 or Ras mutational analysis 14 have been applied to either fractionated cell populations or in vitro progenitor colonies. In most, but not all, 14,15 instances, the results have been consistent with those derived from X-linked clonality assays.…”