Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging

Zhao, Qiang; Zhou, Xiaobo; Cao, Tianye; Zhang, Kenneth Yin; Yang, Lijuan; Liu, Shujuan; Liang, Hua; Yang, Huiran; Li, Fuyou; Huang, Wei

doi:10.1039/c4sc03062a

Cited by 218 publications

(125 citation statements)

References 54 publications

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“…For simplification, we named such kind of probes/sensors as liquid format probes/sensors. Many liquid oxygen probes/sensors by using organic compounds, macromolecules, and/or nanoparticles [21][22][23][24][25][26][27][28][29][30][31][32][33] were developed. Among these probes/sensors, although many are for intracellular oxygen measurements [22][23][24]30,31,[34][35][36][37]; some liquid sensors were demonstrated to be extracellular sensors and were used for cell respiration and mitochondrial activity measurements [21,28,29].…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)-containing graft copolymers for ratiometric extracellular oxygen sensing

Zou¹,

Pan²,

Jiang³

et al. 2017

Sensors and Actuators B: Chemical

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a b s t r a c tA graft copolymer composed of poly(ε-caprolactone) as a hydrophobic chain and poly(N-(2-hydroxypropyl)methacrylamide) as a hydrophilic segment with an internal reference probe for oxygen sensing was prepared. Highly efficient platinum(II)meso-tetra(pentafluorophenyl)porphine (PtTFPP) was used as a typical oxygen probe. Although PtTFPP was highly efficient, it is hydrophobic, limiting its direct application for oxygen sensing in biological conditions. By using the amphiphilic graft copolymer, PTTFPP was successfully incorporated into the micelles formed by this polymer to not only enable the application of PtTFPP for biosensing in medium but also retain its high efficiency. The quantum efficiency of PtTFPP in the micelle solution under nitrogen can reach as high as 0.20. The PtTFPP-containing micelles were tested for high throughput cell respiration and bacteria detection. The influence of an antibiotic, antimycin as a representative example, on cell respiration was also studied. For achieving high accurate sensing, an internal built-in reference probe with aggregation induced emission properties were also polymerized in the graft copolymer. This study demonstrated that the graft polymer approach is a good way to enable highly efficient and hydrophobic oxygen probes for biosensing.

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Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)-containing graft copolymers for ratiometric extracellular oxygen sensing

Zou¹,

Pan²,

Jiang³

et al. 2017

Sensors and Actuators B: Chemical

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“…While they were originally developed for optoelectronic devices,t he adaptation of semiconducting polymers into nanoparticles has yielded ar apidlyi ncreasing number of applicationso ft he polymer dots (Pdots)i nb iomedical theranostics. [1][2][3][4] The attractiveness of Pdots foro pticali maging, sensing, and phototherapy is due to their superior characteristics, such as large absorption coefficients, excellent photostability,b iocompatibility,a nd suitability for the efficient generation of either photoluminescence, photoacoustic waves, or reactive oxygen species (ROS). [5][6][7][8] Such versatile and extraordinary properties have motivated researchers to explore the multifunctional Pdots for biological applications.…”

mentioning

confidence: 99%

Light‐Induced PEGylation and Functionalization of Semiconductor Polymer Dots

et al. 2017

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As emiconductor polymer with side-chain oxetane groups was synthesized to form highly fluorescent, functionalized, andu ltra-stablep olymer dots (Pdots). A facile light-induced strategy covalently links functional polyethylene glycol (PEG) molecules to polymer dots while simultaneously providing functional groups for bioconjugation. The influence of photo-crosslinkable sidechains and PEG molecules on the properties and performance of Pdots was systematically investigated. The Pdots functionalized by photo-crosslinking show specific labeling and negligible non-specific binding as compared to non-functionalized Pdots. The resultsp rovide av iable strategy for nanoparticle assembly and functionalization.Semiconducting polymers have attracted considerable interest in both optoelectronic and biomedical applications over past decades. While they were originally developed for optoelectronic devices,t he adaptation of semiconducting polymers into nanoparticles has yielded ar apidlyi ncreasing number of applicationso ft he polymer dots (Pdots)i nb iomedical theranostics.[1-4] The attractiveness of Pdots foro pticali maging, sensing, and phototherapy is due to their superior characteristics, such as large absorption coefficients, excellent photostability,b iocompatibility,a nd suitability for the efficient generation of either photoluminescence, photoacoustic waves, or reactive oxygen species (ROS).[5-8] Such versatile and extraordinary properties have motivated researchers to explore the multifunctional Pdots for biological applications. Significant efforts have recently been focusedo ns ynthesizing new conjugated polymers, [9,10] developing functionalization strategies, [11,12] and designing signal transduction/amplification schemes. [13,14] The usefulness of Pdots in biological applications is strongly dependento nt he quality of the surfacef unctionalization to enables pecific recognition. Hence, many strategies have been implemented to modifyP dot surfaces with different functional molecules, for example, PEGylated lipids and amphiphilic polymers. [15,16] These functional molecules consist of hydrophobic components and hydrophilic segmentsw ith functional groups (e.g.,p olyethylene glycol,a mines, and carboxylic acids). During the Pdot formation, the hydrophobic segments are physically associated with polymer nanoparticles, while the hydrophilic chains extend partlyi nto the aqueous environment for further bioconjugation. Chiu's group developed ad irect functionalization strategy by modifying the side chain of the conjugated polymer.[17] Pdots formed from such functional polymers would directly have surface reactive groups for bioconjugation. In this strategy,the density of the side-chain functional groupss hould be well controlled to preventt he formationo farelatively loose structure, which can significantly affect nanoparticle stability and fluorescenceb rightness. However, challenges remain in the functionalization of Pdot surfaces for biomedical applications. Ideal semiconducting polymer nanoparticles ...

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“…Moreover, the ratio of the doped fluorophores is not easily controlled in the coupling reaction, which leads to an intensive energy consumption and limit their wide applications (Dmitriev et al 2015;Shi et al 2014;Zhao et al 2015). Kondrashina et al successfully developed an oxygen sensing nanoparticle through embedding oxygen probe and π-conjugated fluorophore into the positively charged Eudragits RL 100 (Kondrashina et al 2012).…”

Section: Introductionmentioning

confidence: 97%

Facile synthesis of a ratiometric oxygen nanosensor for cellular imaging

Zhang

et al. 2016

Biosensors and Bioelectronics

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Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging

Abstract: Fluorescent/phosphorescent dual-emissive conjugated polymer dots were designed and synthesized, ere used for tumor hypoxia sensing via ratiometric imaging and photoluminescence lifetime imaging.

Cited by 218 publications

References 54 publications

Poly(ε-caprolactone)-containing graft copolymers for ratiometric extracellular oxygen sensing

Poly(ε-caprolactone)-containing graft copolymers for ratiometric extracellular oxygen sensing

Light‐Induced PEGylation and Functionalization of Semiconductor Polymer Dots

Facile synthesis of a ratiometric oxygen nanosensor for cellular imaging

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