Fluorescent quantitative PCR detection ofMycobacterium tuberculosisin tissue sections from granulomatous lesions retrieved using EDTA

Abstract

This study showed that compared with special staining technologies (acid-fast and Auramine O) and molecular pathology detection, fluorescent quantitative PCR with EDTA retrieval could greatly improve the detection rate of TB/non-tuberculous mycobacteria and increase the sensitivity of the fluorescent quantitative PCR.

Description, validation, and review of a decade of experience with a laboratory-developed PCR test for detection of Mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens

“…The use of LDT PCR assays for the detection of Mtbc has been reported previously by numerous groups but, to our knowledge, none have been followed over a long period of time in order to collect actual performance data in a clinical diagnostic laboratory [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] . Some of the early LDTs were introduced as an alternative to the labor intensive and open PCR tests.…”