Fluorescent Sensor Array for Quantitative Determination of Saccharides

Pushina, Mariia; Penavic, Andrej; Farshbaf, Sepideh; Anzenbacher, Pavel

doi:10.1021/acssensors.1c01371

Cited by 35 publications

(18 citation statements)

References 62 publications

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“…The obtained data were then subjected to Student's T-test and analysed using Linear Discriminant Analysis (LDA). [38,[40][41][42] Here, the LDA was used to discriminate among different species and classify all repetitions into single cluster for each saccharide. Figure 4 shows the graphical output of the qualitative LDA with clearly separated seven clusters corresponding to each saccharide analyte measured in 20 repetitions with 100 % classification accuracy.…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescence intensity was measured for each well using four different channels (see experimental section). The obtained data were then subjected to Student's T‐test and analysed using Linear Discriminant Analysis (LDA) [38,40–42] . Here, the LDA was used to discriminate among different species and classify all repetitions into single cluster for each saccharide.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, there are only few reports on such simple and practical methods. [34][35][36][37][38] An effort toward this end are described below.…”

Section: Introductionmentioning

confidence: 99%

“…Particularly the methods that do not require specialized equipment or highly trained personnel could be of benefit to a large population. Interestingly, there are only few reports on such simple and practical methods [34–38] . An effort toward this end are described below.…”

Section: Introductionmentioning

confidence: 99%

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Binaphthalene Boronic Acid Sensor for Saccharides and d‐Fructose Determination in Beverages

et al. 2022

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The presence of electron rich compounds such as amines added to the fluorescent methoxybinaphthalene boronic acid results in a dramatic increase in affinity of diols to the aryl boronic acid as well as in the augmented fluorescence response. This is likely the result of the change in boron geometry upon coordination with electron donor which facilitate the diols binding. Here, we demonstrate the role of amino alcohol additive in binding of saccharides by boronic acid‐based fluorescent sensor. We show that this strategy allows a poorly responsive sensor to become a highly sensitive probe for the detection of sugars, which could be used for classification of saccharides as well as for quantitative analysis in DMSO‐water solutions. The simple binaphthalene boronic acid sensor was particularly sensitive to d‐fructose (Ka=2.08×106), which allowed for identification of commercial sweetened beverages based on their d‐fructose content. The same method was successfully used for the quantitative analysis of d‐fructose in soft beverages.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Interestingly, there are only few reports on such simple and practical methods. [34][35][36][37][38] An effort toward this end are described below.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Binaphthalene Boronic Acid Sensor for Saccharides and d‐Fructose Determination in Beverages

et al. 2022

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“… However, like most diboronates from the older literature, the classical diboronate 10 is not well soluble in water. For this reason, prior studies on diboronates for carbohydrate measurements often employ partial organic solvents (typically 50% methanol). − Diboronate 8 is well soluble in water and thus directly applicable to measurements in aqueous solutions. However, when tried with buffers including albumin in native concentration (0.5 mM), we found the fluorescent signal to be largely quenched.…”

Section: Introductionmentioning

confidence: 99%

Diboronate Fluorophore for the Measurement of l-Glucose and Other Carbohydrates and Its Interaction with Albumin

et al. 2022

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l -Glucose has recently been investigated as an artificial sweetener, but no facile method is established for the measurement of l -glucose. The commercial probe Eversense employs a fluorescent diboronate in a small device for the optical monitoring of d -glucose in people with diabetes. Being achiral, the Eversense probe should be able to detect l -glucose as well as native d -glucose, but the probe is designed for fixation under the skin, and our attempts to use the probe at laboratory conditions failed, as the probe was resetting when moved between compartments. We thus designed a water-soluble anthracene diboronate 8 similar to the fluorophore used in Eversense and found 8 to respond well to l -glucose and other carbohydrates and artificial sweeteners, thus enabling measurements of l -glucose with the limit of quantification of 12 μM. Notably, the fluorescent signal of diboronate 8 was largely quenched in buffers with the physiological concentration of albumin (0.5 mM), so the given analytical method would need more optimization to be useful for measuring l -glucose and other carbohydrates in blood samples. We suspect that other diboronate fluorophores from the literature may be similarly quenched if applied in the presence of albumin.

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Printed 384‐Well Microtiter Plate on Paper for Fluorescent Chemosensor Arrays in Food Analysis

Lyu

Sasaki

Ohshiro

et al. 2022

Chemistry An Asian Journal

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We propose a printed 384-well microtiter paperbased fluorescent chemosensor array device (384-well microtiter PCAD) to simultaneously categorize and discriminate saccharides and sulfur-containing amino acids for food analysis. The 384-well microtiter PCAD requiring 1 μL/4 mm 2 of each well can allow high-throughput sensing. The device embedded with self-assembled fluorescence chemosensors displayed a fingerprint-like response pattern for targets, the image of which was rapidly captured by a portable digital camera. Indeed, the paper-based chemosensor array system combined with imaging analysis and pattern recognition techniques not only successfully categorized saccharides and sulfur-containing amino acids but also classified mono-and disaccharide groups. Furthermore, the quantitative detectability of the printed device was revealed by a spike and recovery test for fructose and glutathione in a diluted freshly made tomato juice. We believe that the 384-well microtiter PCAD using the imaging analysis system will be a powerful sensor for multi-analytes at several categorized groups in real samples.

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Fluorescent Sensor Array for Quantitative Determination of Saccharides

Cited by 35 publications

References 62 publications

Binaphthalene Boronic Acid Sensor for Saccharides and d‐Fructose Determination in Beverages

Binaphthalene Boronic Acid Sensor for Saccharides and d‐Fructose Determination in Beverages

Diboronate Fluorophore for the Measurement of l-Glucose and Other Carbohydrates and Its Interaction with Albumin

Printed 384‐Well Microtiter Plate on Paper for Fluorescent Chemosensor Arrays in Food Analysis

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