Fluorescent Sensors for Biological Metal Ions

Kaur, Amandeep; Lim, Zelong; Yang, Kylie; New, Elizabeth J.

doi:10.1016/b978-0-12-409547-2.12612-5

Cited by 7 publications

(8 citation statements)

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“…The binding-based approach to sensing metals and other analytes has been used for many decades to elucidate biological processes. − A common strategy involves design of the sensor such that it undergoes self-quenching via excited-state electron transfer processes between the ligand/linker and the fluorophore, where subsequent metal binding will lower the energy of the ligand/linker electron donating orbital, thus preventing the electron transfer from occurring during fluorescence excitation and resulting in a fluorescent turn-on response . For the ligand scaffold design, selectivity is typically conferred using foundational principles of inorganic chemistry such as hard–soft acid–base (HSAB) theory, preferred denticity and coordination geometries, and location on the Irving–Williams series, which predicts the relative stabilities of 3d transition metal complexes . Activity-based sensors (also known as reactivity-based sensors), on the other hand, exploit the unique reactivity of a metal in order to carry out chemistry that results in an optical change. , Such reactivity typically involves using a metal’s ability to promote redox or Lewis acid catalysis to release a caged fluorophore, resulting in a turn-on or ratiometric change in fluorescence.…”

Section: Fluorescent Metal Sensors: Foundational Design Principlesmentioning

confidence: 99%

“…This pool is commonly termed the bioavailable pool, or labile pool. Metal pools that are tightly bound to proteins have a lower propensity to induce unwanted interactions since their reactivity is modulated by the three-dimensional protein active site …”

Section: Introductionmentioning

confidence: 99%

“…Metal pools that are tightly bound to proteins have a lower propensity to induce unwanted interactions since their reactivity is modulated by the threedimensional protein active site. 5 Because of the dual essential/toxic nature of redox-active metals, cells have evolved sophisticated machinery to sense and control metal pools, particularly the bioavailable metal pool. This network includes tightly regulated metal import proteins, metallochaperones, metal storage proteins, and metal export proteins, which work together in concert to ensure that metals are acquired and delivered to their sites of activity without causing unwanted damage to the cell.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Grover,

Koblova,

Pezacki

et al. 2024

Chem. Rev.

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Although transition metals constitute less than 0.1% of the total mass within a human body, they have a substantial impact on fundamental biological processes across all kingdoms of life. Indeed, these nutrients play crucial roles in the physiological functions of enzymes, with the redox properties of many of these metals being essential to their activity. At the same time, imbalances in transition metal pools can be detrimental to health. Modern analytical techniques are helping to illuminate the workings of metal homeostasis at a molecular and atomic level, their spatial localization in real time, and the implications of metal dysregulation in disease pathogenesis. Fluorescence microscopy has proven to be one of the most promising non-invasive methods for studying metal pools in biological samples. The accuracy and sensitivity of bioimaging experiments are predominantly determined by the fluorescent metal-responsive sensor, highlighting the importance of rational probe design for such measurements. This review covers activity-and binding-based fluorescent metal sensors that have been applied to cellular studies. We focus on the essential redox-active metals: iron, copper, manganese, cobalt, chromium, and nickel. We aim to encourage further targeted efforts in developing innovative approaches to understanding the biological chemistry of redox-active metals. CONTENTS5908 8.2. Activity-Based Fluorescent Sensors for Ni(II) 5911 9. Outlook 5911 Author Information 5912 Corresponding Authors 5912

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Section: Fluorescent Metal Sensors: Foundational Design Principlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Grover,

Koblova,

Pezacki

et al. 2024

Chem. Rev.

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show abstract

“…Recent efforts to prepare fluorescent sensors for Ag(I) have included systems based on gold nanoparticles [26], carbon dots [27,28] and polyoxy-derivatized perylenediimide [29]. More traditional metal-sensing systems, involving a silver-selective receptor coupled to a fluorophore [30], have used a range of donor atoms for the soft Ag(I), from hard nitrogen and oxygen donors [31] to the very soft tellurium [32]. In sensing Ag(I), there is a particular challenge in achieving selectivity over other metal ions, with the main interferents being iron and copper [33,34], and mercury [35,36].…”

Section: Introductionmentioning

confidence: 99%

A reversible fluorescent probe for monitoring Ag(I) ions

Lim

Smith

Kolanowski

et al. 2018

J. R. Soc. Interface.

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Silver-containing nanomaterials are of interest for their antibiotic properties, for a wide range of applications from medicine to consumer products. However, much remains to be learnt about the degradation of such materials and their effects on human health. While most analyses involve measurement of total silver levels, it is important also to be able to measure concentrations of active free Ag(I) ions. We report here the preparation of a coumarin-based probe, thiocoumarin silver sensor 1 (), that responds reversibly to the addition of silver ions through the appearance of a new fluorescence emission peak at 565 nm. Importantly, this peak is not observed in the presence of Hg(II), a common interferent in Ag(I) sensing. To establish the utility of this sensor, we prepared silver-doped phosphate glasses with demonstrated bactericidal properties, and observed the Ag(I) release from these glasses in solutions of different ionic strength. is therefore a useful tool for the study of the environmental and medical effects of silver-containing materials.

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“…Excimers are transient species formed when two fluorophore molecules of the same type interact through π orbitals, with one in the excited state and the other in the ground state. Their formation is influenced by excitation and local concentration, resulting in red-shifted fluorescence characteristics compared to the monomer . Despite our ability to control the assembly modes of organic dyes on DNA through factors like DNA sequence and dye concentration, there is still a need for systematic research on the diverse assembly modes of dyes on DNA and a deeper understanding of the factors influencing molecular assembly behavior.…”

mentioning

confidence: 99%

DNA Framework-Engineered Assembly of Cyanine Dyes for Structural Identification of Nucleic Acids

Li,

Chen,

Man

et al. 2024

JACS Au

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DNA nanostructures serve as precise templates for organizing organic dyes, enabling the creation of programmable artificial photonic systems with efficient light-harvesting and energy transfer capabilities. However, regulating the organization of organic dyes on DNA frameworks remains a great challenge. In this study, we investigated the factors influencing the self-assembly behavior of cyanine dye K21 on DNA frameworks. We observed that K21 exhibited diverse assembly modes, including monomers, H-aggregates, J-aggregates, and excimers, when combined with DNA frameworks. By manipulating conditions such as the ion concentration, dye concentration, and structure of DNA frameworks, we successfully achieved precise control over the assembly modes of K21. Leveraging K21's microenvironment-sensitive fluorescence properties on DNA nanostructures, we successfully discriminated between the chirality and topology structures of physiologically relevant G-quadruplexes. This study provides valuable insights into the factors influencing the dynamic assembly behavior of organic dyes on DNA framework nanostructures, offering new perspectives for constructing functional supramolecular aggregates and identifying DNA secondary structures.

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Fluorescent Sensors for Biological Metal Ions

Cited by 7 publications

References 106 publications

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals

A reversible fluorescent probe for monitoring Ag(I) ions

DNA Framework-Engineered Assembly of Cyanine Dyes for Structural Identification of Nucleic Acids

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