Fluorescent silver nanoclusters stabilized by DNA scaffolds

Yuan, Zhiqin; Chen, Ying-Chieh; Li, Hung‐Wen; Chang, Huan‐Tsung

doi:10.1039/c4cc02981j

Cited by 164 publications

(123 citation statements)

References 195 publications

(195 reference statements)

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“…58 Their results are similar to our results on silver clusters. They concluded that their lowest energy structures were the triangle for Au 3 , trapezoid (called diamond in this work) for Au 4 , X (bowtie) for Au 5 , W (trapezoid) for Au 5 + , and triangle for Au 6 . 58…”

Section: -3mentioning

confidence: 99%

“…Many studies have been performed to understand the effects from the DNA base pairs on the small AgNCs (∼Ag 2 -Ag 30 ) 18,[29][30][31][32] and some provide insights into creating AgNCs using different DNA platforms. 4,5,8,10 Their findings indicate that it is possible to manipulate the size and formation of these nanomaterials by employing strands of DNA with specific sequences. 3,18,29,30,32 Dickson, Petty, and coworkers have shown that very small (n < 10) AgNCs can be synthesized by exploiting the DNA sequence.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22] Therefore, DNA-AgNCs have been identified as potential candidates to be utilized in various applications such as bioimaging, biolabeling, catalytic reactions, and analyte and ligand sensing. [4][5][6][7]14,[23][24][25][26] The emission tunability can also be altered through experimental conditions, 19,20,27,28 but changing the DNA template itself is considered superior due to the higher functionality in the DNA-AgNCs through emission tuning. 13 Various research groups have conducted extensive experimental investigations on DNA-AgNCs.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Small sized metal nanoclusters (with diameters up to around 2 nm) are considered to be brighter and more photostable fluorophores [4][5][6][7][8][9][10][11] compared to existing organic dyes as well as smaller and less toxic compared to quantum dots. 12 In particular, silver nanoclusters (AgNCs) are of interest, and these systems involve stabilizing ligands to prevent them from oxidation and aggregation.…”

Section: Introductionmentioning

confidence: 99%

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Research Update: Density functional theory investigation of the interactions of silver nanoclusters with guanine

2017

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Bare and guanine-complexed silver clusters Ag n z (n = 2-6; z = 0-2) are examined using density functional theory to elucidate the geometries and binding motifs that are present experimentally. Whereas the neutral systems remain planar in this size range, a 2D-3D transition occurs at Ag 5 + for the cationic system and at Ag 4 2+ for the dicationic system. Neutral silver clusters can bind with nitrogen 3 or with the pi system of the base. However, positively charged clusters interact with nitrogen 7 and the neighboring carbonyl group. Thus, the cationic silver-DNA clusters present experimentally may preferentially interact at these sites. © 2017 Author (s)

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Section: -3mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Research Update: Density functional theory investigation of the interactions of silver nanoclusters with guanine

2017

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“…Attachment of ultra-bright BODIPY dyes and semiconductor nanocrystals called quantum dots (QDs) is especially promising for applications in fluorescent microscopy ( Figure 3D) [28,29]. In addition, fluorescent metal nanoclusters (for example, silver-or gold-based) have attractive optical qualities, which may increase the sensitivity of nucleic acid detection [30,31]. Remarkably, metal nanoclusters can detect natural nucleic acids, such as cancer related mRNAs, directly in living cells [31].…”

Section: Enzyme-free Hybridization Assays: Specificity In Focusmentioning

confidence: 99%

Novel Signal-Enhancing Approaches for Optical Detection of Nucleic Acids—Going beyond Target Amplification

Miotke

Barducci²,

Astakhova³

2015

Chemosensors

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Detection of low-abundance nucleic acids is a challenging task, which over the last two decades has been solved using enzymatic target amplification. Enzymatic synthesis enhances the signal so that diverse, scientifically and clinically relevant molecules can be identified and studied, including cancer DNA, viral nucleic acids, and regulatory RNAs. However, using enzymes increases the detection time and cost, not to mention the high risk of mistakes with amplification and data alignment. These limitations have stimulated a growing interest in enzyme-free methods within researchers and industry. In this review we discuss recent advances in signal-enhancing approaches aimed at nucleic acid diagnostics that do not require target amplification. Regardless of enzyme usage, signal enhancement is crucial for the reliable detection of nucleic acids at low concentrations. We pay special attention to novel nanomaterials, fluorescence microscopy, and technical advances in detectors for optical assessment. We summarize sensitivity parameters of the currently available assays and devices which makes this review relevant to the broad spectrum of researchers working in fields from biophysics, to engineering, to synthetic biology and bioorganic chemistry.

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Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Mok

Loh

et al. 2016

Adv Healthcare Materials

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thiolate-protected, [ 3 ] protein/peptide-templated, [ 4 ] and DNA-templated [ 5 ] ones. The last two types of metal NCs use biomolecules as templates to direct the synthesis (thus termed as biotemplated metal NCs), which afford more attractive features such as good aqueous solubility, superb biocompatibility, and versatile surface chemistries, [ 6 ] in addition to their ultrasmall size and superior photoluminescent optical properties. These desirable features render biotemplated metal NCs as promising candidates for various biomedical applications like imaging, [ 7 ] sensing, [ 7b,8 ] delivery [ 9 ] and therapeutics. [ 10 ] Theranostics is a revolutionary treatment strategy proposed by Funkhouser in 1998, which combines accurate diagnosis and targeted therapeutics. [ 11 ] From the list of individal biomedical applications mentioned earlier, a combination of sensing and imaging modalities would be fundamental for disease diagnosis. [ 12 ] In the same vein, image-guided targetedtherapy allows for real-time in vivo monitoring of the pharmacokinetics and pharmacodynamics of the therapeutic agent to enhance treatment effi ciency. [ 13 ] Through the ingenious combination of selective modalities, a highly potent and personalized theranostic agent could be developed. In particular, NC-based systems are poised to be the next versatile theranostic platform. This is because the intrinsic photoluminescence of the noble metal core can be harnessed for fl uorescence imaging, and tailor-made to exhibit unique optical properties for therapy, such as the generation of singlet oxygen [ 14 ] to substitute organicbased photosensitizers for possible photodynamic cancer treatments. In addition to the metal core, the biomolecular ligand shell surrounding the metal NC core can be easily functionalized with biorecognition moieties for sensing and targeted delivery. Due to the large surface to volume ratio of metal NCs, it can also serve as a reservoir sink to load multiple drugs/ genes and be modifi ed with sensing/imaging agents to imbue both therapeutic and diagnostic functions.The selective marriage of respective modalities to form theranostic biotemplated metal NCs will have a far-reaching impact on the future of medicine and healthcare. There are large amounts of work that use randomly picked natural biomolecules as templates to synthesize metal NCs for biomedical

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Fluorescent silver nanoclusters stabilized by DNA scaffolds

Cited by 164 publications

References 195 publications

Research Update: Density functional theory investigation of the interactions of silver nanoclusters with guanine

Research Update: Density functional theory investigation of the interactions of silver nanoclusters with guanine

Novel Signal-Enhancing Approaches for Optical Detection of Nucleic Acids—Going beyond Target Amplification

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

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