Fluorescent stereo microscopy for 3D surface profilometry and deformation mapping

Abstract

Abstract:Recently, mechanobiology has received increased attention. For investigation of biofilm and cellular tissue, measurements of the surface topography and deformation in real-time are a pre-requisite for understanding the growth mechanisms. In this paper, a novel threedimensional (3D) fluorescent microscopic method for surface profilometry and deformation measurements is developed. In this technique a pair of cameras are connected to a binocular fluorescent microscope to acquire micrographs from two different viewing angles of a sample surface doped or sprayed with fluorescent microparticles. Digital image correlation technique is used to search for matching points in the pairing fluorescence micrographs. After calibration of the system, the 3D surface topography is reconstructed from the pair of planar images. When the deformed surface topography is compared with undeformed topography using fluorescent microparticles for movement tracking of individual material points, the full field deformation of the surface is determined. The technique is demonstrated on topography measurement of a biofilm, and also on surface deformation measurement of the biofilm during growth. The use of 3D imaging of the fluorescent microparticles eliminates the formation of bright parts in an image caused by specular reflections. The technique is appropriate for non-contact, full-field and real-time 3D surface profilometry and deformation measurements of materials and structures at the microscale. Kormos, B. P. Griffith, H. Koyanagi, and J. F. Antaki, "High-resolution fluorescent particle-tracking flow visualization within an intraventricular axial flow left ventricular assist device," Artif. Organs 20(5), 534-540 (1996). 7. J. Sakakibara, K. Hishida, and M. Maeda, "Vortex structure and heat transfer in the stagnation region of an impinging plane jet (simultaneous measurements of velocity and temperature fields by digital particle image velocimetry and laser-induced fluorescence)," Int. J. Heat Mass Tran. 40(13), 3163-3176 (1997). 278-288 (2004). 38. M. A. Sutton, X. Ke, S. M. Lessner, M. Goldbach, M. Yost, F. Zhao, and H. W. Schreier, "Strain field measurements on mouse carotid arteries using microscopic three-dimensional digital image correlation," J.

A comparison of 2D and 3D digital image correlation for a membrane under inflation

“…Some of these conditions have motivated recent developments of techniques to measure 3D deformations using images acquired with a single camera [20–24]. Stereo-microscopy is also becoming increasingly popular for measurements on the microscale [25–27] however, it still suffers from a small depth of field at high magnification, a lower sensitivity in the out-of-plane measures compared to the in-plane measures [27] and a higher cost than traditional stereo-vision systems.…”

Internal Deformation Measurement and Force Chain Characterization of Mason Sand under Confined Compression using Incremental Digital Volume Correlation

“…DVC is an extension of the two-dimensional DIC technique [35][36][37]; it allows for non-contact, non-destructive, and fullfield measurement of the 3D internal deformation from the volumetric images acquired from 3D imaging techniques such as confocal microscopy, μ-CT, and magnetic resonance imaging (MRI).…”

A study of stereo microscope measurements based on interpolated feature matching

“…These useful tools have become increasingly popular in the field of biomedicine [1][2][3]. Several stereo matching methods have been applied in different cases.…”