Fluorescent ZnO for imaging and induction of DNA fragmentation and ROS-mediated apoptosis in cancer cells

Gupta, Jagriti; Bhargava, Parag; Bahadur, D.

doi:10.1039/c4tb01661k

Cited by 48 publications

(53 citation statements)

References 42 publications

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“…This work is the continuation of the previously published work [15] with the in-depth property studies of pristine zinc oxides and their photocatalytic behaviour towards other dye i.e. Rhodamine B (RhB) [13]. The microstructurals, chemical, defects and optical properties of these ZnO samples are explored using different characterisation techniques specifically XRD, SEM, TEM, HRTEM, FTIR, Raman, and ESR spectroscopy.…”

Section: Introductionmentioning

confidence: 91%

“…are increasingly gaining the limelight of today's research field as they have unique surface, optical, electrical, magnetic and others properties [1][2][3][4][5][6][7] that are limited to their nano regime but found to be almost insignificant in their bulk counterparts. Because of their exceptional properties in comparison to their difference [6] in bulk analogue, semiconductor photocatalyst materials are potentially used in various applications like solar cells [8], electrode materials [9,10], sensors [11,12], optical imagings [13], UV absorbers [14], photo-catalysis [15][16][17][18][19] etc. [2].…”

Section: Introductionmentioning

confidence: 99%

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Effect of induced defects on the properties of ZnO nanocrystals: surfactant role and spectroscopic analysis

2019

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Semiconductor photocatalysts are one of the major sought area of research due to their extensive use in the degradation of organic pollutants under UV-visible and solar light. Herein, we are reporting the synthesis of microscopic spindle shaped ZnO photocatalysts with different reducing agents such as sodium hydroxide, hydrazine hydride, sodium borohydride and lithium triethyl borohydride. These spindle shaped ZnO are synthesized by facile soft chemical approach by varying reducing agents with fixed molar ratio at 1:2 zinc precursor to reducing agents. These ZnOs were thoroughly characterised by various sophisticated characterization techniques such as XRD, FTIR, SEM, and HRTEM, Raman and ESR spectroscopies. Microscopic results show that these ZnO show similar fusiform morphologies with the variation in sizes and their distributions, and hence possess different defect related properties. The structural growth mechanism and defect related properties were explained on the basis of different reducing abilities of reducing agents (different reaction pH) and their nucleation with the aid of morphological analysis, and ESR analysis. Furthermore, the photocatalytic activity of these spindle shaped ZnO were evaluated against two cationic dyes (methylene blue dyes, Rhodamine B) under UV light. The photocatalytic results show that these spindle shaped ZnO are very effective for the photo-catalytic degradation of methylene blue and rhodamine B dye under the illumination of UV light.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effect of induced defects on the properties of ZnO nanocrystals: surfactant role and spectroscopic analysis

2019

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“…The cell viability loss induced by the composites is doserelated. 40 ZnO NPs was assumed to be responsible for the high antibacterial performance of ZnO NPs by initiating disorganization of the cell membrane and also ZnO damaged the membrane by releasing the zinc ion and producing the reactive oxygen species that is ROS dependent oxidative stress. However, over 50 µg/mL, NAD(P)H-dependent cellular oxidoreductase enzymes of more than 50% cells are not capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color, it may be due to the higher amount containing the ZnO particles, so, When the concentration of the composites increases to 250 µg/mL the viability decreased obviously.…”

Section: Cytotoxicity Assay Of the Go@cs/zno Compositesmentioning

confidence: 99%

A ZnO decorated chitosan–graphene oxide nanocomposite shows significantly enhanced antimicrobial activity with ROS generation

et al. 2015

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The rise in antimicrobial resistance requires the development of new antibacterial agents. Herein, we develop nanocomposite by growing zinc oxide nanoparticles on the surface of chitosan (CS) modified graphene oxide (GO), obtaining GO@CS/ZnO as a novel antibacterial material. The crystal structures, surface functional groups and morphology were analyzed by using X-ray diffraction (XRD) pattern, Fourier transform infrared spectroscopy (FTIR) scanning electron microscopy (SEM) images, respectively. The Minimum Inhibitory concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of GO@CS/ZnO show more antibacterial potency toward both Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive bacteria Staphylococcus aureus (S. aureus). In this study the GO@CS/ZnO expresses its more potency than reported graphene based nanocomposites. Investigation of intercellular reactive oxygen species (ROS) generation, NO generation and catalase activity in E. coli and S. aureus reveal that GO@CS/ZnO treatment also augments the intracellular bacterial killing by inducing reactive oxygen species production that causes oxidative damage. The minimal inhibition concentrations (MIC) of GO@CS/ZnO against E.coli and S. aureus are only 2.5 µg/mL and 5 µg/mL respectively. Compared with graphene based nanocomposite, which have been widely used as antibacterial agents, our GO@CS/ZnO shows better antibacterial effect. We envision that this study offers novel insights into antimicrobial actions and also demonstrates GO@CS/ZnO is a novel class of topical antibacterial agent in the areas of healthcare and environmental engineering.

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“…To investigate the possible mechanisms of the anti‐ proliferative effect of the in situ membrane calcification, the cell cycle and the apoptosis of the cells were investigated . As shown in Figure b‐d, the cells are arrested in S phaseafter in situ membrane calcification, the percentage of cells in S phase observed in the L1210@CaP (30.57%) is much higher than that in the native L1210 cells (21.6%) (Figure b), which is accompanied by a significant decrease of cells in G0/G1 phase (63.17% in L1210@CaP versus 70.63% in native L1210) and the similar G2‐phase cells in the two cell populations (6.37% in L1210@CaP versus 7.76% in native L1210). From these results, we could speculate that the cells were arrested in the S phase to inhibit cell proliferation after the in situ calcification.…”

Section: Figurementioning

confidence: 84%

A Targeting Membrane Injury Strategy via Calcification for the Inhibition of Leukemia Cells

Zhu

Wang

et al. 2019

ChemistrySelect

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Herein, a novel strategy was developed to inhibit leukemia cells via in situ calcification of biocompatible calcium phosphate targeting at cell membrane. Folic acid is selected as the adaptor of exogenous calcium/phosphate and leukemia cell membrane to induce in situ biomineralization and generate calcium phosphate on the membrane of leukemia cells. Our results demonstrate that the generated calcium phosphate coat significantly inhibits the proliferation of leukemia cells with the inhibition rate of 85%. Mechanistic studies indicate that the in situ calcification disrupts the integrity of cell membrane, increases the LDH leakage, damages the glycolysis ability, and induces the apoptosis. As such, the calcification strategy reported herein may be a potential leukemia therapy without the obvious side effect associated with standard chemotherapeutic drugs.

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Fluorescent ZnO for imaging and induction of DNA fragmentation and ROS-mediated apoptosis in cancer cells

Abstract: Systemic diagram shows the cell death mechanism through the generation of reactive oxygen species.

Cited by 48 publications

References 42 publications

Effect of induced defects on the properties of ZnO nanocrystals: surfactant role and spectroscopic analysis

Effect of induced defects on the properties of ZnO nanocrystals: surfactant role and spectroscopic analysis

A ZnO decorated chitosan–graphene oxide nanocomposite shows significantly enhanced antimicrobial activity with ROS generation

A Targeting Membrane Injury Strategy via Calcification for the Inhibition of Leukemia Cells

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