Fluoride induces oxidative stress and ATP depletion in the rat erythrocytes in vitro

Agalakova, Natalia I.; Гусев, Г. П.

doi:10.1016/j.etap.2012.05.006

Cited by 51 publications

(18 citation statements)

References 14 publications

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“…The participation of F − in free radical processes, including those involving ROS, has been confirmed in numerous reports [17–19]. However, previous results concerning the activities of the antioxidant enzymes (superoxide dismutase SOD, glutathione peroxidase GPx, catalase CAT), and glutathione S-transferase (GST), the concentration of malondialdehyde (MDA), and the total antioxidant potential (ABTS) do not present a uniform picture.…”

Section: Resultsmentioning

confidence: 94%

Effect of a Static Magnetic Fields and Fluoride Ions on the Antioxidant Defense System of Mice Fibroblasts

Kurzeja

Synowiec-Wojtarowicz

Stec

et al. 2013

IJMS

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The results of studies on the biological influence of magnetic fields are controversial and do not provide clear answers regarding their impact on cell functioning. Fluoride compounds are substances that influence free radical processes, which occur when the reactive forms of oxygen are present. It is not known whether static magnetic fields (SMF) cause any changes in fluoride assimilation or activity. Therefore, the aim of this work was to determine the potential relationship between magnetic field exposure to, and the antioxidant system of, fibroblasts cultured with fluoride ions. Three chambers with static magnetic fields of different intensities (0.4, 0.6, and 0.7 T) were used in this work. Fluoride ions were added at a concentration of 0.12 mM, which did not cause the precipitation of calcium or magnesium. The results of this study show that static magnetic fields reduce the oxidative stress caused by fluoride ions and normalize the activities of antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Static magnetic fields modify the energy state of fibroblasts, causing an increase in the ATP concentration and a decrease in the MDA concentration. These results suggest that exposure to fluoride and an SMF improves the tolerance of cells to the oxidative stress induced by fluoride ions.

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Section: Resultsmentioning

confidence: 94%

Effect of a Static Magnetic Fields and Fluoride Ions on the Antioxidant Defense System of Mice Fibroblasts

Kurzeja

Synowiec-Wojtarowicz

Stec

et al. 2013

IJMS

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“…Vice versa, an increased cellular arginine kinase activity was measured upon exposure of T. cruzi epimastigotes to hydrogen peroxide [19]. These findings suggested an important role of the phosphoarginine/arginine kinase system in oxidative stress response, a process that inherently consumes substantial amounts of ATP and eventually can lead to ATP-depletion [55], [56]. Also the different developmental stages of the exclusively extracellular parasite T. brucei are constantly exposed to oxidative stress, predominantly as a result of the innate immune response of its different hosts, but also as a consequence of its own unusual, partly aerobic fermentative energy metabolism [57], [58], [59], [60], [61].…”

Section: Discussionmentioning

confidence: 94%

The Phosphoarginine Energy-Buffering System of Trypanosoma brucei Involves Multiple Arginine Kinase Isoforms with Different Subcellular Locations

et al. 2013

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Phosphagen energy-buffering systems play an essential role in regulating the cellular energy homeostasis in periods of high-energy demand or energy supply fluctuations. Here we describe the phosphoarginine/arginine kinase system of the kinetoplastid parasite Trypanosoma brucei, consisting of three highly similar arginine kinase isoforms (TbAK1-3). Immunofluorescence microscopy using myc-tagged protein versions revealed that each isoform is located in a specific subcellular compartment: TbAK1 is exclusively found in the flagellum, TbAK2 in the glycosome, and TbAK3 in the cytosol of T. brucei. The flagellar location of TbAK1 is dependent on a 22 amino acid long N-terminal sequence, which is sufficient for targeting a GFP-fusion protein to the trypanosome flagellum. The glycosomal location of TbAK2 is in agreement with the presence of a conserved peroxisomal targeting signal, the C-terminal tripeptide ‘SNL’. TbAK3 lacks any apparent targeting sequences and is accordingly located in the cytosol of the parasite. Northern blot analysis indicated that each TbAK isoform is differentially expressed in bloodstream and procyclic forms of T. brucei, while the total cellular arginine kinase activity was 3-fold higher in bloodstream form trypanosomes. These results suggest a substantial change in the temporal and spatial energy requirements during parasite differentiation. Increased arginine kinase activity improved growth of procyclic form T. brucei during oxidative challenges with hydrogen peroxide. Elimination of the total cellular arginine kinase activity by RNA interference significantly decreased growth (>90%) of procyclic form T. brucei under standard culture conditions and was lethal for this life cycle stage in the presence of hydrogen peroxide. The putative physiological roles of the different TbAK isoforms in T. brucei are further discussed.

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“…However, only the highest fluoride dose significantly decreased GSH levels compared to control, denoting that fluoride can act as inhibitor of enzymatic antioxidants (CAT) or nonenzymatic antioxidants (GSH). Several studies report reduced enzymatic activity after fluoride exposure, followed by oxidative damage [ 48 – 50 ]. Other studies show that fluoride reduces CAT activity and GSH levels, but not the activity of SOD activity in human erythrocytes [ 49 ] and in the liver of mice [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies reported fluoride as an inducer of oxidative stress and modulator of intracellular redox homeostasis, lipid peroxidation, and protein carbonyl content [ 7 , 49 , 50 ]. These processes occur due to the ability to break hydrogen bonds in proteins (e.g., in the enzyme active center), as well as increasing the mitochondrial generation of free radicals resulting in oxidative stress, mitochondrial DNA degradation, and cell death [ 10 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Chronic Exposure to Sodium Fluoride Triggers Oxidative Biochemistry Misbalance in Mice: Effects on Peripheral Blood Circulation

Miranda

Gomes

Bittencourt

et al. 2018

Oxidative Medicine and Cellular Longevity

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The excessive fluoride (F) exposure is associated with damage to cellular processes of different tissue types, due to changes in enzymatic metabolism and breakdown of redox balance. However, few studies evaluate doses of F compatible with human consumption. Thus, this study evaluated the effects of chronic exposure to sodium fluoride (NaF) on peripheral blood of mice from the evaluation of biochemical parameters. The animals were divided into three groups (n = 10) and received three concentrations of NaF in the drinking water for 60 days: 0 mg/L F, 10 mg/L F, and 50 mg/L F. The blood was then collected for trolox equivalent antioxidant capacity (TEAC), thiobarbituric acid reactive substances (TBARS), concentrations of nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH). The results showed that doses of 10 mg/L F and 50 mg/L F were able to increase TBARS concentration and decrease NO levels and CAT activity in the blood, but there was no statistical difference for SOD levels. The 50 mg/L F group showed an increase in TEAC levels and a decrease in the GSH content when compared to the control group. In this way, oxidative changes in blood from chronic exposure to F, especially at the highest dose, indicate that F may be a toxic agent and, therefore, the long-term exposure to excessive doses should be avoided.

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Fluoride induces oxidative stress and ATP depletion in the rat erythrocytes in vitro

Cited by 51 publications

References 14 publications

Effect of a Static Magnetic Fields and Fluoride Ions on the Antioxidant Defense System of Mice Fibroblasts

Effect of a Static Magnetic Fields and Fluoride Ions on the Antioxidant Defense System of Mice Fibroblasts

The Phosphoarginine Energy-Buffering System of Trypanosoma brucei Involves Multiple Arginine Kinase Isoforms with Different Subcellular Locations

Chronic Exposure to Sodium Fluoride Triggers Oxidative Biochemistry Misbalance in Mice: Effects on Peripheral Blood Circulation

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