The results of studies on the biological influence of magnetic fields are controversial and do not provide clear answers regarding their impact on cell functioning. Fluoride compounds are substances that influence free radical processes, which occur when the reactive forms of oxygen are present. It is not known whether static magnetic fields (SMF) cause any changes in fluoride assimilation or activity. Therefore, the aim of this work was to determine the potential relationship between magnetic field exposure to, and the antioxidant system of, fibroblasts cultured with fluoride ions. Three chambers with static magnetic fields of different intensities (0.4, 0.6, and 0.7 T) were used in this work. Fluoride ions were added at a concentration of 0.12 mM, which did not cause the precipitation of calcium or magnesium. The results of this study show that static magnetic fields reduce the oxidative stress caused by fluoride ions and normalize the activities of antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Static magnetic fields modify the energy state of fibroblasts, causing an increase in the ATP concentration and a decrease in the MDA concentration. These results suggest that exposure to fluoride and an SMF improves the tolerance of cells to the oxidative stress induced by fluoride ions.

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Electron paramagnetic resonance (EPR) spectroscopy, UV-Vis spectrophotometry, and colorimetric analysis, were used to examine nonirradiated and UV-irradiated caffeic acid.In this work free radical formation during UV-irradiation of caffeic acid and the effect of UV-irradiation on its radical scavenging ability, were studied. The influence of time of UV-irradiation on these problems was evaluated. EPR spectra of free radicals in caffeic acid and 1,1-diphenyl-2-picrylo-hydrazyl (DPPH) were measured. DPPH was used as the model free radical reference to test antioxidative properties of caffeic acid. Amplitudes, linewidths, integral intensities, and g-factors were analyzed. Free radical concentrations were determined by comparing of the EPR spectra of caffeic acid and ultramarine-the paramagnetic reference. Free radicals were found (10 18 spin/g) in all the UV-irradiated samples independent on irradiation time. Free radical concentrations in caffeic acid increased with increasing of irradiation time. Analysis of spectral shape for different microwave powers pointed out that free radicals system in UV-irradiated caffeic acid was complex. Nonirradiated and UV-irradiated caffeic acid as antioxidants strongly interacted with free radicals and EPR signals of DPPH were highly quenched. UV-irradiation increased interactions of caffeic acid with free radicals, and this effect increased with increasing of irradiation time. Practical ApplicationThe performed studies indications that caffeic acid should not be storage exposed to UV. UV-irradiation formed free radicals in caffeic acid and changed its antioxidative properties as radical scavenging ability. Free radical concentrations in caffeic acid increased with increased UV irradiation time. UV-irradiation causes a change of color in caffeic acid. The change of color and formation of free radicals in caffeic acid during UV-irradiation can indicate the destruction of the chemical structure of tested samples.