Fluorimetric analysis of pesticides: Methods, recent developments and applications

Coly, Atanasse; Aaron, Jean‐Jacques

doi:10.1016/s0039-9140(97)00366-4

Cited by 78 publications

(31 citation statements)

References 130 publications

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“…The photodegradation mechanism certainly involves a modification of the aromatic moiety but is probably complex and therefore further investigation is necessary to characterize the structure of the photoproduct(s). However, from an analytical point of view, it is not really necessary to identify the structure of the fluorescent compound(s) formed (assuming reproducibility) [26]. Detection limits compared favorably with those obtained by LC-DAD and they are in the same order as those obtained by LC using MS detection and lower than the MRLs established in Spanish legislation.…”

Section: Introductionmentioning

confidence: 76%

Coupled‐column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables

García

Martínez

Galera

et al. 2004

J of Separation Science

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The coupled-column (LC-LC) system, consisting of a first column packed with internal surface reversed phase (ISRP) (50x4.6 mm ID) and a Chrompack C18 (100x4.6 mm ID) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (CH 2 Cl 2 ) extracts of vegetable samples without any clean-up step. This system was combined with a photochemically induced fluorescence (PIF) post-column derivatization in order to provide strongly fluorescent photoproducts from the non-fluorescent benzoylureas. Limits of detection ranged from 0.21 to 0.98 g L -1 of pesticide (equivalent to 0.14-0.65 g kg -1 in vegetable samples) and limits of determination ranged from 4.0 to 10.0 g L -1 (equivalent to 2.7-6.7 g kg -1 ). Linearity of the method was established between 2 and 1800 g L -1 , depending upon the compound.Validation of the total method was performed by randomly analyzing recoveries of four vegetable samples (aubergine, cucumber, green bean, and tomato) spiked at two levels of concentration (10.0 and 33.3 g kg -1 ). The combination of the LC-LC system with PIF detection provides a sensitive, selective, and rapid method for the determination of pesticides in vegetable samples at levels lower than the maximum residue levels (MRLs) established for these compounds by Spanish legislation.

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Section: Introductionmentioning

confidence: 76%

Coupled‐column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables

García

Martínez

Galera

et al. 2004

J of Separation Science

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“…For this reason, HPLC with UV [4 -6] and MS detection [7,8] has been used to determine these BUs in some matrices. It was recently demonstrated that UV irradiation yields photochemical reactions in several classes of pesticides, whose photochemistry has been reviewed by Crosby [9] and Marcheterre [10], and more recently by Aaron [6]. BU insecticides are a group of non-fluorescent pesticides, which may be converted into fluorescent species by photochemical reactions [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…BU insecticides are a group of non-fluorescent pesticides, which may be converted into fluorescent species by photochemical reactions [11,12]. With respect to their photochemistry, only diflubenzuron has been studied by Coly et al [6,13], among several aromatic insecticides. An interesting property of photochemical reactions is that they can be easily implemented as detection system in a continuous system.…”

Section: Introductionmentioning

confidence: 99%

Solid‐phase microextraction for the determination of benzoylureas in orange juice using liquid chromatography combined with post‐column photochemically induced fluorimetry derivatization and fluorescence detection

Vázquez¹,

Mughari

Galera

2008

J of Separation Science

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A solid-phase microextraction (SPME) method has been developed for the determination of six benzoylureas (diflubenzuron, triflumuron, hexaflumuron, teflubenzuron, lufenuron, and flufenoxuron) in natural orange juice based on the direct immersion mode of a 60 microm polydimethylsiloxane/divinylbenzene fiber. An orange juice was obtained from blended, homogenized, and diluted ecological natural orange juice samples. An aliquot of 3 mL of a spiked sample was extracted under optimum SPME conditions. The determination of benzoylureas was carried out using HPLC combined with post-column photochemically induced fluorimetry derivatization and fluorescence detection. The limits of quantification obtained in matrix were within the range of 0.02 to 0.04 mg/kg and these limits are lower than the maximum residue levels established in Spanish regulations for all pesticides in this study. Recoveries in juice samples ranged between 85 and 110% and relative standard deviations between 1.8 and 7.4%.

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“…Although there is a number of methods employed to assay pesticides using different detectors as fluorimetric, [11][12][13][14][15][16][17] photometric [18][19][20] and electrochemical, [21][22][23] some problems still arise associated with the complexity of the matrices, particularly in the case of HS solutions where the ionic strength is so high that the common methods frequently fail. To overcome this difficulty, large dilution factors are often used but with prejudice to the sensitivity of the methods.…”

Section: Introductionmentioning

confidence: 99%

Determination of triazines in hemodialysis saline solutions by adsorptive stripping voltammetry after extraction in acetonitrile

Nascimento¹,

Bohrer²,

Carvalho³

et al. 2003

J. Braz. Chem. Soc.

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A determinação de herbicidas 2-metiltio-4,6-dialquilamino-1,3,5-triazina (triazinas) em soluções salinas utilizadas em hemodiálise foi investigada por voltametria. Os herbicidas foram detectados com grande sensibilidade nas soluções salinas, após extração em fase de acetonitrila (ACN), utilizando o eletrodo de mercúrio de gota pendente. O efeito de salting out originado pelo meio salino existente nas soluções viabilizou as extrações. A relação de volumes entre as fases salina e de ACN foi investigada para alcançar a melhor sensibilidade na detecção das triazinas. A especiação entre as triazinas investigadas (ametrina, desmetrina, prometrina e terbutrina) não foi possível e recuperações entre 88 e 107% foram calculadas em amostras fortificadas com os analitos. Utilizando esta metodologia, limites de detecção de 0,03 µmol L -1 foram calculados para as triazinas nas amostras salinas.A method for the voltammetric determination of 2-methylthio-4,6-dialkylamino-1,3,5-triazine (triazines) herbicides in hemodialysis (HD) saline solutions was developed. The herbicides were detected in the saline solutions at the hanging mercury drop electrode (HMDE) with high sensitivities only after extraction of the analytes in acetonitrile (ACN). The salting out effect originated by the saline environment existing in the solutions enabled the extractions. The volume ratio between the saline and ACN phases was investigated in order to find the best sensitivity to detect the triazines. The speciation amongst them (ametryn, desmetryn, prometryn and terbutryn) was not possible. Recoveries between 88 and 107% were calculated in spiked samples, and detection limits of 0.03 µmol L -1 were calculated for the triazines in the saline samples using this methodology. Keywords: herbicides, triazines, hemodialysis, voltammetry IntroductionThe growing interest in the use of pesticides in agricultural production and their negative environmental impact has led to a number of studies for the development of different analytical methods. Besides the frequently used chromatographic measurements, voltammetry, mainly with differential pulse modes 1-9 should be taken into consideration as a fast, easy and inexpensive alternative. Additionally, the sensitivity of the electrochemical methods, their applicability over an unusually wide concentration range, is highly satisfactory for meeting requirements of toxicological, ecotoxicological and environmental regulation enforcement. 10 Hemodialysis (HD) solutions are prepared by mixing pure water and a high-salt (HS) content solution (about 7.0 mol L -1 ) to compose the final HD solution that has a salt concentration of about 0.2 mol L -1 . It is used to maintain the blood ionic equilibrium of the patients during the hemodialysis sessions. The presence of contaminants in the water or in HS solutions must be detected before a hemodialysis session as the HD solution remains very close to the blood of the patients separated only by the dialysis membranes. To aggravate the problem, the dialysis membranes can dialysate ...

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Fluorimetric analysis of pesticides: Methods, recent developments and applications

Cited by 78 publications

References 130 publications

Coupled‐column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables

Coupled‐column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables

Solid‐phase microextraction for the determination of benzoylureas in orange juice using liquid chromatography combined with post‐column photochemically induced fluorimetry derivatization and fluorescence detection

Determination of triazines in hemodialysis saline solutions by adsorptive stripping voltammetry after extraction in acetonitrile

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