Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella (Lepidoptera: Pyralidae)

Vašíček, Ondřej; Papežíková, Ivana; Hyršl, Pavel

doi:10.14411/eje.2011.061

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…15−17 In order to obtain uninterrupted electron transfer, different types of composite materials (enzymes, polymers, dyes, metals, and metal oxides) have been proposed for H 2 O 2 sensing. Among several analytical techniques (electrochemical, 18 spectrophotometry, 19 chemiluminescence, 20 and fluorimetry 21 ), due to the industrial importance of H 2 O 2 , fluorimetry has been found to be a simple, fast, and sensitive approach for the detection of H 2 O 2 . Chakraborty et al 22 reported the fluorescence quenching of C-dots (turn-off) sensor for H 2 O 2 in the presence of Fe 2+ .…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H₂O₂, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

2019

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Fluorescent nitrogen-doped graphene oxide dots (NGODs) have been demonstrated as an on–off nanosensor for the detection of Hg 2+ , Au 3+ , and H 2 O 2 . As compared to l -cystine, where the luminescence signal recovery results from the detachment of Hg 2+ from the NGODs, signal recovery through l -ascorbic acid (turn-off–on model) has been attributed to the reduction of Hg 2+ to Hg 0 . The sustainable recovery of the photoluminescence signal is demonstrated using common citrus fruits containing vitamin C ( l -AA), suggesting a promising practical usage of this sensing system. Additionally, the sensitivity of NGOD- and AA-originated signal recovery from the Hg(II)–NGODs mixture has been successfully tested in Hg 2+ ion-spiked tap water from three different places. Mimic devices were executed and verified on the basis of characteristic spectral changes, and the possible utility of this system in electronic security and memory element devices has also been demonstrated. Considering an easy synthesis process and excellent performance of NGODs, this investigation opens up new opportunities for preparing high-quality fluorescent NGODs to meet the requirements of many applications.

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Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H₂O₂, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

2019

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“… 4 So, far several analytical techniques such as such as spectrophotometry. 5 Fluorimetry, 6 electrochemical 7 and chemiluminescence 8 have been employed for the determination of H 2 O 2 . Among these, the electrochemical method offers a rapid and simple approach for the sensitive detection of H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

Enhanced electron transfer mediated detection of hydrogen peroxide using a silver nanoparticle–reduced graphene oxide–polyaniline fabricated electrochemical sensor

et al. 2018

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The current study aims at the development of an electrochemical sensor based on a silver nanoparticlereduced graphene oxide-polyaniline (AgNPs-rGO-PANI) nanocomposite for the sensitive and selective detection of hydrogen peroxide (H 2 O 2 ). The nanocomposite was fabricated by simple in situ synthesis of PANI at the surface of rGO sheet which was followed by stirring with AEC biosynthesized AgNPs to form a nanocomposite. The AgNPs, GO, rGO, PANI, rGO-PANI, and AgNPs-rGO-PANI nanocomposite and their interaction were studied by UV-vis, FTIR, XRD, SEM, EDX and XPS analysis. AgNPs-rGO-PANI nanocomposite was loaded (0.5 mg cm À2 ) on a glassy carbon electrode (GCE) where the active surface area was maintained at 0.2 cm 2 for investigation of the electrochemical properties. It was found thatAgNPs-rGO-PANI-GCE had high sensitivity towards the reduction of H 2 O 2 than AgNPs-rGO which occurred at À0.4 V vs. SCE due to the presence of PANI (AgNPs have direct electronic interaction with N atom of the PANI backbone) which enhanced the rate of transfer of electron during the electrochemical reduction of H 2 O 2 . The calibration plots of H 2 O 2 electrochemical detection was established in the range of 0.01 mM to 1000 mM (R 2 ¼ 0.99) with a detection limit of 50 nM, the response time of about 5 s at a signal-to-noise ratio (S/N ¼ 3). The sensitivity was calculated as 14.7 mA mM À1 cm À2 which indicated a significant potential as a non-enzymatic H 2 O 2 sensor.

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“…Hemocytes were collected by centrifugation for 5 min at 400× g , counted with Trypan Blue to differentiate between dead and living hemocytes and resuspended in IPS. Cells were used immediately in the ThermoFisher Scientific Hydrogen Peroxide Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) to determine the relative levels of H 2 O 2 in hemocytes [ 40 ]. The assay was conducted by creating a 50 µM Amplex Red, 1 U/mL of Horse Radish Peroxidase (HRP) solution.…”

Section: Methodsmentioning

confidence: 99%

Serial Passage of Cryptococcus neoformans in Galleria mellonella Results in Increased Capsule and Intracellular Replication in Hemocytes, but Not Increased Resistance to Hydrogen Peroxide

et al. 2020

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To gain insight into how pathogens adapt to new hosts, Cryptococcus neoformans (H99W) was serially passaged in Galleria mellonella. The phenotypic characteristics of the passaged strain (P15) and H99W were evaluated. P15 grew faster in hemolymph than H99W, in vitro and in vivo, suggesting that adaptation had occurred. However, P15 was more susceptible to hydrogen peroxide in vitro, killed fewer mouse macrophages, and had less fungal burden in human ex vivo macrophages than H99W. Analysis of gene expression changes during Galleria infection showed only a few different genes involved in the reactive oxygen species response. As P15 sheds more GXM than H99W, P15 may have adapted by downregulating hemocyte hydrogen peroxide production, possibly through increased capsular glucuronoxylomannan (GXM) shedding. Hemocytes infected with P15 produced less hydrogen peroxide, and hydrogen peroxide production in response to GXM-shedding mutants was correlated with shed GXM. Histopathological examination of infected larvae showed increased numbers and sizes of immune nodules for P15 compared to H99W, suggesting an enhanced, but functionally defective, response to P15. These results could explain why this infection model does not always correlate with murine models. Overall, C. neoformans’ serial passage in G. mellonella resulted in a better understanding of how this yeast evolves under selection.

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Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella (Lepidoptera: Pyralidae)

Cited by 13 publications

References 31 publications

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H₂O₂, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H₂O₂, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

Enhanced electron transfer mediated detection of hydrogen peroxide using a silver nanoparticle–reduced graphene oxide–polyaniline fabricated electrochemical sensor

Serial Passage of Cryptococcus neoformans in Galleria mellonella Results in Increased Capsule and Intracellular Replication in Hemocytes, but Not Increased Resistance to Hydrogen Peroxide

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Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella (Lepidoptera: Pyralidae)

Cited by 13 publications

References 31 publications

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H2O2, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H2O2, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

Enhanced electron transfer mediated detection of hydrogen peroxide using a silver nanoparticle–reduced graphene oxide–polyaniline fabricated electrochemical sensor

Serial Passage of Cryptococcus neoformans in Galleria mellonella Results in Increased Capsule and Intracellular Replication in Hemocytes, but Not Increased Resistance to Hydrogen Peroxide

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Product

Resources

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Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H₂O₂, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks

Nitrogen-Doped Graphene Oxide Dots-Based “Turn-OFF” H₂O₂, Au(III), and “Turn-OFF–ON” Hg(II) Sensors as Logic Gates and Molecular Keypad Locks