Enhanced electron transfer mediated detection of hydrogen peroxide using a silver nanoparticle–reduced graphene oxide–polyaniline fabricated electrochemical sensor

Abstract

The current study aims at the development of an electrochemical sensor based on a silver nanoparticlereduced graphene oxide-polyaniline (AgNPs-rGO-PANI) nanocomposite for the sensitive and selective detection of hydrogen peroxide (H 2 O 2 ). The nanocomposite was fabricated by simple in situ synthesis of PANI at the surface of rGO sheet which was followed by stirring with AEC biosynthesized AgNPs to form a nanocomposite. The AgNPs, GO, rGO, PANI, rGO-PANI, and AgNPs-rGO-PANI nanocomposite and their interaction were studied by UV-vis, FTIR, XRD, SEM, EDX and XPS analysis. AgNPs-rGO-PANI nanocomposite was loaded (0.5 mg cm À2 ) on a glassy carbon electrode (GCE) where the active surface area was maintained at 0.2 cm 2 for investigation of the electrochemical properties. It was found thatAgNPs-rGO-PANI-GCE had high sensitivity towards the reduction of H 2 O 2 than AgNPs-rGO which occurred at À0.4 V vs. SCE due to the presence of PANI (AgNPs have direct electronic interaction with N atom of the PANI backbone) which enhanced the rate of transfer of electron during the electrochemical reduction of H 2 O 2 . The calibration plots of H 2 O 2 electrochemical detection was established in the range of 0.01 mM to 1000 mM (R 2 ¼ 0.99) with a detection limit of 50 nM, the response time of about 5 s at a signal-to-noise ratio (S/N ¼ 3). The sensitivity was calculated as 14.7 mA mM À1 cm À2 which indicated a significant potential as a non-enzymatic H 2 O 2 sensor.

Magnetite Nanorods Stabilized by Polyaniline/Reduced Graphene Oxide as a Sensing Platform for Selective and Sensitive Non‐enzymatic Hydrogen Peroxide Detection

“…The O 1s core level spectrum with deconvoluted peaks is shown in Figure S3 in Supporting Information. The peaks at 529.8 and 531.3 eV represent C=O and C−OH/C−O−C, respectively . Figure c shows the deconvoluted N 1s spectrum and three peaks were seen at 399, 400, and 402 eV, which are related to the =N−, −NH− and N +.…”

“…The prepared material exhibited excellent sensitivity, wide linear range from 5 μM to 9.255 mM, and low LOD of 0.110 μM. Thus, accordingly, it is expected that the integration of PANI along with rGO not only prohibit the restacking of rGO sheets, but also promotes electron transfer .…”

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

“…The difficulties associated with the use of biological sensing molecules have motivated researchers to use inorganic materials with mimicking enzymatic properties for H 2 O 2 detection. Some of those materials are silver NPs (AgNPs) [ 167 , 168 , 169 , 170 ], AuNPs [ 171 , 172 , 173 , 174 ], platinum NPs (PtNPs) [ 175 , 176 ], ZnO and ZnMn 2 O 4 [ 177 , 178 ], Ag and Au on ZnO nanoflowers [ 179 ], MnO 2 and Mn 3 O 4 [ 180 , 181 ], Co 3 O 4 [ 182 , 183 ], Co 9 S 8 hollow spheres [ 184 ], Cu 3 P nanowires [ 185 ], and lanthanide coordination polymer NPs [ 186 ].…”

2D Materials in Development of Electrochemical Point-of-Care Cancer Screening Devices

“…Many researches have been conducted using graphene nanosheets and rGO modified with various catalytically-active nanostructures and/or enzymes to further improve the performance of electrochemical quantification of ROS/RNS in situ and in vitro . For example, graphene-decorated nanoparticles, such as Au [111], Pt [112], CuS [113], Fe 3 O 4 [114], Ag [115], Cu [116], etc., have been successfully employed for monitoring H 2 O 2 level in body fluids (human serum and urine) and released from different cancer cells (lung cancer A549, liver cancer HepG2, and glioblastoma cell U87), which provided very useful information regarding the progress of tumor development. Additionally, towards the fabrication of implantable devices, Duan’s group developed a flexible sensor using rGO paper that was decorated with Au@Pt core-shell nanoparticles to form a 2D closely-packed-assemblies for screening the NO secretion by human umbilical vein endothelial cells (HUVECs) after being stimulated by acetylcholine (Ach) [117].…”