Fluorimetric determination of vitamin k3 (menadione sodium bisulfite) in synthetic animal feed by high-performance liquid chromatography using a post-column zinc reducer

A novel, sensitive method for determining benzo[a]pyrene-diones using high-performance liquid chromatography with post-column zinc reduction

“…However, the acidic mobile phase resulted in problems that were not reported in previous publications using a post-column zinc reducer [15,16]. Specifically, an unknown substance coated the flow cells of the detectors and resulted, eventually, in the inability to use the detectors.…”

“…However, BaP-hydroquinones are unstable in the presence of air and readily autoxidize to their BaP-diones [14]. To develop a simple technique to reduce BaP-diones to their respective hydroquinones, we adapted a method that had been used to analyze Vitamin K compounds by HPLC, in which Vitamins K 1 and K 3 were reduced in-line to their fluorescent analogues using reduction by zinc metal following the chromatographic separation [15,16]. Here we report the development and validation of a simple HPLC method based on this post-column zinc reducer strategy.…”

Microplate analytical method for quinones by pulse photo-irradiation and chemiluminescence detection

“…Although the high sensitivity achieved by the fluorescence detection, quinones lack native fluorescence and have to be reduced to their hydroquinone form prior to fluorescence detection. Different procedures are used for quinone reduction, either chemically, [13][14][15] electrochemically 16 or photochemically 17,18 . Although the fluorescence detection may accomplish the required sensitivity, the methods lacked simplicity and rapidity because the reduction processes required many additional systems component such as reducing column, electric reactor or photoreactor.…”

Determination of Menadione Sodium Bisulfite in Pharmaceutical Preparations by Flow-Injection On-line Photochemical Spectrofluorometry

“…[1][2][3][4][5][6] Although MSB is a non-fluorescent compound, it can be converted into a strongly fluorescent compound based on reduction of its hydrolyzed compound, menadione. The reduction of menadione may be conducted either chemically 7,8 or electrochemically 9 as well as photochemically. 10 Torro et al 11 developed a flow injection (FI) spectrofluorometry for the determination of MSB based on chemical reduction of the menadione by a solid-phase reactor packed with metal zinc powder.…”