Fluoro-Gold: An alternative viability stain for multicolor flow cytometric analysis

Barber, Lesley; Prince, H. Miles; Rossi, Ralph; Bertoncello, Ivan

doi:10.1002/(sici)1097-0320(19990801)36:4<349::aid-cyto10>3.0.co;2-1

Cited by 14 publications

(3 citation statements)

References 6 publications

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“…FcγIII/II receptor block was performed with anti‐mouse CD16/32 followed by staining with directly conjugated rat anti‐mouse CD45, CD31, Ter119, Sca‐1, and appropriate isotype controls (BD Biosciences). The fluorochromes, 7‐amino‐actinomycin D (2 μg/ml; Sigma) or Fluoro‐gold (FG) [16] (1 μg/ml; Molecular Probes Inc., Eugene, OR, http://probes.invitrogen.com) were used as viability dyes for flow cytometric detection and exclusion of nonviable cells.…”

Section: Methodsmentioning

confidence: 99%

Endogenous Fibroblastic Progenitor Cells in the Adult Mouse Lung Are Highly Enriched in the Sca-1 Positive Cell Fraction

et al. 2009

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Originally identified as a marker specifying murine hematopoietic stem cells, the Sca-1 antigen has since been shown to be differentially expressed by candidate stem cells in tissues including vascular endothelium, skeletal muscle, mammary gland, and prostate of adult mice. In the adult murine lung, Sca-1 has previously been identified as a selectable marker for the isolation of candidate nonhematopoietic (CD45 Sca-1 1 CD34 1 cell phenotype does define endogenous clonogenic progenitor cells in the adult murine lung, our data indicate that these progenitors are predominantly representative of mesenchymal cell lineages, and highlights the pressing need for the identification of alternative markers and robust functional assays for the identification and characterization of epithelial and fibroblastic stem and progenitor cell populations in the adult lung. STEM CELLS 2009;27:623-633 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Methodsmentioning

confidence: 99%

Endogenous Fibroblastic Progenitor Cells in the Adult Mouse Lung Are Highly Enriched in the Sca-1 Positive Cell Fraction

et al. 2009

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“…The use of nuclear dyes may be helpful for analysis where more exact determination of neoplastic cells is required, such as MRD analysis; and for establishing/confirming the presence of nucleated erythroid cells. Several reagents are available; examples of reports on their relative merits in the context of multi-colour panels include Barber et al (1999), Perfetto et al (2006b) and Mahnke and Roederer (2007).…”

Section: Specimen Quality: Viability and Nuclear Dyesmentioning

confidence: 99%

Guidelines on the use of multicolour flow cytometry in the diagnosis of haematological neoplasms

et al. 2014

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“…Alternatively, hydroxystilbamidine (Invitrogen/Life Technologies), also marketed as or Fluoro-Gold (Flurochrome, LLC; http://www.fluorochrome.com/), is a UV-or near UV-excited DNA-binding dye excluded from viable cells. Its brightness is similar to that of 7-AAD (Barber et al, 1999). SYBR-14, a DNA stain from Invitrogen/Life Technologies, penetrates viable cells (Garner et al, 1994).…”

Section: 219mentioning

confidence: 99%

Assessment of Cell Viability

Johnson

Nguyen

Coder³

2013

CP Cytometry

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Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes or the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre‐fixation viability of fixed cells either before or after fixation with amine‐reactive dyes suitable for a range of excitation wavelengths. Membrane‐impermeable dead cell and live cell dyes as well as dye‐exclusion procedures for microscopy are also included. Curr. Protoc. Cytom. 64:9.2.1‐9.2.26. © 2013 by John Wiley & Sons, Inc.

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Fluoro-Gold: An alternative viability stain for multicolor flow cytometric analysis

Cited by 14 publications

References 6 publications

Endogenous Fibroblastic Progenitor Cells in the Adult Mouse Lung Are Highly Enriched in the Sca-1 Positive Cell Fraction

Endogenous Fibroblastic Progenitor Cells in the Adult Mouse Lung Are Highly Enriched in the Sca-1 Positive Cell Fraction

Guidelines on the use of multicolour flow cytometry in the diagnosis of haematological neoplasms

Assessment of Cell Viability

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