Fluoroacetate

Гончаров, Н. В.; Savelieva, Elena; Зінченко, В. П.; Кузнецов, С. В.; Mindukshev, Igor; Vinokurov, Maxim; Avdonin, P. V.; Voitenko, Natalia; Ukolov, A. I.; Orlova, T. S.; Jenkins, Richard O.; Kuznetsov, A. Yu.

doi:10.1016/b978-0-12-800159-2.00016-6

Cited by 2 publications

(2 citation statements)

References 164 publications

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“…Fluoroacetamide (FAM) is a fluorine compound with tremendous toxicity, formerly used as a rodenticide and insecticide, which inhibits the citrate metabolism by blocking its transport into mitochondria and its breakdown by aconitase in vivo [1,2]. The mean lethal dose of FAM varies from 0.05 mg/kg for dogs to 150 mg/kg for possums, while 50 mg/kg is considered to be a lethal dose for humans [3][4][5]. The serum FAM in 65 cases of acute toxicosis patients had been monitored continuously; the peak value of the serum FAM appeared on the third hour and varied from 0.81 to 3.65 mg/L [6,7].…”

Section: Introductionmentioning

confidence: 99%

Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical

et al. 2020

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Fluoroacetamide (FAM) is a small (77 Da) and highly toxic chemical, formerly used as a rodenticide and potentially as a poison by terrorists. Poisoning with FAM has occurred in humans, but few reliably rapid detection methods and antidotes have been reported. Therefore, producing a specific antibody to FAM is not only critical for the development of a fast diagnostic but also a potential treatment. However, achieving this goal is a great challenge, mainly due to the very low molecular weight of FAM. Here, we design two groups of FAM haptens for the first time, maximally exposing the fluorine or amino groups, with the aid of linear aliphatic or phenyl-contained spacer arms. Interestingly, whereas the hapten with fluorine at the far end of the hapten did not induce an antibody response to FAM, the hapten with an amino group at the far end and phenyl-contained spacer arm triggered a significantly specific antibody response. Finally, a monoclonal antibody (mAb) named 5D11 was successfully obtained with an IC50 value of 97 μg mL−1 and negligible cross-reactivities to the other nine functional and structural analogs.

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Section: Introductionmentioning

confidence: 99%

Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical

et al. 2020

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“…The patent named methylene blue (MB) and monosodium glutamate (MSG) as a combined treatment to decrease the lethality of 1080 in rats. Data were presented in the patent, and preliminary data have been included in textbook chapters; however, no comprehensive peer‐reviewed article has been published 14,42 …”

Section: Introductionmentioning

confidence: 99%

Methylene blue and monosodium glutamate improve neurologic signs after fluoroacetate poisoning

Cox

Hartog

Pueblo

et al. 2020

Annals of the New York Academy of Sciences

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Fluoroacetate (FA) is a tasteless, odorless, water-soluble metabolic poison with severe toxicological effects. Characterized in the mid-1900s, it has been used as a rodenticide but is comparably lethal to all mammals. Many countries have restricted its use, and modern-day accidental human exposures are rare, but recently, concerns have been raised about its application as a chemical weapon with no known antidote. A combined treatment of methylene blue (MB), an antioxidant, and monosodium glutamate (MSG), a precursor of the citric acid cycle substrate alphaketoglutarate, has been recommended as an effective countermeasure; however, no peer-reviewed articles documenting the efficacy of this therapy have been published. Using a rodent model, we assessed the effects of MB and MSG on the neurologic, cardiac, and pulmonary systems. Transcriptomic analysis was used to elucidate inflammatory pathway activation and guide bioassays, which revealed the advantages and disadvantages of these candidate countermeasures. Results show that MB and MSG can reduce neurologic signs observed in rats exposed to sodium FA and improve some effects of intoxication. However, while this strategy resolved some signs of intoxication, ultimately it was unable to significantly reduce lethality.

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Fluoroacetate

Cited by 2 publications

References 164 publications

Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical

Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical

Methylene blue and monosodium glutamate improve neurologic signs after fluoroacetate poisoning

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