Fluorogenic Probes for Multicolor Imaging in Living Cells

Abstract

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.

Quantitative single-molecule study of TGF-β/Smad signaling

“…It is relatively difficult to study intracellular molecules due to the signal interference from the more complex intracellular environment. Moreover, it is still a major challenge to develop new fluorescent probes with improved brightness and photostability to meet the requirement for long-time intracellular tracking, and with fluorogenic property and proper labeling strategies to ensure specific intracellular labeling [17,19,[83][84][85][86].…”

Hydroxylated Fluorescent Dyes for Live‐Cell Labeling: Synthesis, Spectra and Super‐Resolution STED

“…On the contrary, several rhodamine‐type dyes specifically stain intact cells when applied as conjugates with small molecule recognition units . The most widely used recognition units are BG‐NH 2 , BC‐NH 2 and HaloTag amine (covalent ligands of the SNAP‐tag, CLIP‐tag, and HaloTag proteins).…”

A Highly Photostable Near‐Infrared Labeling Agent Based on a Phospha‐rhodamine for Long‐Term and Deep Imaging

“…These compounds show absorption (l abs )and emission (l em )m axima in the NIR region with large molar absorption coefficients (e % 1 10 5 Lmol À1 cm À1 )a nd sufficient fluorescence quantum yields (F F % 0.13). [12,13] To assess the potential utility of 1a-c as labeling reagents, we initially evaluated the chemical stability of these dyes against nucleophiles.The absorption changes of their aqueous solutions were monitored at pH 4.2, 7.4, and 10.3. Compound 1c,w hich bears a2 ,6-dimethoxyphenyl group,e xhibited l abs and l em values of 712 nm and 740 nm, respectively (Supporting Information, Figure S5), which are slightly longer wavelengths than those of 1a and 1b,and hence indicative of an electronic perturbation by the aryl group at the 9-position.…”