Fluorometric Assay of Alcohols Using Alcohol Oxidase

Guilbault, George G.; Sadar, S. H.

doi:10.1080/00032716908051270

Cited by 10 publications

(1 citation statement)

References 2 publications

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“…A linear relationship was observed for the initial response rate against ethanol concentration below 65 mg/L. The Michaelis constant for alcohol oxidase vs ethanol was determined by different investigators to be 46 mg/L (Janssen et al, 1968) and 84 mg/L (Guilbault et al, 1969). The upper detection limit of this alcohol sensor falls reasonably within this region.…”

Section: Resultsmentioning

confidence: 58%

An Amperometric Alcohol Sensor Based on Chemically Permeabilized Methylotrophic Microorganisms

Chen

Naglak

Wang

1992

Biotechnology Progress

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Chemical permeabilization of the yeast Pichia pastoris NRRL Y‐11430 with 1.0% glycine and 0.1 % Triton X‐100 does not release intracellular alcohol oxidase but alters the cell envelope structure, allowing alcohol oxidase activity to be detected inside the cells (Naglak, 1990). An alcohol sensor based on the use of chemically permeabilized yeast cells was constructed. The sensor response time was within 2 min. The assay time was less than 30s by the initial rate method. The calibration curve for ethanol analysis was linear in the range 5–65 mg/L. The sensor response was almost unaffected by pH changes within the range pH 5–9. This alcohol sensor produced the highest sensitivity for methanol; the sensitivity for ethanol was about half that for methanol. The stability and other characteristics of this new alcohol sensor are also discussed in this work.

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Section: Resultsmentioning

confidence: 58%