Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation

Lai, Qi; Liu, Qing; Zhao, Kai; Duan, Xinhe; Wang, Guannan

doi:10.1007/s00604-019-3438-1

Cited by 20 publications

(9 citation statements)

References 42 publications

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“…Amino acids, as the essential building blocks for organisms, are significant for biological systems . Cysteine is one of the most abundant small molecule biological thiols, which plays a unique role in peptide and protein synthesis, detoxification, and metabolism . Hence, it is meaningful to construct a fluorescent sensor to detect cysteine.…”

Section: Resultsmentioning

confidence: 99%

“…42 Cysteine is one of the most abundant small molecule biological thiols, which plays a unique role in peptide and protein synthesis, detoxification, and metabolism. 43 Hence, it is meaningful to construct a fluorescent sensor to detect cysteine. In this article, the superior luminescent aggregate, 2.0 mM 14-2-14•2Ce@0.55 mM GSH-Au NCs, could serve as a fluorometric probe to detect cysteine selectively and sensitively from 20 natural amino acids.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Amphiphilic Au Nanoclusters Modulated by Magnetic Gemini Surfactants as a Cysteine Chemosensor and an MRI Contrast Agent

Zhao

et al. 2021

Langmuir

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Cationic magnetic Gemini surfactants (mag-Gsurfs), [C 14 H 29 (CH 3 ) 2 N(CH 2 ) 2 N(CH 3 ) 2 C 14 H 29 ] 2+ •2[XCl 3 Br] − (14-2-14•2X, X = Ce, Gd, or Ho), efficiently induce the aggregation of glutathione-protected Au nanoclusters (NCs) (GSH-Au NCs). These magnetic luminescent aggregates not only possess aggregation-induced emission (AIE) behavior but also display aggregation-induced magnetic enhancement. In particular, 14-2-14•2Ce and 14-2-14•2Gd have a better effect on boosting the luminescence intensity, quantum yield (QY), and luminescence lifetime (τ). The luminescent aggregates of GSH-Au NCs triggered by 14-2-14•2Gd or 14-2-14•2Ho exhibit more favorable paramagnetic behavior. Other Au NCs containing a Au(I)−thiolate complex shell also exhibit the obvious AIE phenomenon after introducing 14-2-14•2Gd, demonstrating the luminescence enhancement effect of mag-G-surfs. The luminescent aggregate 14-2-14• 2Ce@GSH-Au NCs can serve as a "light up" fluorometric probe to detect cysteine selectively with the detection limit (DL) of 36 μM, and the magnetic luminescent aggregate 14-2-14•2Gd@GSH-Au NCs has the potential to be a novel contrast agent in T 1weighted magnetic resonance (MR) imaging due to its satisfactory contrasting ability.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Amphiphilic Au Nanoclusters Modulated by Magnetic Gemini Surfactants as a Cysteine Chemosensor and an MRI Contrast Agent

Zhao

et al. 2021

Langmuir

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“…Beneficial luminescent nanoclusters, such as Au, [22][23][24] Cu, 25 and Ag, 26 have been extensively focused on, of which AuNCs have been the frontrunner due to their high stabilities. 27 AIE has played a major role in the luminescence properties of nanoclusters, where the aggregation degree acts as a focal point for emission wavelength and the intensity. The AIE in NCs occurs through self-assembled routes (non-electrostatic and electrostatic), which usually results in considerable blueshifts in emission accompanied by the transformation from a disordered to an ordered morphology.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of cysteine and chiral discrimination of D-/L-cysteine via the aggregation-induced emission enhancement of gold nanoclusters by Ag+

Ruan

Zhang

et al. 2022

ANAL. SCI.

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Cysteine (Cys) plays vital roles in various physiological and pathological functions.Either deficiency or excess of Cys could lead to severe ailments in human. Identification and determination of Cys are the key issues for the early diagnosis of relevant diseases.This contribution presented a promising potential of fluorescent gold nanoclusters (AuNCs) for Cys determination and D-/L-Cys enantiomer discrimination. Cys determination and discrimination involved three steps. First, as a reducing and capping ligand, glutathione was applied to fabricate weak fluorescent AuNCs. Second, Ag + was introduced to lead the aggregation-induced emission (AIE) to form well-dispersed aggregates. The fluorescence intensity of AuNCs was monitored at excitation/ emission wavelengths of 396/620 nm. Third, Cys was found to quickly bind with Ag + to form a grid network to light up the system via aggregation-induced emission enhancement (AIEE). A novel sensor for sensitive and visually selective detection of Cys was established on the basis of AIEE mechanism. Rapid quantitative determination of Cys was achieved in two minutes via AIEE within the range of 0.5-100 μmol L −1 and a detection limit of 0.365 μmol L −1 . Moreover, due to the specific interactions of D-/L-Cys with mandelic acid and tartaric acid, visual discrimination of D-/L-Cys enantiomers with naked eyes was realized by replacing the organic acid buffer.

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“…AuNCs are typically colloidal nanoparticles, that are protected by proteins, peptides, or small organic molecules as capping agents. Among these nanoparticles, glutathione‐stabilized AuNCs (GSH‐AuNCs) show high‐fluorescence quantum yield (QY) due to aggregation‐induced emission (AIE) effects . This intrinsic feature enables the GSH‐AuNCs to be a highly sensitive fluorescence probe.…”

Section: Introductionmentioning

confidence: 99%

“…However, the AIE effect also has a negative influence on analyte detection. For instance, the polarity and impurities of a solution can facilitate the AIE effect of GSH‐AuNCs, resulting in instability of the fluorescence properties (intensity and maximum) . Additionally, analogs of the analyte may affect the selectivity of fluorescent GSH‐AuNCs and limit their wide applications.…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Trace Metronidazole by Using a Magnetic Molecularly Imprinted Polymer‐based Fluorescent Probe

Tan

Lan

Yin

et al. 2019

Bulletin Korean Chem Soc

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A magnetic molecularly imprinted polymer-based fluorescent (MIP-FL) probe has been synthesized by embedding glutathione-stabilized gold nanoclusters (GSH-AuNCs) and Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) into silica composites. The Fe 3 O 4 NPs/AuNCs@MIPs are characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and UV-Vis absorption spectroscopy. The results show that the spherical Fe 3 O 4 NPs/AuNCs@MIPs contain a core-shell structure with GSH-AuNCs anchored on the surface of silica-protected Fe 3 O 4 NPs. As a fluorescence probe (excitation: 360 nm, emission: 615 nm), Fe 3 O 4 NPs/AuNCs@MIPs can selectively detect metronidazole (MNZ) among other nitroimidazoles (ronidazole, ornidazole, and tinidazole). During the detection, the fluorescence intensity of the MIP-FL probe drops gradually with increasing MNZ concentration. The sensitive linear range of the fluorescence probe is from 0 to 5 μM, and the limit of detection is 4.2 nM. After the recognition sites interact with the template, the occurrence of charge transfer from the GSH-AuNCs to MNZ results in fluorescence quenching. Finally, a real sample test has been performed in spiked milk. Satisfactory recoveries spanning from 96% to 102% indicate that Fe 3 O 4 NPs/AuNCs@MIPs enable highly sensitive detection of MNZ based on fluorescence signal output, while the MIPs also have enrichment potential for the target from complex samples due to the magnetic properties.

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Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation

Cited by 20 publications

References 42 publications

Amphiphilic Au Nanoclusters Modulated by Magnetic Gemini Surfactants as a Cysteine Chemosensor and an MRI Contrast Agent

Amphiphilic Au Nanoclusters Modulated by Magnetic Gemini Surfactants as a Cysteine Chemosensor and an MRI Contrast Agent

Rapid determination of cysteine and chiral discrimination of D-/L-cysteine via the aggregation-induced emission enhancement of gold nanoclusters by Ag+

Selective Detection of Trace Metronidazole by Using a Magnetic Molecularly Imprinted Polymer‐based Fluorescent Probe

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