Rapid determination of cysteine and chiral discrimination of D-/L-cysteine via the aggregation-induced emission enhancement of gold nanoclusters by Ag+

Abstract

Cysteine (Cys) plays vital roles in various physiological and pathological functions.Either deficiency or excess of Cys could lead to severe ailments in human. Identification and determination of Cys are the key issues for the early diagnosis of relevant diseases.This contribution presented a promising potential of fluorescent gold nanoclusters (AuNCs) for Cys determination and D-/L-Cys enantiomer discrimination. Cys determination and discrimination involved three steps. First, as a reducing and capping ligand, glutathione was applied to fabricate weak fluorescent AuNCs. Second, Ag + was introduced to lead the aggregation-induced emission (AIE) to form well-dispersed aggregates. The fluorescence intensity of AuNCs was monitored at excitation/ emission wavelengths of 396/620 nm. Third, Cys was found to quickly bind with Ag + to form a grid network to light up the system via aggregation-induced emission enhancement (AIEE). A novel sensor for sensitive and visually selective detection of Cys was established on the basis of AIEE mechanism. Rapid quantitative determination of Cys was achieved in two minutes via AIEE within the range of 0.5-100 μmol L −1 and a detection limit of 0.365 μmol L −1 . Moreover, due to the specific interactions of D-/L-Cys with mandelic acid and tartaric acid, visual discrimination of D-/L-Cys enantiomers with naked eyes was realized by replacing the organic acid buffer.