Fluorometric determination of agrA gene transcription in methicillin-resistant Staphylococcus aureus with a graphene oxide–based assay using strand-displacement polymerization recycling and hybridization chain reaction

Ning, Yi; Chen, Shanquan; Hu, Jue; Li, Ling; Cheng, Lijuan; Lu, Fangguo

doi:10.1007/s00604-020-04347-y

Cited by 12 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was also successfully applied to detect milk samples with a LOD of 4 × 10 2 CFU/mL [48]. The Lu group proposed two ultrasensitive fluorescent detection strategies for methicillin-resistant S. aureus mRNA based on the synergistic effect of "FAM-SYBR Green I" on the "HCR-GO" platform and further incorporating other cyclic amplification strategies [49,50].…”

Section: Fluorescencementioning

confidence: 99%

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Zhao,

Guo,

et al. 2023

Foods

View full text Add to dashboard Cite

Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy. This has led to the development of more sensitive and accurate methods for detecting pathogenic bacteria. Many signal amplification techniques have been used to improve the sensitivity of foodborne pathogen detection. Among them, hybridization chain reaction (HCR), an isothermal nucleic acid hybridization signal amplification technique, has received increasing attention due to its enzyme-free and isothermal characteristics, and pathogenic bacteria detection methods using HCR for signal amplification have experienced rapid development in the last five years. In this review, we first describe the development of detection technologies for food contaminants represented by pathogens and introduce the fundamental principles, classifications, and characteristics of HCR. Furthermore, we highlight the application of various biosensors based on HCR nucleic acid amplification technology in detecting foodborne pathogens. Lastly, we summarize and offer insights into the prospects of HCR technology and its application in pathogen detection.

show abstract

Section: Fluorescencementioning

confidence: 99%