Fluorometric Determination of Isoniazid and Its Metabolites in Urine by High-Performance Liquid Chromatography Using In-Line Derivatization

Melatonin prevents the free radical and MADD metabolic profiles induced by antituberculosis drugs in an animal model

“…Superoxide is formed during isoniazid oxidation and is thought to be involved in the activation process [9]. Further isoniazid metabolites that have been detected in humans and rats include isonicotinic acid, isonicotinylglycine [10], acetylhydrazine, and diacetylhydrazine [11]. This can also take place via host‐specific enzymes, such as N‐acetyltransferase [12].…”

Spectrophotometric Determination of Isoniazid in Pharmaceutical Sample by Silicomolybdenum Blue

“…1. The molecular the determination of isoniazid, such as titration method [2],HPLC structure of isoniazid method [3,4],electrochemical analysis method [5,6],capillary electrophoretic method [7,8], fluorescence spectrum method [9], etc.In this paper, we developed a novel method for the indirect determination of isoniazid in pharmaceutical sample by silicomolybdenum blue.Under certain reaction conditions,silicomolybdenum yellow could be reduced to silicomolybdenum blue by isoniazid, and the content of isoniazid was determinated indirectly through determinating the absorbance of the silicomolybdenum blue. The maximum absorption wavelength of chromogenic system is 740 nm, good linear relationship is obtained between the absorbance and the concentration of isoniazid in the range of 1.968～27.55 µg/mL,and the equation of the linear regression is A=0.0247+61.824ρ (µg/mL) with a linear correlation coefficient is 0.9995.This proposed method has been successfully applied to determinate of isoniazid in isoniazid tablets, and the results agree well with those obtained by pharmacopoeial method.This proposed method does not need complicated or expensive equipment,and has the advantages of simply,rapidness, convenience, low analytical cost and so on.…”

Quantification of isoniazid and acetylisoniazid in rat plasma and alveolar macrophages by liquid chromatography–tandem mass spectrometry with on-line extraction

“…More advanced HPLC analytical techniques were later developed for qualitative and quantitative analysis of INH and AcINH in biological specimens. These assays used UV [22][23][24][25], fluorimetric [26,27], or electrochemical [28][29][30] methods for detection of INH and its metabolites. Although more specific, the sensitivity of these methods was not very good.…”