Quantification of isoniazid and acetylisoniazid in rat plasma and alveolar macrophages by liquid chromatography–tandem mass spectrometry with on-line extraction

Isoniazid: A Review of Characteristics, Properties and Analytical Methods

“…Ng and colleagues also reported an HPLC-MS/MS method to determination of isoniazid and acetylisoniazid in rat alveolar macrophages. This determination plays an important role Human plasma and urine (Svensson et al, 1985) HPLC-UV Isoniazid, acetylisoniazid, acetylhydrazine, and diacetylhydrazine Isocratic: 10 mM phosphate buffer pH 5.5, methanol, acetonitrile and dichloromethane (73:17: (Walubo et al, 1991) HPLC-UV Human plasma, serum and cerebrospinal fluid (Seifart et al, 1993) HPLC-UV Isoniazid, acetylisoniazid, and hydrazine Gradient: (A) potassium dihydrogen phosphate buffer 50 mM; (B) acetonitrile and isopropanol (4: Tablets and human serum (Khuhawar and Rind, 2002) HPLC-UV Isoniazid, pyrazinamide, and rifampicin Gradient: (A) acetonitrile; (B) 50 mM phosphate buffer pH 3.5 Tablets (Calleri et al, 2002) HPLC-UV (C) 20 mM 1-heptanesulfonic acid sodium pH 2.5 10% A: 8% B: (Chen et al, 2005) HPLC-MS/MS Isoniazid and acetylisoniazid Gradient: (A) methanol; (B) 0.1% formic acid in water 8% A: (Ng et al, 2007) HPLC-MS/MS Isoniazid, ethambutol, pyrazinamide and rifampicin Gradient: (A) methanol and formic acid 0.3%; (B) water and formic acid 0.3% 60% A: Human serum (Song et al, 2007) (Continued on next page) (Hee et al, 2015) because the macrophages are the main immune cell that phagocyte the Mycobacterium tuberculosis (Ng et al, 2007). In another study, an HPLC-MS/MS technique was used for the determination of isoniazid and others antimicrobials in mouse tissues including brain, lung, liver, kidney and small intestine (Fang et al, 2010).…”

Development and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometry method for determination of isoniazid in human plasma

“…Recently, several LC-MS methods have been reported to determine INH in plasma [8-10]. All of these methods used a C 18 column to separate INH.…”

Simultaneous Determination of Isoniazid and Acetylisoniazid in Human Plasma by Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry and Its Application to a Pharmacokinetics Related to N-Acetyltransferase2 Genetic Polymorphism

“…Zhifeng Zhou et al (Zhou et al 2010) reported a simple HPLC procedure (20 min running time) which was not fit for the rapid detection of these analytes. The LC-MS=MS methods for the simultaneous determination of INH and AcINH have been used previously for the determination of the analytes in rat plasma (Ng et al 2007). Considering INH and AcINH are polar compounds, a HILIC hydrophilic interaction liquid chromatography (HILIC) column has been used to chromatographic separation of INH that show more advantages in the resolution and retention of the chromatographic peaks than reversed-phase column (Li et al 2004).…”