Fluorometric determination of the activity of alkaline phosphatase based on the competitive binding of gold nanoparticles and pyrophosphate to CePO4:Tb nanorods

Xu, Ai-Zhen; Zhang, Li; Zeng, Huidan; Liang, Ru‐Ping; Qiu, Jian‐Ding

doi:10.1007/s00604-018-2827-1

Cited by 26 publications

(5 citation statements)

References 43 publications

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“…Due to the fact that the ALP activity can be inhibited by Na 3 VO 4 , PPi cannot be hydrolyzed into PO 4 3– by ALP; the more Na 3 VO 4 that is added, the more ALP activity that is inhibited and the more P 2 O 7 :Ce,Tb nanoparticles that remained, leading to the stronger fluorescence. It can be seen from Figure d that the fluorescence was enhanced gradually with the increasing Na 3 VO 4 concentration, suggesting that the suppression efficiency was promoted by the increased Na 3 VO 4 concentration, and the half-maximal inhibitory concentration (IC 50 ) was calculated to be 65 μM (inset of Figure d), which is comparable with the reported results (Table S1) and demonstrated that the present approach can be used for not only ALP activity detection but also ALP inhibitor screening …”

Section: Resultssupporting

confidence: 86%

Lanthanide Phosphate Nanoparticle-Based One-Step Optical Discrimination of Alkaline Phosphatase Activity

Zeng

Liu

Peng

et al. 2020

ACS Appl. Nano Mater.

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Utilizing the dramatic fluorescence difference between the P 2 O 7 :Ce,Tb and PO 4 :Ce,Tb nanoparticles, we proposed the onestep optical strategy for fast, sensitive identification of alkaline phosphatase (ALP) in the present work. In this strategy, the pyrophosphate (PPi) combined with the rare earth ions Ce 3+ , Tb 3+ for its high affinity to metal ions to generate P 2 O 7 :Ce,Tb nanoparticles, which exhibit the bright characteristic green emission of Tb 3+ , it origins from the efficient energy transfer from Ce 3+ to Tb 3+ , the whole process can almost be finished instantaneously. While in the presence of ALP, the enzyme substrate PPi can be broken down into PO 4 3− , which can also self-assemble with Ce 3+ , Tb 3+ ions to produce PO 4 :Ce,Tb nanoparticles, but with a much weaker green light of Tb 3+ , and the greater ALP concentration, the weaker green light can be obtained. Based on this, we proposed a facile optical platform for ALP sensing, compared with those reported probes, it avoids the burdensome process of nanoparticle synthesis and target measurement. The experiment result shows that the fluorescence intensity of P 2 O 7 :Ce,Tb at 545 nm is linear to the concentration of ALP in a range of 0.25−250 U/L, and the detection limit is calculated to be 0.18 U/L. In addition, the excellent selectivity of the present strategy has been confirmed as well; the ALP activity suppression and the real serum sample test demonstrate its outstanding reliability forcibly.

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Section: Resultssupporting

confidence: 86%

Lanthanide Phosphate Nanoparticle-Based One-Step Optical Discrimination of Alkaline Phosphatase Activity

Zeng

Liu

Peng

et al. 2020

ACS Appl. Nano Mater.

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“…The limit of detection for ALP was estimated to be 0.08 U/L according to 3σ rule. In comparison with the previously reported ALP detection method (Table 1), the proposed method has lower detection limit and is more cost-effective [30][31][32].…”

Section: Determination Of Alp Activity By the Proposed Methodsmentioning

confidence: 80%

Sensitive Fluorescence Assay for the Detection of Alkaline Phosphatase Based on a Cu2+-Thiamine System

Zhao

Liu

2021

Sensors

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The authors describe a novel, facile, and sensitive fluorometric strategy based on a Cu2+-thiamine (Cu2+-TH) system for the detection of alkaline phosphatase (ALP) activity and inhibition. The principle of the method is as follows. Under a basic conditions, TH, which does not exhibit a fluorescence signal, is oxidized into fluorescent thiochrome (TC) by Cu2+. Ascorbic acid 2-phosphate (AAP), which is the enzyme substrate, is hydrolyzed to produce ascorbic acid (AA) by ALP. The newly formed AA then reduces Cu2+ to Cu+, which prevents the oxidation of TH by Cu2+; as a result, the fluorescent signal becomes weaker. On the contrary, in the absence of ALP, AAP cannot reduce Cu2+; additions of Cu2+ and TH result in a dramatic increase of the fluorescent signal. The sensing strategy displays brilliant sensitivity with a detection limit of 0.08 U/L, and the detection is linear in the concentration range of 0.1 to 100 U/L. This approach was successfully applied to ALP activity in human serum samples, indicating that it is reliable and may be applied to the clinical diagnosis of ALP-related diseases.

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“…This approach is extremely sensitive with 0.06 U L −1 limit of detection (LOD) within the linear range from 0.2 to 100 U L −1. 72 …”

Section: Nano Probesmentioning

confidence: 99%

Applications of fluorescent materials in the detection of alkaline phosphatase activity

Guo

Cui

2020

J Biomed Mater Res

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Alkaline phosphatase (ALP) is important in the diagnosis of many diseases. Because ALP is used to detect biomarkers for many diseases, many researchers conduct investigations to develop ALP detection strategies. The use of fluorescent material has attracted attention because of the technique's high sensitivity and the low sample volume required. Herein, we review and discuss the working mechanisms and advantages of four main categories:DNA fluorescent probes, molecular fluorescent probes, chemical coordination-based probes, and nanoparticle probes. Development prospects and trends are also discussed.

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Fluorometric determination of the activity of alkaline phosphatase based on the competitive binding of gold nanoparticles and pyrophosphate to CePO4:Tb nanorods

Cited by 26 publications

References 43 publications

Lanthanide Phosphate Nanoparticle-Based One-Step Optical Discrimination of Alkaline Phosphatase Activity

Lanthanide Phosphate Nanoparticle-Based One-Step Optical Discrimination of Alkaline Phosphatase Activity

Sensitive Fluorescence Assay for the Detection of Alkaline Phosphatase Based on a Cu2+-Thiamine System

Applications of fluorescent materials in the detection of alkaline phosphatase activity

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