Fluorometric determination of the activity of the biomarker terminal deoxynucleotidyl transferase via the enhancement of the fluorescence of silver nanoclusters by in-situ grown DNA tails

Chi, Baozhu; Wang, Chen-Lu; Wang, Zhiqiao; Pi, Ting; Zhong, Xiaoli; Deng, Chao-Qun; Feng, Yuchuan; Li, Zhimei

doi:10.1007/s00604-019-3288-x

Cited by 12 publications

(2 citation statements)

References 23 publications

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“…The same mechanism was used for the detection of terminal deoxynucleotidyl transferase (TdTase), a tissue-specific enzyme involved in the rearrangement of lymphocyte antigen receptor genes on immature lymphocytes and acute lymphoblastic leukaemia cells. 71 Chi et al 72 developed AgNCs templated by a guanine-rich DNA that bind specifically to TdTase, thus being able to quantify the enzymatic activity in a linear range between 1 and 35 mU mL −1 with a LOD of 0.8 mU mL −1 . Another similar approach is to use aptamers as scaffolds for NCs.…”

Section: Fluorescent Nanocluster-based Biomarker Detection Systems: E...mentioning

confidence: 99%

Biomarker sensing platforms based on fluorescent metal nanoclusters

et al. 2021

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Section: Fluorescent Nanocluster-based Biomarker Detection Systems: E...mentioning

confidence: 99%

Biomarker sensing platforms based on fluorescent metal nanoclusters

et al. 2021

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“…In particular, it is known that terminal deoxynucleotidyl transferase (TdT) is able to catalyze the repetitive addition of deoxyribonucleotides to the 3' hydroxyl end (3'-OH) of DNA. [11][12][13][14][15][16] Abnormal expression of TdT in numerous cancer diseases is highly associated with the pathogenesis of these diseases. [17][18][19][20] Therefore, monitoring TdT activity has become increasingly important in clinical diagnosis and treatment.…”

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confidence: 99%

Label-Free Electrochemical Strategy for Probing TdT and Exo I Activity by Silver-Based Square Wave Voltammetry

Zhang

et al. 2019

J. Electrochem. Soc.

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Monitoring DNA-related enzymes activity precisely and reliably is absolutely vital to better grasp their roles in the initiation and progression of cancer, thus further exploring their functions in cancer diagnosis and treat. Here, we establish a label-free electrochemical biosensor for investigating both terminal deoxynucleotidyl transferase (TdT) and exonuclease I (Exo I) activity by silver-based square wave stripping voltammetry (SWV). The strategy is achieved by two attractive points in design: a TdT-derived enzymatic synthesis of randomly arrayed C-rich DNA sequence and an Exo I-guided enzymatic degradation. This C-rich DNA-templated silver nanoclusters (AgNCs) serve as the electrochemical signal probe, and a signal on-off modulated process is displayed for the existence of TdT and Exo I, respectively. On account of AgNCs-based square wave stripping voltammetry, testing TdT has a linear concentration range of 0.001 to 5 U/mL with a detection limit of 0.00044 U/mL (S/N = 3). Additionally, Exo I with a detection limit of 0.027 U/mL (S/N = 3) can be analyzed with a linear range of 0.1 to 500 U/mL. The real application of the proposed method in their inhibitors screening is also discussed well. The biosensor provides a powerful idea for the ultrasensitive bio-analysis and shows broad clinical application prospect.

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