Fluorometric method based on molecular recognition solid-phase extraction for determination of riboflavin in milk and infant formula

Osório, Marcelo V.; Marques, Sara S.; Oliveira, Hugo M.; Barreiros, Luísa; Segundo, Marcela A.

doi:10.1016/j.jfca.2015.10.007

Cited by 19 publications

(6 citation statements)

References 28 publications

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“…The nanoprobe provides a simple approach to creating a ratiometric fluorescent sensor based on the FRET process for the determination of RF in aqueous media. Capillary Electrophoresis/Laser-Induced Fluorescence 0.001-1.33 0.008 [15] HPLC/tandem mass spectrometry 0.011-53.1 0.029 [12] Ag nanoparticles modified with β-CD/Colorimetry 0.16-4.66 0.167 [36] Spectrophotometry/Absorbance at 440 nm 0.797-123 0.79 [11] Graphitic carbon nitride/Fluorimetry (FRET) 0.4-10 0.170 [16] Carbon-dot-codoped N, P/Fluorimetry (FRET) 0.5-50 0.170 [17] g-CNQDs@Zn-MOF/Fluorimetry (FRET) 0-1 0.015 [18] Sulfonated graphene/Fluorimetry (FRET) 0.4-10 1.6 [37] N,S-doped carbon dots/Fluorimetry (FRET) 0.56-7.4 1.9 [19] Graphene-PEG/Fluorescence Quenching 0-6.3 60 [38] Molecularly Imprinted Polymer (PIM)/Fluorimetry Not reported 79.7 [39] Carbon dots (N, COO − )/Fluorimetry (FRET) 0-11 0.025 This work…”

Section: Comparison With Other Methodsmentioning

confidence: 99%

Optimizing the Efficiency of a Cytocompatible Carbon-Dots-Based FRET Platform and Its Application as a Riboflavin Sensor in Beverages

et al. 2021

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In this work, the Förster resonance energy transfer (FRET) between carbon dots (CDs) as energy donors and riboflavin (RF) as an energy acceptor was optimized and the main parameters that characterize the FRET process were determined. The results were successfully applied in the development of an ultrasensitive ratiometric fluorescent sensor for the selective and sensitive determination of RF in different beverages. Water-soluble CDs with a high quantum yield (54%) were synthesized by a facile and direct microwave-assisted technique. The CDs were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, and UV-visible and molecular fluorescence spectroscopy. The study of the FRET process at two donor concentrations showed that the energy transfer efficiency decreases as the donor concentration increases, confirming its dependence on the acceptor:donor ratio in nanoparticle-based systems. The results show the importance of optimizing the FRET process conditions to improve the corresponding output signal. The variation in the ratiometric signal with the concentration of RF showed linearity in a concentration range of 0 to 11 µM with R2 = 0.9973 and a detection limit of 0.025 µM. The developed nanosensor showed good selectivity over other possible types of interference. The sensor was then applied for the determination of RF in beverage samples using the standard addition method with recoveries between 96% and 106%. Preliminary cytocompatibility tests carried out with breast cancer cells (MDA-MB-231) revealed the nanosensor to be cytocompatible in its working concentration regime, even after long incubation times with cells. Altogether, the developed RF determination method was found to be fast, low-cost, highly sensitive, and selective and can be extended to other samples of interest in the biological and food sectors. Moreover, thanks to its long-lasting cytocompatibility, the developed platform can also be envisaged for other applications of biological interest, such as intracellular sensing and staining for live cell microscopy.

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Section: Comparison With Other Methodsmentioning

confidence: 99%

Optimizing the Efficiency of a Cytocompatible Carbon-Dots-Based FRET Platform and Its Application as a Riboflavin Sensor in Beverages

et al. 2021

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“…Electronic spectral studies (in the range 200 to 800 nm) were carried out in SHIMADZU MODEL UV-1800 240V spectrophotometer. BRUKER AVANCE spectrometer operating at 500 MHz was tied up to register 1 H NMR spectra in C2D5OD solvent. Electrochemical analyses were done on CHI electrochemical analyzer 1200B model comprised of Ag/AgCl as reference electrode, glassy carbon as working electrode and platinum as counter electrode.…”

Section: Instrumentsmentioning

confidence: 99%

“…Heavy metal ions and microbe's persistence in the environment is responsible for the deterioration of the quality of ecological system. Separation of metals from multifaceted solutions containing trace amount of metals is achieved by MRT-SPE technique (Molecular recognition technology solid phase extraction) [1]. Essential and non-essential (but harmful) metals may exist in industrial effluent, body fluid, residue of radioactive material and metallurgical extract.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Ferrocene Based Schiff Bases Possessing Different Metal Ion Sensing Aptitude and Partaking Antimicrobial Activity

Dhasarathan

Shunmugaperumal

2022

J. Mex. Chem. Soc.

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Abstract. Schiff bases comprised of highly reactive ferrocene derivatives and normal aromatic moiety have been prepared successfully. Spectral variations noticed in the spectra of newly synthesized receptors for the addition of different metal ions discloses the multi metal ion sensing ability of the prepared sensors. Harmonization of Cu2+ ions with receptor originate as MLCT band in the visible region. Shrewdness made from the data obtained from cyclic voltammetry studies give an idea about the concentration of metal ions needed for effective sensing. In vitro antimicrobial studies and H- bond energy calculation for the interaction between the above sensory materials and proteins of selected microorganisms using molecular docking studies disclosures the antifungal activity of newly prepared materials. Resumen. Bases de Schiff derivadas de grupos ferrocenilos altamente reactivos y grupos aromáticas fueron preparadas exitosamente. La habilidad de los sistemas como sensores para detectar diversos iones metálicos se vió en la variación de las características observadas en sus espectors. La interacción de iones Cu2+ con el receptor produce una banda MLCT en la región visible. Los estudios de voltametría cíclica indican la concentración de los iones metálicos necesaria para una detección eficiente. Estudios antimicrobianos in vitro y cálculos de la energía de puentes de hidrógeno para las interacciones entre los sensores (bases de Schiff) y las proteínas de microorganismos selectos, basados en estudios de acoplamiento molecular, confirman la actividad antifúngica de los nuevos compuestos reportados.

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“…Traditionally, chemical markers are quantified by evaluating their concentrations using conventional analytical methods. For example, the concentrations of AA, Rbf, and HMF are normally quantified by high-performance liquid chromatography (HPLC) methods [ 6 , 7 , 8 , 9 ], and for the quantification of the -SH groups, the Ellman method is usually used [ 10 , 11 ]. These analytical methods do not allow for the continuous and easy monitoring of the process because they are expensive methodologies, require sophisticated equipment and personnel with appropriate professional qualifications, and in most cases require sample extraction or pre-treatment steps, increasing the time needed for analysis.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Portable Fluorescence Spectroscopy System to Monitor Heat Damage in Industrially Processed Milk

Alvarado,

Zamora,

Arango

et al. 2024

Foods

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Heat treatments play a critical role in ensuring the safety and preservation of milk, but it can affect its nutritional and sensory properties. The present paper proposes the use of a portable system based on fluorescence spectroscopy as an alternative method for the quantification of four thermal damage markers at once (hydroxymethylfurfural, sulfhydryl groups, ascorbic acid, and riboflavin). The obtained prediction models using autofluorescent compounds (tryptophan, dityrosine, Maillard compounds, and riboflavin), validated with skimmed milk processed under several industrial conditions, granted the development of a portable and/or online system, allowing for the real-time monitoring of thermal damage and control of the heat treatment process. The results of this study will certainly contribute to the development of new process analytical technologies for the dairy industry, enabling quality control and adjustment of the manufacturing process to ensure safe and high-quality products.

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Fluorometric method based on molecular recognition solid-phase extraction for determination of riboflavin in milk and infant formula

Cited by 19 publications

References 28 publications

Optimizing the Efficiency of a Cytocompatible Carbon-Dots-Based FRET Platform and Its Application as a Riboflavin Sensor in Beverages

Optimizing the Efficiency of a Cytocompatible Carbon-Dots-Based FRET Platform and Its Application as a Riboflavin Sensor in Beverages

Synthesis of Ferrocene Based Schiff Bases Possessing Different Metal Ion Sensing Aptitude and Partaking Antimicrobial Activity

Validation of a Portable Fluorescence Spectroscopy System to Monitor Heat Damage in Industrially Processed Milk

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