Fluorometric study of fluoxetine DNA binding

Kashanian, Soheila; Javanmardi, Sanaz; Chitsazan, Arash; Paknejad, Maliheh; Omidfar, Kobra

doi:10.1016/j.jphotobiol.2012.04.002

Cited by 36 publications

(15 citation statements)

References 46 publications

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“…Intercalative binding mode between small molecules and DNA double strands leads to protection of the entrapped molecules from an ionic quencher, A B thereby decrease in the fluorescent quenching (Kashanian et al, 2012). Accordingly, the magnitude of KSV of the bounded small molecules should be lower than that of the free molecules.…”

Section: Ki Quenching Studiesmentioning

confidence: 99%

Synthesis, spectroscopic studies of novel N-substituted phthalimides and evaluation of their antibacterial, antioxidant, DNA binding and molecular docking studies

Nayab

Pulaganti

Chitta

et al. 2015

Bangladesh J Pharmacol

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A new series of N-substituted phthalimide derivatives were prepared by condensation of appropriate amount of n-amino tetrachlorophthalimide with respective aldehyde in glacial acetic acid. The structural investigation of the synthesized compounds was done by spectroscopic methods (UV-Vis., IR, 1 H and 13 C NMR) and elemental analysis. The antibacterial screening of these compounds was performed against Escherichia coli and Staphylococcus mutans. The synthesized compounds were evaluated for their antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a scavenging agent. The interaction ability of most promising compounds (3a and 3b) with native calf thymus DNA (Ct-DNA) was also studied by means of UV-Vis., circular dichroism (CD), viscosity measurements and thermal studies. The intrinsic binding constants (Kb) of 3a and 3b with Ct-DNA obtained from UV-Vis. absorption studies were 8 × 10 4 and 1 × 10 5 , respectively. Molecular docking of target compounds (3a and 3b) against DNA dodecamer d (CGCGAATTCGCG)2 has been carried out. The test compounds exhibited remarkable antibacterial, antioxidant and DNA binding activities. Article Info

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Section: Ki Quenching Studiesmentioning

confidence: 99%

Synthesis, spectroscopic studies of novel N-substituted phthalimides and evaluation of their antibacterial, antioxidant, DNA binding and molecular docking studies

Nayab

Pulaganti

Chitta

et al. 2015

Bangladesh J Pharmacol

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“…EO showed lower Kb than fluoxetine (FLX), belong to the same antidepressant group (SSRI), which exhibited 6700. According to the report of Kashanian et al [30], the formation of FLX-DNA was not intercalative binding mode. Likely we propose that EO binds to DNA through outside binding such as electrostatic interaction not via intercalation.…”

Section: Discussionmentioning

confidence: 93%

Study of DNA-Binding Activity and Antibacterial Effect of Escitalopram Oxalate, an Extensively Prescribed Antidepressant

2019

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Escitalopram oxalate (EO) is considered as one of the extensively prescribed antidepressant drug in Turkey and some other countries, therefore this research was aimed to study the interaction of the drug with DNA and study of the substance effect on bacterial growth. The absorption value of the drug solution at 238 nm was increased when DNA was added gradually to it and it showed hyperchromism effect. The value obtained for DNA binding constant (Kb) was 0.035 M −1. When we added the CuCl2 2H2O to the mixture, any breakage was not shown in double strand DNA in comparison with control DNA. In addition low concentration of EO couldn’t protect DNA (0.5273 µmole bp) against Hydroxyl free radical (0.12 µmole) although it could protect the DNA when it was at the same or higher concentrations (0.5273, 5.273 and 252.73 µmole) than the DNA concentration. In addition, MIC of the drug for E.coli and Bacillus subtilis was almost 0.185 mM and 0.55 mM respectively. The E.coli strain was killed at concentrations 45, 15, 5 mM while the Bacillus subtilis was stable against all of the concentrations.

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“…The purity of ct-DNA was checked by monitoring the ratio of the absorbance at 260 nm to 280 nm. The solution gave a ratio of A 260 /A 280 = 1.80, which indicates that the DNA was sufficiently free from protein [46,47]. TXNHCH 2 COOH stock solution (3.14x10 -5 mol L -1 ) was prepared by dissolving it in 10mmol L -1 Tris-HCl buffer solution (pH:7.0 containing 100mmol L -1 NaCl).…”

Section: 1chemicals and Reagentsmentioning

confidence: 99%

Studies of the binding mode of TXNHCH 2 COOH with calf thymus DNA by spectroscopic methods

Ataci

Arsu

2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Fluorometric study of fluoxetine DNA binding

Cited by 36 publications

References 46 publications

Synthesis, spectroscopic studies of novel N-substituted phthalimides and evaluation of their antibacterial, antioxidant, DNA binding and molecular docking studies

Synthesis, spectroscopic studies of novel N-substituted phthalimides and evaluation of their antibacterial, antioxidant, DNA binding and molecular docking studies

Study of DNA-Binding Activity and Antibacterial Effect of Escitalopram Oxalate, an Extensively Prescribed Antidepressant

Studies of the binding mode of TXNHCH 2 COOH with calf thymus DNA by spectroscopic methods

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