Focal contacts of normal and RSV-transformed quail cells

Bershadsky, Alexander D.; Tint, I.S.; Neyfakh, Alexander A.; Vasiliev, J. M.

doi:10.1016/0014-4827(85)90467-7

Cited by 86 publications

(20 citation statements)

References 20 publications

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“…The focal contacts (Fig. 3f, open arrow) are recognized as elongated adhesion sites 1-5 µm in length, and point contacts are ϳ10 times smaller (Bershadsky et al, 1985) and less conspicuous (Fig. 3f).…”

Section: Characterization Of ␤1 Integrin and Vinculin Immunoreactivitmentioning

confidence: 98%

“…Focal contacts represent sites of strong anchorage to the extracellular matrix and may reduce the rate of cell migration (Luna and Hitt, 1992). Point contacts are abundant in highly motile cells; after cell transformation with Rous sarcoma virus, the number of focal contacts decreases and numerous point contacts appear over the cell surface, where they become functional sites of anchorage (Bershadsky et al, 1985;Nermut et al, 1991). These structures, alternatively named dot contacts or podosomes, were first assumed to constitute an immature form of focal contact (Burridge et al, 1988).…”

Section: Introductionmentioning

confidence: 98%

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Organization of point contacts in neuronal growth cones

et al. 1999

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Growth cones from rat dorsal root ganglia plated on laminin contain integrin clusters over the entire growth cone surface, and growth cones make transient adhesions at sites called point contacts. We examined, by immunocytochemistry and confocal microscopy, the composition and distribution of point contacts in neuronal growth cones. Vinculin was concentrated in the central domain of growth cones and at the tips of filopodia. Vinculin was specifically associated with integrin clusters at the membrane-substrate interface and thus marked point contacts. The cytoskeletal proteins paxillin and talin colocalized with beta1 integrin in a subpopulation of clusters restricted to the central domain of the growth cone and to the tips of filopodia. The neuron-specific kinase, FAK+ also distributed with the vinculin-positive clusters. The Rho family proteins RhoA, RhoB, and Cdc42 were present in growth cones, and a few Rho clusters were colocalized with vinculin. Examination of proteins resistant to detergent extraction in PC12 cells confirmed the retention of beta1 integrin, paxillin, talin, and vinculin with the cytoskeleton. Moreover, we detected FAK+ and RhoA in the detergent-resistant cytoskeleton, supporting their distribution to point contacts. Our observations indicate that two types of integrin clusters are present in growth cones: those associated with vinculin at the cell substratum interface, and those not associated with vinculin. Point contacts are mature adhesion sites defined by the presence of both beta1 integrin and vinculin, and they are associated with signaling proteins.

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Section: Characterization Of ␤1 Integrin and Vinculin Immunoreactivitmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Organization of point contacts in neuronal growth cones

et al. 1999

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“…There are three main classes of adhesions: (1) focal adhesions, 35 (2) focal complexes, 36 and (3) fibrillar adhesions. All cellular adhesions involve cell surface receptors like integrins, selectins and cadherins, and interactions with the actin cytoskeleton.…”

Section: Cellular Adhesion and Migrationmentioning

confidence: 99%

Mimicking biological phenomena in hydrogel-based biomaterials to promote dynamic cellular responses

Murphy

Lampe

2015

J. Mater. Chem. B

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Hydrogel-based biomaterials, often classified as synthetic or natural, have long been pursued for cell culture, tissue engineering, regenerative medicine, and drug delivery. This classification system is now being blurred as hybrid partially synthetic systems using elements of native and designer molecules gain traction. Partially synthetic polymer gels can offer protection of encapsulated cells or drugs and provide instructional biochemical and/or biophysical cues to cells. To enable cellular interaction however, they must be endowed with bioactive elements. The extracellular matrix (ECM) provides a ''toolbox'' of bioactive moieties that can be incorporated into user-defined synthetic polymer scaffolds to promote cell responses such as migration or cell-derived material responses such as matrix multimerization.Incorporating bioactive elements like cell-adhesive peptides, protease-cleavable sites, and ECM-mimetic mechanical properties such as stiffness and porosity into robust well-characterized hydrogels has been pursued for decades. Through careful selection of linkage chemistries and structured design of material subunits, hydrogels have been created that facilitate a great deal of native cellular functions while retaining the customizable nature of engineered materials. A new thrust has emerged to engineer materials with the innate, dynamic bioresponsive activity of native ECM materials. This review characterizes the cell responsive units of native materials and the literature that has recently incorporated these elements into hydrogel tissue engineering and drug delivery materials to promote cell-controlled dynamic responses, a defining characteristic of native functional tissue.

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“…Time-lapsed studies of the corona distribution presence in response to the substrate may have the potential to be a sensitive marker of early cell/substrate interactions. After 24 h a random distribution of mostly fibrillar like vinculin assemblies were observed on all surfaces, which is characteristic for more mature cell surface contacts [49]. Cell motility was analyzed as velocity in 28 min intervals of single cells to provide a time-lapsed motility overview that is unbiased by change of direction.…”

Section: Initial Cell Interactionsmentioning

confidence: 99%