Folate assay of foods by traditional and tri-enzyme treatments using cryoprotected Lactobacillus casei

Shrestha, Ashok K.; Arcot, Jayashree; Paterson, Janet

doi:10.1016/s0308-8146(00)00210-7

Cited by 60 publications

(57 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This corresponds to the results by Iwatani et al (2003), who found the single enzyme treatment more effective than the trienzyme method in the analyses of spinach and Chinese broccoli. Shrestha et al (2000) also found the single enzyme method more suitable than the trienzyme method. On the other hand, the effectiveness of the trienzyme method was established by several authors, mainly in complex food samples (Tamura et al 1997) and in foods with high contents of protein and starch (De Souza & Eitenmiller 1990;Pfeiffer et al 1997).…”

Section: Methodsmentioning

confidence: 97%

Determination of folates in vegetables and their retention during boiling

Holasová¹,

Fiedlerová²,

Vavreinová³

2008

Czech J. Food Sci.

View full text Add to dashboard Cite

Holasová M., Fiedlerová V., Vavreinová S. (2008): Determination of folates in vegetables and their retention during boiling. Czech J. Food Sci., 26: 31-37.HPLC method for 5-methyltetrahydrofolate (5-MTHF) determination in vegetables was optimised for the folate release from the food matrix. Enzymatic hydrolyses using the subsequent addition of α-amylase, protease, and conjugase from hog kidney, or their combinations, were tested. The highest release values were obtained with the application of enzymes α-amylase and conjugase, amounting to 112.4-127.0% of the values obtained in the processing with sole conjugase. The simultaneous addition of both enzymes and the incubation at pH 4.9 did not suppress the release of folates. Spinach, Chinese cabbage, lettuce, cauliflower, and broccoli contained more than 50 μg of 5-MTHF/100g, whereas less than 25 μg/100 g was found in potatoes, carrot, white cabbage, green and yellow pepper. Individual vegetables differed in the folate retention during their boiling under constant conditions. The highest retention was found in Brussels sprouts, cauliflower, and broccoli. After 8 min boiling more than 75% of the initial amount of 5-MTHF remained in these vegetables. Lower values of 5-MTHF retention, between 37% and 52% of their initial content, were found in spinach, savoy cabbage, and carrot.

show abstract

Section: Methodsmentioning

confidence: 97%

Determination of folates in vegetables and their retention during boiling

Holasová¹,

Fiedlerová²,

Vavreinová³

2008

Czech J. Food Sci.

View full text Add to dashboard Cite

show abstract

“…The protocol used was based on Shrestha, Arcot, and Paterson (2000), Tamura, Mizuno, Johnson, and Jacob (1997) and Rader, Weaver, and Angyal (1998) and used an extraction buffer consisting of 13.61 g of KH 2 PO 4 , 17.42 g of K 2 HPO 4 , 1.0% ascorbic acid and 10 g of L-ascorbic acid in 1000 ml of milliQ water; the pH was adjusted to pH 6.1 using 0.1 M NaOH. All sample extractions were carried out in subdued light and all the glassware was wrapped with aluminium foil to prevent light destruction of folate.…”

Section: Trienzyme Extraction For Total Folate Determinationmentioning

confidence: 99%

Aibika (Abelmoschus manihot L.): Genetic variation, morphology and relationships to micronutrient composition

et al. 2016

Self Cite

View full text Add to dashboard Cite

“…The culture of L. casei ATCC 7469 was obtained from The School of Microbiology and Immunology, UNSW, Sydney, Australia and the preparation of the glycerolcryoprotected culture of L. casei was done according to Wilson and Horne (1982). The use of folic acid (F-7876, Sigma Chemical Co., St. Louis, MO) as a standard, the preparation of standard solutions and sample tubes as well as incubation (37 o C, 16-18 hours), and the absorbance measurement using a spectrophotometer (540 nm) were performed following the procedure introduced by Keagy (1985) and Shrestha et al (2000).…”

Section: Folate Analysismentioning

confidence: 99%

“…This method includes the extraction of folate from the sample matrix, deconjugation of polyglutamates to monoglutamates and detection of the concentration through the turbidimetric bacterial growth of Lactobacillus casei (ATCC 7469). The frequent uses of chicken pancreas and hog kidney as folate conjugase sources have been reported (Gregory 1984;Holt et al 1988;Engelhardt and Gregory 1990;Aiso and Tamura 1998;Shrestha et al 2000), while only few studies have been published regarding the use of human plasma despite its advantages over other conjugase sources. Human plasma is easily available, cheaper, used in small quantity, produces monoglutamates as the end products, and contains low endigenous folate content (Lakshmaiah and Ramasastri 1975a;Goli and Vanderslice 1992), hence it was chosen as a conjugase source in this study.…”

Section: Introductionmentioning

confidence: 99%

Soil Properties of the Eastern Toposequence of Mount Kelimutu, Flores Island, East Nusa Tenggara and Their Potential for Agricultural Use

Hikmatullah¹,

Subagyo²,

Prasetyo³

2016

Indones. J. Agric. Sci.

View full text Add to dashboard Cite

Kelimutu stratovolcano is one of the young volcanoes extensively found in Flores island. Studies on volcanic soil properties and their suitability for agricultural development in the whole island are lacking. The research was conducted to evaluate the relationship of elevation and soil properties, and suitability for agricultural development. Five representative pedons at elevation of 550, 1,000, 1,200, 1,400, and 1,600 m above sea level (asl), respectively, were studied in the field, and 22 soil samples were analyzed in the laboratory. The results indicated that elevation significantly affected soil properties and degree of soil weathering. With decreasing elevation, sand content, amorphous material content, and phosphate retention decreased. Concomitantly, clay content, H2O-pH, exchangeable Ca and Mg, base saturation, and soil-CEC increased. A highly positive correlation was shown between P retention and NaFpH, Al, and (Al+0.5 Fe) contents extracted by acid ammonium oxalate. Soil-CEC also showed significant positive correlation with clay and silt contents, organic carbon, and exchangeable Ca and Mg. Degree of weathering increased with decreasing elevation as reflected by decreasing silt/clay ratio. Soils developed from Entisols (Lithic Udorthents) at 1,600 m asl, to Andisols (Typic Hapludands) at 1,000-1,400 m asl, and Mollisols (Typic Hapludolls) at lower elevation down to 550 m asl. Soils at 1,400-1,600 m asl are unsuitable for agriculture. Soils at 1,000-1,200 m are moderately suitable for food crops, horticulture, and estate crops. Dominant limiting factors are steep slopes and P retention. Soils at 550 m asl are suitable for food and estate crops, and also lowland horticulture.

show abstract

Folate assay of foods by traditional and tri-enzyme treatments using cryoprotected Lactobacillus casei

Cited by 60 publications

References 19 publications

Determination of folates in vegetables and their retention during boiling

Determination of folates in vegetables and their retention during boiling

Aibika (Abelmoschus manihot L.): Genetic variation, morphology and relationships to micronutrient composition

Soil Properties of the Eastern Toposequence of Mount Kelimutu, Flores Island, East Nusa Tenggara and Their Potential for Agricultural Use

Contact Info

Product

Resources

About