Folate (MTHFRC677T andMTRRA66G) gene polymorphisms and risk of prostate cancer: a case-control study with an updated meta-analysis

Abstract

IntroductionMethylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) are the key enzymes of the folate pathway, which involved in the DNA methylation. DNA methylation may affect the stability and integrity of DNA, that supposed to play a pivotal role in carcinogenesis. So, we aimed to investigate the association of MTHFR C677T and MTRR A66G gene polymorphisms with susceptibility to prostate cancer in North Indian population. We also performed meta-analyses of published literatures on these polymorphisms to evaluate their association with prostate cancer.MethodsWe genotyped MTHFR C677T and MTRR A66G gene polymorphisms in 147 prostate cancer cases and 147 healthy controls using PCR-RFLP methods. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated for risk estimation. For meta-analysis different databases were searched and all statistical analysis were performed using Open Meta-Analyst software.ResultsThe present case control study revealed that the T allele (OR= 1.67; 95% CI: 0.99-2.84, p= 0.05), CT genotype (OR= 1.92; 95% CI: 1.06-3.48, p= 0.02), and dominant (TT+CT) model (OR= 1.85; 95% CI: 1.05-3.30, p= 0.03) of MTHFR C677T gene polymorphism and G allele (OR= 1.92; 95% CI: 1.35-2.73, p= 0.0002) of MTRR A66G gene polymorphism were significantly associated with prostate cancer susceptibility. Meta-analyses of MTHFR C677T and MTRR A66G gene polymorphisms showed no significant association between these polymorphisms and prostate cancer risk in overall or in subgroup meta-analysis stratified by ethnicity.ConclusionMTHFR C677T and MTRR A66G gene polymorphisms seem to play a significant role in prostate cancer susceptibility in North Indian population, while results of meta-analysis revealed no association between MTHFR C677T and MTRR A66G gene polymorphisms and prostate cancer susceptibility.

Associations of methylene tetrahydrofolate reductase (MTHFR) polymorphism with hepatocellular carcinoma in Egyptian population

“…Molecular epidemiologic studies have revealed that MTHFR polymorphisms are associated with an increased risk of esophageal cancer, breast cancer [26], gastric cancer [27], hepatocellular carcinoma [28], cervical neoplasia [29], [30], bladder cancer [31], and indolent prostate cancer [32]. Conversely, MTHFR polymorphisms have also been associated with a reduced risk of colon cancer [33], leukemia and lymphoma [34] and highly aggressive prostate cancer [35]. In addition, no association has been found between the C677Tvariant and colon adenoma and lung cancer [36].…”

Associations of Methylenetetrahydrofolate reductase (MTHFR) polymorphism with Hepatocellular carcinoma In Egyptian population.

“…Conversely, MTHFR polymorphisms have also been associated with a reduced risk of colon cancer [47], leukemia and lymphoma[48] and highly aggressive prostate cancer [49]. In addition, no association has been found between the C677Tvariant and colon adenoma and lung cancer [50].…”