Folate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis

Cho, Kyung Chul; Jeong, Ji Hoon; Chung, Hyun Jung; Joe, Cheol O.; Kim, Sung Wan; Park, Tae Gwan

doi:10.1016/j.jconrel.2005.07.015

Cited by 50 publications

(17 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to apply these nanophosphors in bioimaging, amine groups were covalently bonded to PEG by DCC/NHS chemistry. [16] The TEM images shown in Figure 1 indicate the obtained PEGylated upconversion nanophosphors were spherical and monodispersed with mean diameter of 50 +/- 5 nm (the size distribution is shown in Figure S3, supporting information). The AL and PEI coating were shown to be very effective at preventing aggregation.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Brightly PEGylated Luminescent Magnetic Upconversion Nanophosphors for Deep Tissue and Dual MRI Imaging

Chen

Moore

et al. 2013

Small

View full text Add to dashboard Cite

We developed a novel method to fabricate monodispersed biocompatible Yb/Er or Yb/Tm doped β-NaGdF4 upconversion phosphors using polyelectrolytes to prevent irreversible particle aggregation during the conversion of the precursor (Gd2O(CO3)2•H2O:Yb/Er or Yb/Tm) to β-NaGdF4:Yb/Er or Yb/Tm. The polyelectrolyte on the outer surface of nanophosphors also provided an amine tag for PEGylation. This method is also employed to fabricate PEGylated magnetic upconversion phosphors with Fe3O4 as the core and β-NaGdF4 as a shell. These magnetic upconversion nanophosphors have relatively high saturation magnetization (7.0 emu g-1) and magnetic susceptibility (1.7×10-2 emu g-1 Oe-1), providing them with large magnetophoretic mobilities. We have studied their magnetic properties for separation and controlled release in flow, their optical properties for cell labeling, deep tissue imaging, and their T1 and T2-weighted magnetic resonance imaging (MRI) relaxivities. The magnetic upconversion phosphors display both strong magnetophoresis, dual MRI imaging (r1=2.9 mM-1 s-1, r2=204 mM-1 s-1), and bright luminescence under 1 cm chicken breast.

show abstract

Section: Resultsmentioning

confidence: 99%

Synthesis of Brightly PEGylated Luminescent Magnetic Upconversion Nanophosphors for Deep Tissue and Dual MRI Imaging

Chen

Moore

et al. 2013

Small

View full text Add to dashboard Cite

show abstract

“…Since apoptosis is accompanied by the activation of endogenous nucleases, which cleaves chromosomal DNA into oligonucleosomal fragments, apoptotic cells can be recognized by their diminished DNA content within the nuclei (30,31). The extent of apoptosis was determined by appearance of the cells in a sub-G1 phase (DNA content of less than diploid).…”

Section: Resultsmentioning

confidence: 99%

Intracellular Delivery of Heparin Complexed with Chitosan-g-Poly(Ethylene Glycol) for Inducing Apoptosis

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Surface modification of nanosized vectors like nanoparticles is usually used for specific targeting purposes. Various ligands, including antibody,15 folate,16,17 asialoglycoprotein,18 galactose,19,20 mannose,21 epidermal growth factor,22 and transferrin23 have been conjugated with nonviral carriers for cell specificity. It was reported that the asialoglycoprotein receptor (ASGPR) was abundantly expressed in normal hepatocytes and hepatoma cell lines, such as BRL-3A, HepG2, and parental human hepatocellular carcinoma BEL-7402 cells.…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte-targeting gene transfer mediated by galactosylated poly(ethylene glycol)- graft-polyethylenimine derivative

Wang¹,

Su²,

Cai³

et al. 2013

DDDT

View full text Add to dashboard Cite

Biscarbamate cross-linked polyethylenimine derivative (PEI-Et) has been reported as a novel nonviral vector for efficient and safe gene transfer in our previous work. However, it had no cell-specificity. To achieve specific delivery of genes to hepatocytes, galactosylated poly(ethylene glycol)-graft-polyethylenimine derivative (GPE) was prepared through modification of PEI-Et with poly(ethylene glycol) and lactobionic acid, bearing a galactose group as a hepatocyte-targeting moiety. The composition of GPE was characterized by proton nuclear magnetic resonance. The weight-average molecular weight of GPE measured with a gel permeation chromatography instrument was 9489 Da, with a polydispersity of 1.44. GPE could effectively condense plasmid DNA (pDNA) into nanoparticles. Gel retardation assay showed that GPE/pDNA complexes were completely formed at weigh ratios (w/w) over 3. The particle size of GPE/pDNA complexes was 79–100 nm and zeta potential was 6–15 mV, values which were appropriate for cellular uptake. The morphology of GPE/pDNA complexes under atomic force microscopy appeared spherical and uniform in size, with diameters of 53–65 nm. GPE displayed much higher transfection efficiency than commercially available PEI 25 kDa in BRL-3A cell lines. Importantly, GPE showed good hepatocyte specificity. Also, the polymer exhibited significantly lower cytotoxicity compared to PEI 25 kDa at the same concentration or weight ratio in BRL-3A cell lines. To sum up, our results indicated that GPE might carry great potential in safe and efficient hepatocyte-targeting gene delivery.

show abstract

Folate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis

Cited by 50 publications

References 32 publications

Synthesis of Brightly PEGylated Luminescent Magnetic Upconversion Nanophosphors for Deep Tissue and Dual MRI Imaging

Synthesis of Brightly PEGylated Luminescent Magnetic Upconversion Nanophosphors for Deep Tissue and Dual MRI Imaging

Intracellular Delivery of Heparin Complexed with Chitosan-g-Poly(Ethylene Glycol) for Inducing Apoptosis

Hepatocyte-targeting gene transfer mediated by galactosylated poly(ethylene glycol)- graft-polyethylenimine derivative

Contact Info

Product

Resources

About