Foliar-applied potassium improved mycorrhizal Goji (Lycium barbarum L.) growth of the potassium free - compartment in a compartmented culture system

Han, Xia; Du, Xiongming; Wu, Yunxuan; Wei, Man; Yuan, Gu; Aba, Xiaoluo; Tang, Ming; Zhang, Haoqiang

doi:10.1016/j.scienta.2021.110681

Cited by 11 publications

(17 citation statements)

References 38 publications

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“…However, the influence of the AM symbiosis on plant potassium accumulation has received much less attention than that on phosphorus and nitrogen accumulation, and might be due to the strong regulation of the potassium concentration by the plant ( Wang and Wu, 2013 ). Inoculation of R. irregularis and application of potassium increased L. barbarum growth ( Table 1 ) and potassium contents ( Table 2 ), which was consistent with previous studies ( Liu et al., 2019 ; Han et al., 2022a ; Han et al., 2022b ). These results highlight the positive roles of AM fungi and extra potassium in plant growth and potassium accumulation ( Garcia et al., 2017 ; Liu et al., 2019 ; Han et al., 2022b ).…”

Section: Discussionsupporting

confidence: 91%

“…Highly accumulated potassium in AM fungal structures (spores, hyphae, and vesicles) and in some plant tissues implies that AM fungi facilitate potassium uptake and transport to the host plant ( Garcia and Zimmermann, 2014 ). With consideration of the important role of phosphate transport in maintenance of the AM symbiosis ( Smith et al., 2003 ; Javot et al., 2007 ), extra potassium is documented to increase phosphorus transport via the mycorrhizal pathway ( Han et al., 2022b ).…”

Section: Introductionmentioning

confidence: 99%

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LbKAT3 may assist in mycorrhizal potassium uptake, and overexpression of LbKAT3 may promote potassium, phosphorus, and water transport from arbuscular mycorrhizal fungi to the host plant

Han

Zhou

et al. 2023

Front. Plant Sci.

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Potassium plays important roles in most plant physiological processes. Arbuscular mycorrhizal (AM) fungi promote plant water and mineral nutrient acquisition to promote plant growth. However, few studies have focused on the effect of AM colonization on potassium uptake by the host plant. In this study, the effects of an AM fungus (Rhizophagus irregularis) and potassium concentration (0, 3, or 10 mM K+) on Lycium barbarum were evaluated. A split-root test with L. barbarum seedlings was conducted, and the potassium uptake capacity of LbKAT3 was verified in yeast. A tobacco line overexpressing LbKAT3 was generated and mycorrhizal functions under two potassium concentrations (0.2 and 2 mM K+) were studied. Inoculation of R. irregularis and application of potassium increased the dry weight, and potassium and phosphorus contents of L. barbarum, and increased the colonization rate and arbuscule abundance of R. irregularis. In addition, the expression of LbKAT3 and AQP genes in L. barbarum was upregulated. Inoculation of R. irregularis induced LbPT4, Rir-AQP1, and Rir-AQP2 expression, and application of potassium upregulated the expression of these genes. Inoculation with the AM fungus locally regulated the expression of LbKAT3. Inoculation of R. irregularis improved the growth, and potassium and phosphorus contents, and induced NtPT4, Rir-AQP1, and Rir-AQP2 expression in tobacco overexpressing LbKAT3 under both potassium concentrations. Overexpression of LbKAT3 in tobacco improved the growth, potassium accumulation, and AM colonization, and upregulated the expression of NtPT4 and Rir-AQP1 in mycorrhizal tobacco. The results suggest that LbKAT3 may assist in mycorrhizal potassium uptake, and overexpression of LbKAT3 may promote potassium, phosphorus, and water transport from the AM fungus to tobacco.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

LbKAT3 may assist in mycorrhizal potassium uptake, and overexpression of LbKAT3 may promote potassium, phosphorus, and water transport from arbuscular mycorrhizal fungi to the host plant

Han

Zhou

et al. 2023

Front. Plant Sci.

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“…The net photosynthesis rate (Pn) was measured one day before harvest with a Li−6400 potable open flow gas-exchange system (Li-Cor Inc. Lincoln, NE, United States). The measurement conditions were as follows: photosynthetically active irradiation, 500 mmol/m 2 s; temperature, 25 • C; and CO 2 concentration, 400 mmol/mol [27].…”

Section: Net Photosynthesis Rate and Root Viability Measurementsmentioning

confidence: 99%

“…The frozen roots that were stored at −80 • C were homogenized and ground into powder with a mortar and pestle and liquid nitrogen and used for total RNA extraction, which was carried out using an E.Z.N.ATM Plant RNA kit (Omega Bio-Tek, Norcross, GA, USA). First-strand cDNA synthesis was obtained from 1 µg of total RNA using HiScript ® II Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, Nanjing, China) following the supplier's instructions [27].…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

Symbiosis of Arbuscular Mycorrhizal Fungi and Lycium barbarum L. Prefers NO3− over NH4+

et al. 2023

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Nitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested that AM fungi mediate N uptake in plants. However, there are fewer studies on the influence of AM fungi on the response of Lycium barbarum, a medicinal plant in northwest China, under different N-addition conditions. In this study, the effect of Rhizophagus irregularis, N forms (NO3− and NH4+), and N levels (1.5, 7.5, 15, 30 mM) on the performance of L. barbarum was evaluated through a pot experiment. The application of R. irregularis significantly improved L. barbarum biomass, net photosynthetic rate, and root tissue viability under adequate NO3− and NH4+ supplies, and mycorrhizal plants showed better performance under NO3− supply. AM colonization enhanced N acquisition under adequate NO3− supply and strongly induced the expression of LbAMT3-1 in L. barbarum roots. Based on these results, we propose that NO3−-dominated N supply favors mycorrhizal symbiosis to a greater extent than NH4+; this study provides a basis for maintaining beneficial AM symbiosis during nitrogen fertilizer use in arable land.

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“…Several studies have demonstrated that the transpiration of host plants is the driving force for the transport of nutrients (e.g., P, K, and N) from AM fungi to host plant roots [39][40][41] and the suppression of host plant transpiration can decelerate nutrient translocation in proportion to the level of suppression [42]. A previous study has proposed that AM fungi are capable of transferring Pb to the host plant by stimulating transpiration flow [27].…”

Section: Introductionmentioning

confidence: 99%

Pb Transfer Preference of Arbuscular Mycorrhizal Fungus Rhizophagus irregularis in Morus alba under Different Light Intensities

Ren

Zhang

Jin

et al. 2022

JoF

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Arbuscular mycorrhizal (AM) fungi can improve the lead (Pb) tolerance of host plants and accumulate intensive Pb in mycorrhizal roots. However, the detailed contribution of AM fungal extraradical hyphae to the plants’ Pb uptake remains unknown. In this study, mulberry (Morus alba) colonized by the AM fungus (Rhizophagus irregularis) with light treatments were linked by fungal extraradical hyphae using a three-compartment system (pot test), and their differences in responding to Pb application were compared. Shading inhibited mulberry photosynthesis and the growth of mulberry. In this study, Pb application did not affect the colonization of R. irregularis when symbiosis had already formed as the root was not exposed to Pb during the colonization and formation of the AM fungal hyphae network. The R. irregularis preferred to transfer more Pb to the unshaded mulberry than to the shaded mulberry, a condition capable of providing more C supply for fungal survival than to low-light mulberry. The Pb transferred through the mycorrhizal pathway to mulberry had low mobility and might be compartmented in the root by R. irregularis until exceeding a threshold. The relatively high expressions of MaABCG16 with high Pb concentrations in plants suggest that MaABCG16 might play an important role in Pb translocation.

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Foliar-applied potassium improved mycorrhizal Goji (Lycium barbarum L.) growth of the potassium free - compartment in a compartmented culture system

Cited by 11 publications

References 38 publications

LbKAT3 may assist in mycorrhizal potassium uptake, and overexpression of LbKAT3 may promote potassium, phosphorus, and water transport from arbuscular mycorrhizal fungi to the host plant

LbKAT3 may assist in mycorrhizal potassium uptake, and overexpression of LbKAT3 may promote potassium, phosphorus, and water transport from arbuscular mycorrhizal fungi to the host plant

Symbiosis of Arbuscular Mycorrhizal Fungi and Lycium barbarum L. Prefers NO3− over NH4+

Pb Transfer Preference of Arbuscular Mycorrhizal Fungus Rhizophagus irregularis in Morus alba under Different Light Intensities

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