Folic Acid Remodels Chromatin on Hes1 and Neurog2 Promoters during Caudal Neural Tube Development

Ichi, Shunsuke; Costa, Fabrício F.; Bischof, Jared M.; Nakazaki, Hiromichi; Shen, Yueh Wei; Boshnjaku, Vanda; Sharma, Saurabh; Mania-Farnell, Barbara; McLone, David G.; Tomita, Tadanori; Soares, Marcelo B.; Mayanil, C. Shekhar

doi:10.1074/jbc.m110.126714

Cited by 84 publications

(80 citation statements)

References 32 publications

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“…[26][27][28][29] FA has been shown to rescue neural tube defects in Sp -/-embryos. 19 The question addressed in this work is whether FA remodels chromatin on key stem cell maintenance and differentiation genes in DRG cells. Ichi and colleagues 13 found that FA acid remodels chromatin on Hes1 and Neurog2 promoters during caudal neural tube development.…”

Section: Resultsmentioning

confidence: 99%

“…ChIP assays on undifferentiated or differentiated ND7 cells in the absence or presence of FA (200 μg/ml) were performed as described in Ichi et al 19 PCR was performed with primers for rat Hes1 and Ngn2 promoter regions. All ChIP samples were tested for false-positive PCR amplification by sequencing the 200 bp amplified product to ascertain specificity of Pax3 binding to cis-regulatory elements.…”

Section: Antibodiesmentioning

confidence: 99%

“…15,16 The biological rational for using the ND7 cell line in this study was that these hybrid cells act like DRG sensory neurons, upregulating the POU transcription factor Brn3a upon differentiation into neurons 17 and exhibiting decreased levels of Pax3. 14,18,19 Brn3a is required for correct axon growth, target innervation and survival of DRG neurons, 20 whereas Pax3 is an essential paired-homeodomaincontaining transcription factor necessary for neural tube closure and morphogenesis of migratory neural crest and myoblast lineages. Pax3 regulates Hes1 and Neurog2 expression in caudal neural tube development.…”

Section: Introductionmentioning

confidence: 99%

“…To validate that ND7 cells are an appropriate model system to study chromatin remodeling during proliferation and differentiation, we performed ChIP assays on wild-type (WT) mouse E10.5 caudal neural tube derived neurospheres, an established model system. 19 H3K9Ac and H3K27me2 antibodies and Hes1 primers were used to amplify immunoprecipitated DNA from E10.5 neurospheres. FA treatment of neurospheres significantly increased H3K9Ac association at the Hes1 promoter in differentiated (p < 0.001) neurospheres.…”

mentioning

confidence: 99%

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Epigenetic regulation of sensory neurogenesis in the dorsal root ganglion cell line ND7 by folic acid

Boshnjaku

Ichi²,

Shen³

et al. 2011

Epigenetics

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Section: Resultsmentioning

confidence: 99%

Section: Antibodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Epigenetic regulation of sensory neurogenesis in the dorsal root ganglion cell line ND7 by folic acid

Boshnjaku

Ichi²,

Shen³

et al. 2011

Epigenetics

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“…Кроме того, несколько эпидемиологических и молекулярных исследо-ваний также связывают употребление фолата с эпигене-тическим изменением за счет метилирования ДНК с раз-витием нервной трубки, включая активацию рецепторов фолата (Folr1) и регенерацию спинного мозга [50,51]. С целью понимания ФК-индуцированного эпигенетиче-ского механизма регуляции закрытия нервной трубки бы-ло проведено исследование на эмбрионах Splotch (Sp-/-), показавшее, что употребление матерью фолата в перикон-цепционный период снижает количество маркеров мети-лирования H3K27 и ремоделирует хроматин в промоторах Hes1 и Neurog2 -генах, необходимых для развития нерв-ной трубки [52].…”

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Role of folic acid in the prevention of fetal nervous system diseases

Шеманаева¹,

Voevodin²

2015

Ross. vestn. akush.-ginekol.

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Обзор литературы последних лет касается чрезвычайно важной медицинской, социальной и экономической проблемы -профилактики дефектов нервной трубки плода -открытых и закрытых форм spina bifida, краниальных менингоцеле энцефалоцеле. Особое внимание уделяется рассмотрению роли фолатов в профилактике развития дефектов нервной трубки плода. Описан метаболизм фолиевой кислоты, который модулируется несколькими фолатными коэнзимами. Подчеркнуто, что применение фолиевой кислоты может привести к изменениям в метилировании ДНК и модификации гистонов, т.е. фолиевая кислота является нутритивным эпигенетическим фактором. Периконцепционное употребление фолиевой кислоты позволяет снизить частоту дефектов нервной трубки плода. Назначение поливитаминного препарата Элевит Пронаталь с целью профилактики дефицита фолиевой кислоты женщинам в период планирования и вынашивания беременности эффективно для благоприятного течения беременности и нормального развития плода.Авторы информируют об отсутствии конфликта интересов. Ключевые слова: фолиевая кислота, метилирование ДНК, беременность, дефекты нервной трубки, пороки развития плода, профилактика дефектов нервной трубки.The review of the recent literature concerns the supremely important medical, social, and economic problem to prevention of fetal neural tube defects, such as open and closed spina bifida, cranial meningocele, or encephalocele. Particular emphasis is placed on the consideration of the role of folates in the prevention of fetal neural tube defects. The folic acid metabolism modulated by several folate coenzymes is described. It is emphasized that folic acid intake may lead to changes in DNA methylation and histone modification, i.e. folic acid is a nutritional epigenetic factor. The periconceptional use of folic acid allows the incidence of fetal neural tube defects to be reduced. The multivitamin preparation Elevit Pronatal given to prevent folic acid deficiency in women during pregnancy planning and pregnancy is effective for its favorable course and normal fetal development. The authors declare no conflicts of interest.

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Nucleic acid methylation and orofacial morphogenesis

Seelan

Pisano

Greene

2019

Birth Defects Research

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In this review, we highlight the current state of knowledge of the diverse roles nucleic acid methylation plays in the embryonic development of the orofacial region and how aberrant methylation may contribute to orofacial clefts. We also consider the role of methylation in the regulation of neural crest cell function as it pertains to orofacial ontogeny. Changes in DNA methylation, as a consequence of environmental effects, have been observed in the regulatory regions of several genes, potentially identifying new candidate genes for orofacial clefting and opening promising new avenues for further research. While the focus of this review is primarily on the nonsyndromic forms of orofacial clefting, syndromic forms are briefly discussed in the context of aberrant nucleic acid methylation. K E Y W O R D Sbirth defects, epigenetics, neural crest cells, nucleic acid methylation, orofacial clefts

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Folic Acid Remodels Chromatin on Hes1 and Neurog2 Promoters during Caudal Neural Tube Development

Cited by 84 publications

References 32 publications

Epigenetic regulation of sensory neurogenesis in the dorsal root ganglion cell line ND7 by folic acid

Epigenetic regulation of sensory neurogenesis in the dorsal root ganglion cell line ND7 by folic acid

Role of folic acid in the prevention of fetal nervous system diseases

Nucleic acid methylation and orofacial morphogenesis

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